Biotechnology and Bioengineering

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Characterization of the mutant lytic state in lambda expression systems
Biotechnology and Bioengineering - Tập 39 Số 4 - Trang 369-377 - 1992
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe two propagative phases of bacteriophage lambda, lysogeny and lysis, can be used in concert to enhance productivity of recombinant expression systems. Lambda vectors carrying mutations to prevent both cell lysis and λ DNA packaging in the lytic state have been shown to yield 100% stability of the product gene in lysogeny and to produce up to 15% of total...... hiện toàn bộ
Effect of cycling on the stability of plasmid‐bearing microorganisms in continuous culture
Biotechnology and Bioengineering - Tập 31 Số 5 - Trang 464-469 - 1988
M. L. Stephens, G. Lyberatos
AbstractThe implication of the possible existence of differences in the times required for plasmid‐bearing and non‐plasmid‐bearing microorganisms to adjust their metabolic activities to step changes in their environment is examined. This adaptability difference suggests the possibility of maintaining an engineered strain in continuous culture by transient operation...... hiện toàn bộ
Effect of operating parameters on specific production rate of a cloned‐gene product and performance of recombinant fermentation process
Biotechnology and Bioengineering - Tập 35 Số 3 - Trang 287-295 - 1990
Taesun Park, Dewey D. Y. Ryu
AbstractA two‐stage continuous system in combination with a temperature‐sensitive expression system were used as model systems to maximize the productivity of a cloned gene and minimize the problem associated with the plasmid instability for a high‐expression recombinant. In order to optimize the two‐stage fermentation process, the effects of such operational varia...... hiện toàn bộ
Evaluation of the hok/sok killer locus for enhanced plasmid stability
Biotechnology and Bioengineering - Tập 44 Số 8 - Trang 912-921 - 1994
Kuo‐Wei Wu, Thomas K. Wood
AbstractThe effectiveness of the hok/sok plasmid stability locus and mechanism of cloned‐gene loss was evaluated in shake‐flask cultures. Addition of the hok/sok locus dramitically increasedapparent plasmid segregational stability to the hok/sok− control. In terms ...... hiện toàn bộ
Two‐stage fermentation with bacteriophage λ as an expression vector in Escherachia coli
Biotechnology and Bioengineering - Tập 37 Số 4 - Trang 297-302 - 1991
Tai Hyun Park, Jin‐Ho Seo, Henry C. Lim
AbstractThe potential of bacteriophage λ as an expression vector for a large scale production of cloned‐gene proteins was evaluated in batch and continuous bioreactors using a temperature‐sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or lytic state. A te...... hiện toàn bộ
The effect of controlled feeding of glycerol on β‐galactosidase production byEscherichia coliin batch culture
Biotechnology and Bioengineering - Tập 15 Số 6 - Trang 1179-1188 - 1973
Peter P. Gray, P. Dunnill, M. D. Lilly
AbstractA mutant ofE. coliconstitutive for β‐galactosidase has been grown in batch culture with the carbon source, glycerol, fed at various fixed rates to the culture. High feeding rates where growth was only slightly restricted gave final enzyme levels similar to those obtained in cultures where all the glycerol was added initially. Low ...... hiện toàn bộ
Enhanced electron transfer of different mediators for strictly opposite shifting of metabolism in Clostridium pasteurianum grown on glycerol in a new electrochemical bioreactor
Biotechnology and Bioengineering - Tập 116 Số 7 - Trang 1627-1643 - 2019
Tyll Utesch, Wael Sabra, Christin Prescher, Julian Baur, Philipp Arbter, An‐Ping Zeng
AbstractMicrobial electrosynthesis or electro‐fermentation in bioelectrochemical systems (BES) have recently received much attention. Here, we demonstrate with the glycerol metabolism by Clostridium pasteurianum that H 2 from in situ water electrolysis, especially in combination with a redox mediator, provides a simpl...... hiện toàn bộ
Electrochemical control of redox potential in batch cultures of Escherichia coli
Biotechnology and Bioengineering - Tập 27 Số 10 - Trang 1512-1515 - 1985
B. G. Thompson, Donald F. Gerson
Metabolite profiling for analysis of yeast stress response during very high gravity ethanol fermentations
Biotechnology and Bioengineering - Tập 90 Số 6 - Trang 703-714 - 2005
Rasmus Devantier, Britta Scheithauer, Silas G. Villas‐Bôas, Sven Pedersen, Lisbeth Olsson
AbstractA laboratory strain and an industrial strain of Saccharomyces cerevisiae were grown at high substrate concentration, so‐called very high gravity (VHG) fermentation. Simultaneous saccharification and fermentation (SSF) was applied in a batch process using 280 g/L maltodextrin as carbon source. It was shown that known ethanol and os...... hiện toàn bộ
Opportunities for renewable bioenergy using microorganisms
Biotechnology and Bioengineering - Tập 100 Số 2 - Trang 203-212 - 2008
Bruce E. Rittmann
AbstractGlobal warming can be slowed, and perhaps reversed, only when society replaces fossil fuels with renewable, carbon‐neutral alternatives. The best option is bioenergy: the sun's energy is captured in biomass and converted to energy forms useful to modern society. To make a dent in global warming, bioenergy must be generated at a very high rate, since the wor...... hiện toàn bộ
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