Microbial production of sabinene—a new terpene-based precursor of advanced biofuel

Microbial Cell Factories - Tập 13 - Trang 1-10 - 2014
Haibo Zhang1, Qiang Liu2, Yujin Cao1, Xinjun Feng1, Yanning Zheng1, Huibin Zou1, Hui Liu1, Jianming Yang1, Mo Xian1
1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Laoshan District, China
2College of Food Science, Sichuan Agricultural University, Yaan, China

Tóm tắt

Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. In this study, sabinene was significantly produced by assembling a biosynthetic pathway using the methylerythritol 4-phosphate (MEP) or heterologous mevalonate (MVA) pathway combining the GPP and sabinene synthase genes in an engineered Escherichia coli strain. Subsequently, the culture medium and process conditions were optimized to enhance sabinene production with a maximum titer of 82.18 mg/L. Finally, the fed-batch fermentation of sabinene was evaluated using the optimized culture medium and process conditions, which reached a maximum concentration of 2.65 g/L with an average productivity of 0.018 g h-1 g-1 dry cells, and the conversion efficiency of glycerol to sabinene (gram to gram) reached 3.49%. This is the first report of microbial synthesis of sabinene using an engineered E. coli strain with the renewable carbon source as feedstock. Therefore, a green and sustainable production strategy has been established for sabinene.

Tài liệu tham khảo

Peralta-Yahya PP, Keasling JD: Advanced biofuel production in microbes. Biotech J. 2010, 5: 147-162. 10.1002/biot.200900220. 10.1002/biot.200900220.

Piper PW: The heat shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap. FEMS Microbiol Lett. 1995, 134: 121-127. 10.1111/j.1574-6968.1995.tb07925.x.

Jiang X, Zhang H, Yang J, Liu M, Feng H, Liu X, Cao Y, Feng D, Xian M: Induction of gene expression in bacteria at optimal growth temperatures. Appl Microbiol Biotechnol. 2013, 97 (12): 5423-5431. 10.1007/s00253-012-4633-8.

Behr A, Johnen L: Myrcene as a natural base chemical in sustainable chemistry: a critical review. ChemSusChem. 2009, 2: 1072-1095. 10.1002/cssc.200900186.

Brown HC, Ramachandran PV: Asymmetric reduction with chiral organoboranes based on. alpha.-pinene. Accounts Chem Res. 1992, 25: 16-24. 10.1021/ar00013a003. 10.1021/ar00013a003.

Kirby J, Keasling JD: Biosynthesis of plant isoprenoids: perspectives for microbial engineering. Annu Rev Plant Biol. 2009, 60: 335-355. 10.1146/annurev.arplant.043008.091955.

Ryder JA: Patent US 7, 935, 156 B2 - Jet fuel compositions and methods of making and using same. 2011

Rude MA, Schirmer A: New microbial fuels: a biotech perspective. Curr Opin Microbiol. 2009, 12: 274-281. 10.1016/j.mib.2009.04.004.

Chen H-T, Lin M-S, Hou S-Y: Multiple-copy-gene integration on chromosome of Escherichia coli for beta-galactosidase production. Korean J Chem Eng. 2008, 25: 1082-1087. 10.1007/s11814-008-0177-0.

Steinbüchel A: Production of rubber-like polymers by microorganisms. Curr Opini Microbiol. 2003, 6: 261-270. 10.1016/S1369-5274(03)00061-4.

Reiling KK, Yoshikuni Y, Martin VJJ, Newman J, Bohlmann J, Keasling JD: Mono and diterpene production in Escherichia coli. Biotechnol Bioeng. 2004, 87: 200-212. 10.1002/bit.20128.

Carter OA, Peters RJ, Croteau R: Monoterpene biosynthesis pathway construction in Escherichia coli. Phytochemistry. 2003, 64: 425-433. 10.1016/S0031-9422(03)00204-8.

Peralta-Yahy PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS: Identification and microbial production of a terpene-based advanced biofuel. Nat commun. 2011, 2: 483-

Bokinsky G, Peralta-Yahya PP, George A, Holmes BM, Steen EJ, Dietrich J, Lee TS, Tullman-Ercek D, Voigt CA, Simmons BA, Keasling JD: Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011, 108: 19949-19954. 10.1073/pnas.1106958108.

Chang MCY, Keasling JD: Production of isoprenoid pharmaceuticals by engineered microbes. Nat Chem Biol. 2006, 2: 674-681. 10.1038/nchembio836.

Singh SK, Strobel GA, Knighton B, Geary B, Sears J, Ezra D: An endophytic Phomopsis sp. possessing bioactivity and fuel potential with its volatile organic compounds. Microb Ecol. 2011, 61: 729-739. 10.1007/s00248-011-9818-7.

Schmidt-Dannert C, Umeno D, Arnold FH: Molecular breeding of carotenoid biosynthetic pathways. Nat Biotechnol. 2000, 18: 750-753. 10.1038/77319.

Clomburg JM, Gonzalez R: Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology. Appl Microbiol Biotechnol. 2010, 86: 419-434. 10.1007/s00253-010-2446-1.

