Characterization of the mutant lytic state in lambda expression systemsBiotechnology and Bioengineering - Tập 39 Số 4 - Trang 369-377 - 1992
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe two propagative phases of bacteriophage lambda, lysogeny and lysis, can be used in concert to enhance productivity of recombinant expression systems. Lambda vectors carrying mutations to prevent both cell lysis and λ DNA packaging in the lytic state have been shown to yield 100% stability of the product gene in lysogeny and to produce up to 15% of total...... hiện toàn bộ
Effect of cycling on the stability of plasmid‐bearing microorganisms in continuous cultureBiotechnology and Bioengineering - Tập 31 Số 5 - Trang 464-469 - 1988
M. L. Stephens, G. Lyberatos
AbstractThe implication of the possible existence of differences in the times required for plasmid‐bearing and non‐plasmid‐bearing microorganisms to adjust their metabolic activities to step changes in their environment is examined. This adaptability difference suggests the possibility of maintaining an engineered strain in continuous culture by transient operation...... hiện toàn bộ
Two‐stage fermentation with bacteriophage λ as an expression vector in Escherachia coliBiotechnology and Bioengineering - Tập 37 Số 4 - Trang 297-302 - 1991
Tai Hyun Park, Jin‐Ho Seo, Henry C. Lim
AbstractThe potential of bacteriophage λ as an expression vector for a large scale production of cloned‐gene proteins was evaluated in batch and continuous bioreactors using a temperature‐sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or lytic state. A te...... hiện toàn bộ
The effect of controlled feeding of glycerol on β‐galactosidase production byEscherichia coliin batch cultureBiotechnology and Bioengineering - Tập 15 Số 6 - Trang 1179-1188 - 1973
Peter P. Gray, P. Dunnill, M. D. Lilly
AbstractA mutant ofE. coliconstitutive for β‐galactosidase has been grown in batch culture with the carbon source, glycerol, fed at various fixed rates to the culture. High feeding rates where growth was only slightly restricted gave final enzyme levels similar to those obtained in cultures where all the glycerol was added initially. Low ...... hiện toàn bộ
Metabolite profiling for analysis of yeast stress response during very high gravity ethanol fermentationsBiotechnology and Bioengineering - Tập 90 Số 6 - Trang 703-714 - 2005
Rasmus Devantier, Britta Scheithauer, Silas G. Villas‐Bôas, Sven Pedersen, Lisbeth Olsson
AbstractA laboratory strain and an industrial strain of Saccharomyces cerevisiae were grown at high substrate concentration, so‐called very high gravity (VHG) fermentation. Simultaneous saccharification and fermentation (SSF) was applied in a batch process using 280 g/L maltodextrin as carbon source. It was shown that known ethanol and os...... hiện toàn bộ
Opportunities for renewable bioenergy using microorganismsBiotechnology and Bioengineering - Tập 100 Số 2 - Trang 203-212 - 2008
Bruce E. Rittmann
AbstractGlobal warming can be slowed, and perhaps reversed, only when society replaces fossil fuels with renewable, carbon‐neutral alternatives. The best option is bioenergy: the sun's energy is captured in biomass and converted to energy forms useful to modern society. To make a dent in global warming, bioenergy must be generated at a very high rate, since the wor...... hiện toàn bộ
Suspension culture of hepatocyte‐derived reporter cells in presence of albumin to form stable three‐dimensional spheroidsBiotechnology and Bioengineering - Tập 110 Số 9 - Trang 2548-2555 - 2013
Christopher Weeks, Kristen Newman, Paul A. Turner, Brian R. Rodysill, Raymond Hickey, Scott L. Nyberg, Amol V. Janorkar
AbstractSeveral studies in the past have formed 3‐dimensional (3D) spheroids of primary hepatocytes in suspension culture. Unfortunately, primary hepatocytes in a suspension environment tend to lose their differentiated function over time, generally due to damage from fluid shear stress and eventual spheroid settling. We have therefore creat...... hiện toàn bộ
Design and construction of improved new vectors for Zymomonas mobilis recombinantsBiotechnology and Bioengineering - Tập 108 Số 7 - Trang 1616-1627 - 2011
Hongwei Dong, Jie Bao, Dewey D. Y. Ryu, Jian‐Jiang Zhong
AbstractZymomonas mobilis is a very important gram‐negative bacterium having a potential application to simultaneous co‐production of biofuel and other high value‐added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. H...... hiện toàn bộ