Biotechnology and Bioengineering

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Characterization of the mutant lytic state in lambda expression systems
Biotechnology and Bioengineering - Tập 39 Số 4 - Trang 369-377 - 1992
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe two propagative phases of bacteriophage lambda, lysogeny and lysis, can be used in concert to enhance productivity of recombinant expression systems. Lambda vectors carrying mutations to prevent both cell lysis and λ DNA packaging in the lytic state have been shown to yield 100% stability of the product gene in lysogeny and to produce up to 15% of total...... hiện toàn bộ
Two‐stage fermentation with bacteriophage λ as an expression vector in Escherachia coli
Biotechnology and Bioengineering - Tập 37 Số 4 - Trang 297-302 - 1991
Tai Hyun Park, Jin‐Ho Seo, Henry C. Lim
AbstractThe potential of bacteriophage λ as an expression vector for a large scale production of cloned‐gene proteins was evaluated in batch and continuous bioreactors using a temperature‐sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or lytic state. A te...... hiện toàn bộ
The effect of controlled feeding of glycerol on β‐galactosidase production byEscherichia coliin batch culture
Biotechnology and Bioengineering - Tập 15 Số 6 - Trang 1179-1188 - 1973
Peter P. Gray, P. Dunnill, M. D. Lilly
AbstractA mutant ofE. coliconstitutive for β‐galactosidase has been grown in batch culture with the carbon source, glycerol, fed at various fixed rates to the culture. High feeding rates where growth was only slightly restricted gave final enzyme levels similar to those obtained in cultures where all the glycerol was added initially. Low ...... hiện toàn bộ
Metabolite profiling for analysis of yeast stress response during very high gravity ethanol fermentations
Biotechnology and Bioengineering - Tập 90 Số 6 - Trang 703-714 - 2005
Rasmus Devantier, Britta Scheithauer, Silas G. Villas‐Bôas, Sven Pedersen, Lisbeth Olsson
AbstractA laboratory strain and an industrial strain of Saccharomyces cerevisiae were grown at high substrate concentration, so‐called very high gravity (VHG) fermentation. Simultaneous saccharification and fermentation (SSF) was applied in a batch process using 280 g/L maltodextrin as carbon source. It was shown that known ethanol and os...... hiện toàn bộ
Opportunities for renewable bioenergy using microorganisms
Biotechnology and Bioengineering - Tập 100 Số 2 - Trang 203-212 - 2008
Bruce E. Rittmann
AbstractGlobal warming can be slowed, and perhaps reversed, only when society replaces fossil fuels with renewable, carbon‐neutral alternatives. The best option is bioenergy: the sun's energy is captured in biomass and converted to energy forms useful to modern society. To make a dent in global warming, bioenergy must be generated at a very high rate, since the wor...... hiện toàn bộ
Design and construction of improved new vectors for Zymomonas mobilis recombinants
Biotechnology and Bioengineering - Tập 108 Số 7 - Trang 1616-1627 - 2011
Hongwei Dong, Jie Bao, Dewey D. Y. Ryu, Jian‐Jiang Zhong
AbstractZymomonas mobilis is a very important gram‐negative bacterium having a potential application to simultaneous co‐production of biofuel and other high value‐added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. H...... hiện toàn bộ
Influence of the water activity of a solid substrate on the growth rate and sporogenesis of filamentous fungi
Biotechnology and Bioengineering - Tập 31 Số 5 - Trang 457-463 - 1988
P. Gervats, Paul Molin, W. Grajek, Maurice Bensoussan
AbstractThe water activity (aw) of substrates has been related to the mycelial growth and the sporogenesis of two molds. In the absence of other limiting factors, optimal aw values were determined for growth and sporo...... hiện toàn bộ
A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity
Biotechnology and Bioengineering - Tập 99 Số 1 - Trang 129-135 - 2008
Isabelle Meynial‐Salles, Sophie Dorotyn, Philippe Soucaille
AbstractA novel three stages continuous fermentation process for the bioproduction of succinic acid at high concentration, productivity and yield using A. succiniciproducens was developed. This process combined an integrated membrane‐bioreactor‐electrodialysis system. An energetic characterization of A. succiniciproducens... hiện toàn bộ
A novel BOD sensor based on bacterial luminescence
Biotechnology and Bioengineering - Tập 41 Số 11 - Trang 1107-1111 - 1993
Chang‐Kee Hyun, Eiichi Tamiya, Toshifumi Takeuchi, Isao Karube, Noriuki Inoue
AbstractA reagent‐type BOD sensor with a new principle employing a luminous bacterium, Photobacterium phosphoreum, was developed. The increased intensity of luminescence resulting from the cellular assimilation of organic compounds in wastewater was detected by a photodiode. The BOD response of the bacterial reagent could be obtained with...... hiện toàn bộ
Carboxylates and alcohols production in an autotrophic hydrogen‐based membrane biofilm reactor
Biotechnology and Bioengineering - Tập 118 Số 6 - Trang 2338-2347 - 2021
Diana C. Calvo, Aura Ontiveros‐Valencia, Rosa Krajmalnik‐Brown, César I. Torres, Bruce E. Rittmann
AbstractMicrobiological conversion of CO2 into biofuels and/or organic industrial feedstock is an excellent carbon‐cycling strategy. Here, autotrophic anaerobic bacteria in the membrane biofilm reactor (MBfR) transferred electrons from hydrogen gas (H2) to inorganic carbon (IC) and produced organic acids and alcohols. We sy...... hiện toàn bộ
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