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Các bài báo tiêu biểu
Cleavage of Zearalenone‐Glycoside, a “Masked” Mycotoxin, during Digestion in Swine
Tập 37 Số 1-10 - Trang 236-240 - 1990
SummaryComparative analyses of cereal samples pretreated with or without
β‐glucosidase indicate the presence of zearalenone‐glycoside. To examine the
stability of zearalenone‐glycoside during digestion, mixed feed was artificially
contaminated with synthesized zearalenone‐4‐β‐D‐glucopyranoside (395 μg/kg) and
fed to a pig over a period of 14 days. The metabolites detected in feces and
urine sample...
The Use of Monoclonal Antibodies in Enzyme Linked Immunosorbent Assays for Detection of Antibodies to Bovine Viral Diarrhoea Virus
Tập 34 Số 1-10 - Trang 356-363 - 1987
SummaryTwo ELISA techniques were developed for the detection of antibodies to
bovine viral diarrhoea virus (BVDV), an indirect and a competitive ELISA. The
coating antigen was prepared from virus harvests which were 10 to 100 times
larger than usual. This was achieved by culturing the cells in a growth medium
with foetal calf serum devoid of IgG. The harvested BVDV was solubilized with
detergent t...
The Effect of Climate on the Presence of Culicoides imicola in Italy
Tập 50 Số 3 - Trang 139-147 - 2003
SummaryA model was developed to classify the Italian territories in relation to
their suitability to harbour populations of Culicoides imicola and, as a
consequence, also able to sustain a bluetongue (BT) epidemic. Italy was
subdivided into 3507 10 × 10 km cells. In 546 cells at least one collection was
made. The cell was considered the unit for all subsequent analyses. Culicoides
were collected u...
Evaluation of an Enzyme‐Linked Immunosorbent Assay for Detection of Antibodies to Bovine Virus Diarrhoea Virus in Milk
Tập 36 Số 1-10 - Trang 113-118 - 1989
SummaryThe present study shows that milk is an appropriate source for detection
of seroreactors to bovine virus diarrhoea virus (BVDV). There was close
agreement between antibody titres in serum and in skim milk, as determined by an
indirect enzyme‐linked immunosorbent assay. The antibody titres were usually
lower in skim milk than in serum, but all seropositive cows (n = 84) were also
skim milk‐p...
Phylogenetic Analysis of the 5′‐Untranslated Region of German BVDV Type II Isolates
Tập 49 Số 1 - Trang 43-47 - 2002
On the basis of genetic differences, bovine viral diarrhoea viruses (BVDV) are
subclassified into two distinct genotypes, BVDV type I and BVDV type II. We
selected German BVDV type II isolates using the BVDV type I‐specific monoclonal
antibody WB160 and flow cytometric analysis for further characterization. For
molecular characterization, a 288‐bp fragment of the 5′‐untranslated region
(5′‐UTR) of...
Oncolytic Potential of Rodent Parvoviruses for Cancer Therapy in Humans: A Brief Review
Tập 52 Số 7-8 - Trang 327-330 - 2005
SummaryRodent parvoviruses are promising candidates for oncolytic virotherapy of
cancer in humans because of their oncotropism (preferential killing of
transformed cells) in the absence of pathogenicity. Here, we give an overview
concerning the possible application of parvovirus H‐1 for cancer therapy, with
specific emphasis on malignant brain tumours in humans.
Differentiation of Salmonella Gallinarum biovar Gallinarum from Salmonella Gallinarum biovar Pullorum by PCR‐RFLP of the fimH gene
Tập 52 Số 5 - Trang 214-218 - 2005
SummaryIn our studies on FimH adhesins expressed by different Salmonella
serovars, we cloned and sequenced the fimH genes from Salmonella enterica ssp.
Enterica ser. Gallinarum biovar Gallinarum and S. enterica ssp. Enterica ser.
Gallinarum biovar Pullorum. Comparison of the nucleotide sequences revealed the
presence of a single‐nucleotide polymorphism (SNP) at position 544 bp from the A
of the st...
Investigations into the Prevention of Prenatal and Lactogenic Toxocara canis Infections in Puppies by Application of Moxidectin to the Pregnant Dog
Tập 53 Số 5 - Trang 218-223 - 2006
SummaryAim of the investigation was to examine whether two administrations of
moxidectin to pregnant dogs could prevent pre‐natal and lactogenic infections of
puppies with reactivated Toxocara canis larvae. Four pregnant beagles, infected
experimentally with 20 000 embryonated eggs of T. canis, were treated
subcutaneously with 1 mg moxidectin per kg body weight on days 40 and 55 of
pregnancy (5–13...
Distribution and Abundance of Bluetongue Vectors in Sardinia: Comparison of Field Data with Prediction Maps
Tập 53 Số 7 - Trang 312-316 - 2006
SummaryFollowing the spread of Bluetongue virus (BTV) in many Mediterranean
countries during the last 5 years, presence of the main BTV vector, Culicoides
imicola Kieffer (Diptera: Ceratopogonidae), was recorded in the region,
including the island of Sardinia where the first BT epidemic originally started
in the year 2000. Several models were also designed based on climate variables
and satellite ...