Leonard E, Lim K-H, Saw P-N, Koffas MAG: Engineering central metabolic pathways for high-level flavonoid production in Escherichia coli. Appl Environ Microbiol. 2007, 73: 3877-3886. 10.1128/AEM.00200-07.

Burke C, Croteau R: Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization. Arch Biochem Biophys. 2002, 405: 130-136. 10.1016/S0003-9861(02)00335-1.

Yan YC, Liao J: Engineering metabolic systems for production of advanced fuels. J Ind Microbiol Biotechnol. 2009, 36: 471-479. 10.1007/s10295-009-0532-0.

Fujisaki S, Nishino T, Katsuki H: Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis. J Biochem. 1986, 99: 1327-1337.

Yang J, Xian M, Su S, Zhao G, Nie Q, Jiang X, Yanning Z, Liu W: Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli. PloS One. 2012, 7: e33509- 10.1371/journal.pone.0033509.

Martin VJJ, Pitera DJ, Withers ST, Newman JD, Keasling JD: Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003, 21: 796-802. 10.1038/nbt833.

Zhao Y, Yang J, Qin B, Li Y, Sun Y, Su S, Xian M: Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol. 2011, 90: 1915-19922. 10.1007/s00253-011-3199-1.

Schmidt FR: Optimization and scale up of industrial fermentation processes. Appl Microbiol Biotechnol. 2005, 68: 425-435. 10.1007/s00253-005-0003-0.

Ahamad M, Panda B, Javed S, Ali M: Production of mevastatin by solid-state fermentation using wheat bran as substrate. Res J Microbiol. 2006, 1: 443-447.

Alexeeva YV, Ivanova EP, Bakunina IY, Zvaygintseva TN, Mikhailov VV: Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549T. Lett Appl Microbiol. 2002, 35: 343-346. 10.1046/j.1472-765X.2002.01189.x.

Torija MJ, Beltran G, Novo M, Poblet M, Rozès N, Guillamón JM, Mas A: Effect of the nitrogen source on the fatty acid composition of Saccharomyces cerevisiae. Food Microbiol. 2003, 20: 255-258. 10.1016/S0740-0020(02)00091-6.

Hunke S, Betton J-M: Temperature effect on inclusion body formation and stress response in the periplasm of Escherichia coli. Mol Microbiol. 2003, 50: 1579-1589. 10.1046/j.1365-2958.2003.03785.x.

Groota NS, Ventura S: Effect of temperature on protein quality in bacterial inclusion bodies. FEBS Lett. 2006, 580: 6471-6476. 10.1016/j.febslet.2006.10.071.

Donovan R, Robinson C, Glick B: Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter. J Ind Microbiol Biotechnol. 1996, 16: 145-154.

Bentley WE, Mirjalili N, Andersen DC, Davis RH, Kompala DS: Plasmid‒encoded protein: the principal factor in the “metabolic burden” associated with recombinant bacteria. Biotechnol Bioeng. 1990, 35: 668-681. 10.1002/bit.260350704.

Glick BR: Metabolic load and heterologous gene expression. Biotechnol Adv. 1995, 13: 247-261. 10.1016/0734-9750(95)00004-A.

Dunlop MJ: Engineering microbes for tolerance to next-generation biofuels. Biotechnol Biofuels. 2011, 4: 32- 10.1186/1754-6834-4-32.

Holtwick R, Meinhardt F, Keweloh H: cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8. Appl Environ Microbiol. 1997, 63: 4292-4297.

Ataei SA, Vasheghani-Farahani E: In situ separation of lactic acid from fermentation broth using ion exchange resins. J Ind Microbiol Biotechnol. 2008, 35: 1229-1233. 10.1007/s10295-008-0418-6.

Wang D, Li Q, Song Z, Zhou W, Su Z, Xing J: High cell density fermentation via a metabolically engineered Escherichia coli for the enhanced production of succinic acid. J Chem Technol Biotechnol. 2011, 86: 512-518. 10.1002/jctb.2543. 10.1002/jctb.2543.

Chen N, Huang J, Feng Z, Yu L, Xu Q, Wen T: Optimization of fermentation conditions for the biosynthesis of L-Threonine by Escherichia coli. Appl Biochem Biotechnol. 2009, 158: 595-604. 10.1007/s12010-008-8385-y.

Yang J, Zhao G, Sun Y, Zheng Y, Jiang X, Liu W, Xian M: Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli. Bioresour Technol. 2012, 104: 642-647.

Sambrook J, Russell DW: Molecular cloning: a laboratory manual. 2001, Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 3

Jiang X, Yang J, Zhang H, Zou H, Wang C, Xian M: In vtro assembly of multiple DNA fragments using successive hybridization. PLoS ONE. 2012, 7: e30267- 10.1371/journal.pone.0030267.

Kolb B: Headspace sampling with capillary columns. J Chromatogr A. 1999, 842: 163-205. 10.1016/S0021-9673(99)00073-4.

Tashiro Y, Takeda K, Kobayashi G, Sonomoto K, Ishizaki A, Yoshino S: High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-stat continuous butyric acid and glucose feeding method. J Biosci Bioeng. 2004, 98: 263-268.

Thông tin
Thông tin xuất bản

Microbial Cell Factories

Tập 13

1-10

Thông tin tác giả