Biotechnology and Bioengineering
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Characterization of the mutant lytic state in lambda expression systems
Biotechnology and Bioengineering - Tập 39 Số 4 - Trang 369-377 - 1992
AbstractThe two propagative phases of bacteriophage lambda, lysogeny and lysis,
can be used in concert to enhance productivity of recombinant expression
systems. Lambda vectors carrying mutations to prevent both cell lysis and λ DNA
packaging in the lytic state have been shown to yield 100% stability of the
product gene in lysogeny and to produce up to 15% of total cell protein as
product β‐galact...
Effect of cycling on the stability of plasmid‐bearing microorganisms in continuous culture
Biotechnology and Bioengineering - Tập 31 Số 5 - Trang 464-469 - 1988
AbstractThe implication of the possible existence of differences in the times
required for plasmid‐bearing and non‐plasmid‐bearing microorganisms to adjust
their metabolic activities to step changes in their environment is examined.
This adaptability difference suggests the possibility of maintaining an
engineered strain in continuous culture by transient operation. It is shown for
the case where ...
Effect of operating parameters on specific production rate of a cloned‐gene product and performance of recombinant fermentation process
Biotechnology and Bioengineering - Tập 35 Số 3 - Trang 287-295 - 1990
AbstractA two‐stage continuous system in combination with a
temperature‐sensitive expression system were used as model systems to maximize
the productivity of a cloned gene and minimize the problem associated with the
plasmid instability for a high‐expression recombinant. In order to optimize the
two‐stage fermentation process, the effects of such operational variables as
temperature and dilution ...
Evaluation of the hok/sok killer locus for enhanced plasmid stability
Biotechnology and Bioengineering - Tập 44 Số 8 - Trang 912-921 - 1994
AbstractThe effectiveness of the hok/sok plasmid stability locus and mechanism
of cloned‐gene loss was evaluated in shake‐flask cultures. Addition of the
hok/sok locus dramitically increasedapparent plasmid segregational stability to
the hok/sok− control. In terms of the number of generations before 10%of the
population became plasmid‐free, segregational stability was increased by 11‐ to
20‐fold i...
Two‐stage fermentation with bacteriophage λ as an expression vector in Escherachia coli
Biotechnology and Bioengineering - Tập 37 Số 4 - Trang 297-302 - 1991
AbstractThe potential of bacteriophage λ as an expression vector for a large
scale production of cloned‐gene proteins was evaluated in batch and continuous
bioreactors using a temperature‐sensitive mutant in the cl gene, which allows a
simple manipulation of temperature as a means to control the phage in the
lysogenic or lytic state. A temperature switch from 32°C (or below) to 38°C (or
above) for...
The effect of controlled feeding of glycerol on β‐galactosidase production byEscherichia coliin batch culture
Biotechnology and Bioengineering - Tập 15 Số 6 - Trang 1179-1188 - 1973
AbstractA mutant ofE. coliconstitutive for β‐galactosidase has been grown in
batch culture with the carbon source, glycerol, fed at various fixed rates to
the culture. High feeding rates where growth was only slightly restricted gave
final enzyme levels similar to those obtained in cultures where all the glycerol
was added initially. Low feeding rates resulted in breakdown of the
β‐galactosidase f...
Enhanced electron transfer of different mediators for strictly opposite shifting of metabolism in Clostridium pasteurianum grown on glycerol in a new electrochemical bioreactor
Biotechnology and Bioengineering - Tập 116 Số 7 - Trang 1627-1643 - 2019
AbstractMicrobial electrosynthesis or electro‐fermentation in bioelectrochemical
systems (BES) have recently received much attention. Here, we demonstrate with
the glycerol metabolism by Clostridium pasteurianum that H 2 from in situ water
electrolysis, especially in combination with a redox mediator, provides a simple
and flexible way for shifting product selectivity and enhancing product yield i...
Electrochemical control of redox potential in batch cultures of Escherichia coli
Biotechnology and Bioengineering - Tập 27 Số 10 - Trang 1512-1515 - 1985
Metabolite profiling for analysis of yeast stress response during very high gravity ethanol fermentations
Biotechnology and Bioengineering - Tập 90 Số 6 - Trang 703-714 - 2005
AbstractA laboratory strain and an industrial strain of Saccharomyces cerevisiae
were grown at high substrate concentration, so‐called very high gravity (VHG)
fermentation. Simultaneous saccharification and fermentation (SSF) was applied
in a batch process using 280 g/L maltodextrin as carbon source. It was shown
that known ethanol and osmotic stress responses such as decreased growth rate,
lower ...
Opportunities for renewable bioenergy using microorganisms
Biotechnology and Bioengineering - Tập 100 Số 2 - Trang 203-212 - 2008
AbstractGlobal warming can be slowed, and perhaps reversed, only when society
replaces fossil fuels with renewable, carbon‐neutral alternatives. The best
option is bioenergy: the sun's energy is captured in biomass and converted to
energy forms useful to modern society. To make a dent in global warming,
bioenergy must be generated at a very high rate, since the world today uses ∼10
TW of fossil‐fu...
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