Quorum-quenching potential of recombinant PvdQ-engineered bacteria for biofilm formation

International Microbiology - Tập 26 - Trang 639-650 - 2023
Junlin Li1,2,3, Zhifei Li2,3, Jun Xie2,3, Yun Xia2,3, Wangbao Gong2,3, Jingjing Tian2,3, Kai Zhang2,3, Ermeng Yu2,3, Guangjun Wang2,3
1College of Fisheries and Life Science, Shanghai Ocean University, Nanhui New City, Shanghai, People’s Republic of China
2Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Guangzhou, People’s Republic of China
3Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, China

Tóm tắt

Quorum sensing (QS) is a core mechanism for bacteria to regulate biofilm formation, and therefore, QS inhibition or quorum quenching (QQ) is used as an effective and economically feasible strategy against biofilms. In this study, the PvdQ gene encoding AHL acylase was introduced into Escherichia coli (DE3), and a PvdQ-engineered bacterium with highly efficient QQ activity was obtained and used to inhibit biofilm formation. Gene sequencing and western blot analysis showed that the recombinant pET-PvdQ strain was successfully constructed. The color reaction of Agrobacterium tumefaciens A136 indicated that PvdQ engineering bacteria had shown strong AHL signal molecule quenching activity and significantly inhibited the adhesion (motility) of Pseudomonas aeruginosa and biofilm formation of activated sludge bacteria in Membrane Bio-Reactor (MBR; inhibition rate 51–85%, p < 0.05). In addition, qRT-PCR testing revealed that recombinant PvdQ acylase significantly reduced the transcription level of QS biofilm formation-related genes (cdrA, pqsA, and lasR; p < 0.05). In this study, QQ genetically engineered bacteria enhanced by genetic engineering could effectively inhibit the QS signal transduction mechanism and have the potential to control biofilm formation of pathogenic bacteria in the aquaculture environment, providing an environmentally friendly and alternative antibiotic strategy to suppress biofilm contamination.

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International Microbiology

Tập 26

639-650

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