Improvement of ethanol productivity and energy efficiency by degradation of inhibitors using recombinant Zymomonas mobilis (pHW20a‐fdh)

Biotechnology and Bioengineering - Tập 110 Số 9 - Trang 2395-2404 - 2013
Hongwei Dong1,2, Liqiang Fan2, Zichen Luo2, Jian‐Jiang Zhong3, Dewey D. Y. Ryu4, Jie Bao2
1Shanghai Research Institute of Chemical Industry, Shanghai, China
2State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
3State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China
4Biochemical Engineering Program University of California One Shields Avenue Davis California 95616

Tóm tắt

AbstractToxic compounds, such as formic acid, furfural, and hydroxymethylfurfural (HMF) generated during pretreatment of corn stover (CS) at high temperature and low pH, inhibit growth of Zymomonas mobilis and lower the conversion efficiency of CS to biofuel and other products. The inhibition of toxic compounds is considered as one of the major technical barriers in the lignocellulose bioconversion. In order to detoxify and/or degrade these toxic compounds by the model ethanologenic strain Z. mobilis itself in situ the fermentation medium, we constructed a recombinant Z. mobilis ZM4 (pHW20a‐fdh) strain that is capable of degrading toxic inhibitor, formate. This is accomplished by cloning heterologous formate dehydrogenase gene (fdh) from Saccharomyces cerevisiae and by coupling this reaction of NADH regeneration reaction system with furfural and HMF degradation in the recombinant Z. mobilis strain. The NADH regeneration reaction also improved both the energy efficiency and cell physiological activity of the recombinant organism, which were definitely confirmed by the improved cell growth, ethanol yield, and ethanol productivity during fermentation with CS hydrolysate. Biotechnol. Bioeng. 2013; 110:2395–2404. © 2013 Wiley Periodicals, Inc.

Từ khóa


Tài liệu tham khảo

10.1186/1754-6834-1-12

10.1385/ABAB:134:1:15

10.1016/0003-2697(73)90094-8

10.1006/mben.2002.0227

10.1128/jb.169.6.2591-2597.1987

10.1016/0141-0229(95)00237-5

10.1002/bit.23106

Gorsich S, 2006, Tolerance to furfural‐induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiae, Appl Biochem Biotechnol, 71, 339

10.1073/pnas.87.12.4645

10.1023/A:1005320306410

10.1016/j.febslet.2006.08.034

Klinke HB, 2004, Inhibition of ethanol‐producing yeast and bacteria by degradation products produced during pre‐treatment of biomass, Appl Biochem Biotechnol, 66, 10

10.1073/pnas.0913039107

Leonardo MR, 1996, Role of NAD in regulating the adhE gene of Escherichia coli, J Bacteriol, 178, 6013, 10.1128/jb.178.20.6013-6018.1996

Liang L, 2011, Effect of overexpression of malate dehydrogenase on succinic acid production in Escherichia coli NZN111, Sheng Wu Gong Cheng Xue Bao, 27, 1005

10.1007/s00253-011-3167-9

10.1002/yea.1370

10.1021/bp050115c

San K‐Y, 2002, Metabolic engineering through cofactor manipulation and its effects on metabolic flux redistribution in Escherichia coli, Metab Eng, 4, 182, 10.1006/mben.2001.0220

10.1038/nbt1183-784

10.1007/s10541-005-0071-x

10.1073/pnas.0914506107

10.1186/1471-2180-10-135

Yao S, 2010, Metabolic engineering to improve ethanol production in Thermoanaerobacter mathranii, Appl Biochem Biotechnol, 88, 199

10.1002/(SICI)1097-0290(20000605)68:5<524::AID-BIT6>3.0.CO;2-T

10.1016/j.biortech.2011.01.005

Thông tin
Thông tin xuất bản

Biotechnology and Bioengineering

Tập 110 Số 9

2395-2404

Thông tin tác giả