Effect of temperature on protein quality in bacterial inclusion bodies

FEBS Letters - Tập 580 - Trang 6471-6476 - 2006
Natalia Sánchez de Groot1, Salvador Ventura1,2
1Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain
2Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bioquimica, E-08193 Bellaterra, Spain

Tóm tắt

Increasing evidence indicates that protein aggregation in bacteria does not necessarily imply loss of biological activity. Here, we have investigated the effect of growth‐temperature on both the activity and stability of the inclusion bodies formed by a point‐mutant of Aβ42 Alzheimer peptide, using green fluorescent protein as a reporter. The activity in the aggregates inversely correlates with the temperature. In contrast, inclusion bodies become more stable in front of chemical denaturation and proteolysis when temperature increases. Overall, the data herein open new perspectives in protein production, while suggesting a kinetic competition between protein folding and aggregation during recombinant protein expression.

Tài liệu tham khảo

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Thông tin xuất bản

FEBS Letters

Tập 580

6471-6476

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