Phylogenetic Analysis of the 5′‐Untranslated Region of German BVDV Type II Isolates

Wiley - Tập 49 Số 1 - Trang 43-47 - 2002
Martin Beer1, Georg Wolf2, Oskar‐Rüger Kaaden2
1Institute for Medical Microbiology, Epidemic and Infectious Diseases, Veterinary Faculty, Munich, Germany.
2Institute for Medical Microbiology, Epidemic and Infectious Diseases, Veterinary Faculty, Veterinärstrasse 13, 80539 Munich, Germany

Tóm tắt

On the basis of genetic differences, bovine viral diarrhoea viruses (BVDV) are subclassified into two distinct genotypes, BVDV type I and BVDV type II. We selected German BVDV type II isolates using the BVDV type I‐specific monoclonal antibody WB160 and flow cytometric analysis for further characterization. For molecular characterization, a 288‐bp fragment of the 5′‐untranslated region (5′‐UTR) of the selected isolates was investigated by reverse transcription–polymerase chain reaction (RT‐PCR) and sequencing. Sequence comparisons of the partial 5′‐UTR sequences and their phylogenetic analyses demonstrated that the 18 German BVDV type II isolates all belong to either subtype IIa (10 isolates) or subtype IIc (eight isolates). Nevertheless, the German BVDV type II isolates were genetically different (89.9–94.3% sequence identity) from the standard BVDV type II strain 890 from North America, which was recently classified as BVDV type IIa. Furthermore, a clear subdivision of the German BVDV type II isolates into two distinct subtypes (BVDV IIa Germany and BVDV IIc Germany) is shown. Viruses of both subgroups differed in the analysed 5′‐UTR fragment from each other (91.6–95.2% sequence identity), but were highly conserved within the same German subtype (97.2–100% sequence identity). These findings are discussed in the context of BVDV type II origin, possible introduction into Germany, its epidemiology and impact for vaccine development.

Từ khóa


Tài liệu tham khảo

10.1016/S0749-0720(15)30460-6

10.1016/S0168-1702(97)00119-6

10.1099/0022-1317-78-6-1357

10.1006/viro.1999.9872

Beer M., 1999, Selection of BVDV genotype II isolates using a monoclonal antibody and FACS analysis, Berl. Münch. Tierarztl. Wochenschr., 112, 345

10.1016/S0378-1135(97)00145-4

Bielefeldt O. H., 1995, The pathologies of bovine viral diarrhea virus infection. A window on the pathogenesis, Vet. Clin. North Am. Food Anim. Pract., 11, 447, 10.1016/S0749-0720(15)30461-8

10.20506/rst.9.1.491

10.1016/0042-6822(88)90672-1

10.1111/j.1348-0421.1997.tb01936.x

10.1053/tvjl.2000.0500

10.1016/S0166-0934(97)00180-8

10.1016/S0378-1135(98)00262-4

10.1016/S0749-0720(15)30462-X

Nagai M., 1998, Nucleotide sequence homology to bovine viral diarrhea virus 2 (BVDV 2) in the 5′‐untranslated region of BVDVs from cattle with mucosal disease or persistent infection Japan, Vet. Microbiol., 60, 271, 10.1016/S0378-1135(98)00158-8

Page R. D. M., 1996, TREEVIEW: an application to display phylogenetic trees on personal computers, Comput. Appl. Biosci., 12, 357

10.1006/viro.1994.1483

10.1016/0378-1135(94)00117-F

Pfeffer M., 2000, A universal `one‐tube' RT‐PCR protocol for amplifying isolates of bovine viral diarrhoea virus, Vet. Res. Commun., 24, 491, 10.1023/A:1006407807133

10.1006/mcpr.1998.0158

10.1006/viro.1994.1620

10.1126/science.288.5463.55d

10.1093/oxfordjournals.molbev.a025664

10.1073/pnas.94.13.6815

10.1016/S0168-1702(01)00244-1

Van Regenmortel M. H. V. C. M.Fauquet D. H. L.Bishop E. B.Carstens M. K.Estes S. M.Lemon J.Maniloff M. A.Mayo D. J.McGeoch C. R.Pringle andR. B.Wickner 2000: Virus Taxonomy: VIIth Report of the ICTV pp. 1167. Academic Press SanDiego.

10.1006/viro.1997.8792

10.1007/BF01321060

10.1099/0022-1317-78-4-725

10.1016/S0378-1135(99)00111-X

10.1007/s007050050222

Thông tin
Thông tin xuất bản

Wiley

Tập 49 Số 1

43-47

Thông tin tác giả