The effect of controlled feeding of glycerol on β‐galactosidase production byEscherichia coliin batch culture

Biotechnology and Bioengineering - Tập 15 Số 6 - Trang 1179-1188 - 1973
Peter P. Gray1, P. Dunnill1, M. D. Lilly1
1Biochemical Engineering Section, Department of Chemical Enginering, University College London, London, England

Tóm tắt

AbstractA mutant ofE. coliconstitutive for β‐galactosidase has been grown in batch culture with the carbon source, glycerol, fed at various fixed rates to the culture. High feeding rates where growth was only slightly restricted gave final enzyme levels similar to those obtained in cultures where all the glycerol was added initially. Low feeding rates resulted in breakdown of the β‐galactosidase formed and gave reduced final levels of the enzyme.

Từ khóa


Tài liệu tham khảo

Terui G., 1967, J. Ferment. Technol., 45, 497

Kinoshita S., 1967, J. Ferment. Technol., 49, 504

Gray P. P., 1972, Fermentation Technology Today, 347

Demain A. L., 1971, Methods in Enzymology, 86

Pardee A. B., 1969, Fermentation Advances, 3

Gray P. P., 1972, J. Ferment. Technol., 50, 381

10.1021/ie50414a005

10.1002/bit.260110403

Phares E. F., 1971, Methods in Enzymology, 445

Elsworth R., 1968, J. Appl. Chem., 17, 157, 10.1002/jctb.5010180601

Fujio Y., 1971, J. Ferment. Technol., 49, 626

Fujio Y., 1972, J. Ferment. Technol., 50, 546

10.1093/oxfordjournals.jbchem.a129238

10.1146/annurev.mi.26.100172.000535

10.1016/S0021-9258(19)45938-3

10.1038/2281151a0

Thông tin
Thông tin xuất bản

Biotechnology and Bioengineering

Tập 15 Số 6

1179-1188

Thông tin tác giả