Metabolite profiling for analysis of yeast stress response during very high gravity ethanol fermentations

Biotechnology and Bioengineering - Tập 90 Số 6 - Trang 703-714 - 2005
Rasmus Devantier1, Britta Scheithauer, Silas G. Villas‐Bôas, Sven Pedersen, Lisbeth Olsson
1Starch, Applied Discovery, Research & Development, Novozymes A/S, Laurentsvej 51-53, DK-2880 Bagsvaerd, Denmark.

Tóm tắt

AbstractA laboratory strain and an industrial strain of Saccharomyces cerevisiae were grown at high substrate concentration, so‐called very high gravity (VHG) fermentation. Simultaneous saccharification and fermentation (SSF) was applied in a batch process using 280 g/L maltodextrin as carbon source. It was shown that known ethanol and osmotic stress responses such as decreased growth rate, lower viability, higher energy consumption, and intracellular trehalose accumulation occur in VHG SSF for both strains when compared with standard laboratory medium (20 g/L glucose). The laboratory strain was the most affected. GC‐MS metabolite profiling was applied for assessing the yeast stress response influence on cellular metabolism. It was found that metabolite profiles originating from different strains and/or fermentation conditions were unique and could be distinguished with the help of multivariate data analysis. Several differences in the metabolic responses to stressing conditions were revealed, particularly the increased energy consumption of stressed cells was also reflected in increased intracellular concentrations of pyruvate and related metabolites. © 2005 Wiley Periodicals, Inc.

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Thông tin xuất bản

Biotechnology and Bioengineering

Tập 90 Số 6

703-714

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