A novel BOD sensor based on bacterial luminescence

Biotechnology and Bioengineering - Tập 41 Số 11 - Trang 1107-1111 - 1993
Chang‐Kee Hyun1, Eiichi Tamiya1, Toshifumi Takeuchi1, Isao Karube1, Noriuki Inoue2
1Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153, Japan
2Heuristic Japan, Inc., 3‐28‐6 Yoyogi, shibuya‐ku, Tokyo 151, Japan

Tóm tắt

AbstractA reagent‐type BOD sensor with a new principle employing a luminous bacterium, Photobacterium phosphoreum, was developed. The increased intensity of luminescence resulting from the cellular assimilation of organic compounds in wastewater was detected by a photodiode. The BOD response of the bacterial reagent could be obtained within 15 min with ±7% error. The temperature condition for optimal BOD response was 18° to 25°C at pH 7 to 8, indicating that it is possible to measure BOD at room temperature without having to stabilize the temperature of the measuring system. For practical use, two procedures for long‐term preservation of the bacterial reagent, vacuum drying method and freezing method, are suggested. The metabolic characteristics of employed luminous bacteria were investigated by comparing the BOD values for several pure organic substrates estimated by the BOD sensor with conventional 5‐day BOD values. In comparison with the 5‐day measurement for some wastewater samples, BOD values estimated by the sensor showed comparatively good agreement with those measured by the 5‐day method. © 1993 Wiley & Sons, Inc.

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Thông tin xuất bản

Biotechnology and Bioengineering

Tập 41 Số 11

1107-1111

Thông tin tác giả