Effects of Dietary Supplementation with κ-Selenocarrageenan on the Selenium Accumulation and Intestinal Microbiota of the Sea Cucumbers Apostichopus japonicus

Biological Trace Element Research - Tập 199 - Trang 2753-2763 - 2020
Kai Wang1, Lina Liu2, Yingying He1, Changfeng Qu1,3, Jinlai Miao1,2,3
1Key Laboratory of Marine Eco-Environmental Science and Technology, The First Institute of Oceanography, Ministry of Natural Resources, Qingdao, China
2Department of Specialty Medicine, School of Basic Medicine, Qingdao University, Qingdao, China
3Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China

Tóm tắt

A 30-day feeding trial was conducted to investigate the effect of κ-selenocarrageenan on the growth performance, selenium accumulation, antioxidant capacity, and intestinal microbiota of sea cucumbers Apostichopus japonicus, with different sizes (70 g ± 10 g and 100 g ± 10 g). Sea cucumbers of each size were randomly assigned into two groups; a diet without supplemented κ-selenocarrageenan was referred to as a control diet, or supplemented with κ-selenocarrageenan at selenium (Se) levels of 2.0 μg/g. Selenium accumulation in the body wall and intestine was determined on days 0, 10, 20, and 30. The survival rate (SR) was significantly higher in the κ-selenocarrageenan-treated group (Se group) than in the control group. After 30 days of feeding, κ-selenocarrageenan supplementation increased the activities of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), and decreased malondialdehyde (MDA) levels in A. japonicus. Furthermore, the intestinal microbiota diversity of sea cucumbers was increased by dietary supplementation with κ-selenocarrageenan and the relative abundances of some probiotics (such as Sulfitobacter and Rhodobacteraceae) were also increased. It is suggested that κ-selenocarrageenan could increase the antioxidant capacity and modulate the intestinal microbiota of sea cucumbers A. japonicus. Further researches will be conducted for its optimal administration concentrations in vivo.

Tài liệu tham khảo

Wang JQ, Wang ZX, Zhang K, Zhang YM, Jiang YS, Liu CB, Yan-Qiang WU (2012) Effects of dietary selenomethionine levels on growth and some immune indices in juvenile sea cucumber Apostichopus japonicus. J Dalian Ocean Univ 27:110–115. https://doi.org/10.16535/j.cnki.dlhyxb.2012.02.009

Xiao-Qian LU, Zhang M, Yang YU, Wang ZF, Yang S, Dang ZQ, Liu JB, Zhou W (2015) Effects of dietary selenium methionine on feeding and growth in juvenile sea cucumber Apostichopus japonicus. Chin J Fish 4:18–23

Zhou W, Cao Q, Wang ZF, Xiao-Jie HU, Zhang JY, Liu JB (2015) Effect of dietary methionine selenium on growth and digestive index of sea cucumber Apostichopus japonicus. J Dalian Ocean Univ 30:181–184. https://doi.org/10.3969/J.ISSN.2095-1388.2015.02.013

Xiumei LI, Tao XU, Sun G, Yang J, Genrui LI, Haizhou LI (2017) Effects of dietary selenium-enriched yeast levels on important physiological enzymes and enrichment of selenium in sea cucumber (Apostichopus japonicus). Prog Fish Sci 38(4):155–163. https://doi.org/10.11758/yykxjz.20140512003

Levander OA (2000) The selenium-coxsackievirus connection: chronicle of a collaboration. J Nutr 130(2S Suppl):485S–488S. https://doi.org/10.1093/jn/130.2.485S