Two‐stage fermentation with bacteriophage λ as an expression vector in Escherachia coli

Biotechnology and Bioengineering - Tập 37 Số 4 - Trang 297-302 - 1991
Tai Hyun Park1, Jin‐Ho Seo1, Henry C. Lim2
1School of Chemical Engineering, Purdue University, West Lafayette, Indiana, 47907
2Biochemical Engineering Program, School of Engineering, University of California, Irvine, California 92717

Tóm tắt

AbstractThe potential of bacteriophage λ as an expression vector for a large scale production of cloned‐gene proteins was evaluated in batch and continuous bioreactors using a temperature‐sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or lytic state. A temperature switch from 32°C (or below) to 38°C (or above) forces the phage to go from the lysogenic state to the lytic state. Temperature cycling and a two‐reactor system were used for continuous cultures. For the latter the first reactor is maintained in the lysogenic state at a lower temperature to stably maintain the foreign DNA in the host cell, while the second reactor is maintained in the lytic state to force replication of the cloned‐gene and overproduction of its products. The results are promising but suggest a greater potential for a mutant which lacks the Q gene which is responsible for host cell lysis and packaging of phage particles.

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Thông tin xuất bản

Biotechnology and Bioengineering

Tập 37 Số 4

297-302

Thông tin tác giả