Wiley
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Tetracycline inhibition identifies the cellular origin of interstitial collagenases in human periodontal diseases in vivoMammalian interstitial collagenases (E.C.3.4.24.7) are considered as key initiators of collagen degradation in periodontal diseases. However, the cellular sources of collagenases present in gingival crevicular fluid have not been completely clarified. Resident fibroblasts and epithelial cells as well as infiltrating neutrophils and monocyte/macrophages are potential sources of the enzymes.... ... hiện toàn bộ
Wiley - Tập 7 Số 2 - Trang 121-123 - 1992
Antimicrobial susceptibility of major pathogens of orofacial odontogenic infections to 11 β‐lactam antibioticsIn this study, we evaluated the current effectiveness of 11 β‐lactam antibiotics for treatment of orofacial odontogenic infections by determining the antimicrobial susceptibility of the major pathogens. The antimicrobial susceptibilities of viridans streptococci (n = 47), Peptostreptococcus (n = 67), ... hiện toàn bộ
Wiley - Tập 17 Số 5 - Trang 285-289 - 2002
Caries‐related plaque microcosm biofilms developed in microplates In vivo dental plaque biofilms consist of complex communities of oral bacteria that are a challenge to replicate in vitro . The aim of this investigation was to establish human dental plaque microcosms in microplates to reflect conditions that are relevant to dental caries. Microcosm plaque biofilms were initiated from the saliva of two... ... hiện toàn bộ
Wiley - Tập 22 Số 2 - Trang 73-79 - 2007
Simultaneous monitoring of intracellular pH and proton excretion during glycolysis by Streptococcus mutans and Streptococcus sanguis: effect of low pH and fluorideA system was developed by which 2′,7′‐bis(carboxyethyl)‐4 or 5‐carboxyfluorescein could be used to monitor intracellular pH at the same time that proton excretion was being measured. Streptococcal cells were loaded with the dye, and after the addition of glucose protons were excreted and the intracellular pH increased quickly and remained higher than the extracellular pH of 7.0. The excret... ... hiện toàn bộ
Wiley - Tập 10 Số 6 - Trang 355-359 - 1995
Protective and destructive immune reactions in apical periodontitisApical periodontitis is initiated primarily by the mixed microflora of infected root canals. Continuous flow of bacteria and their products through the apical foramen induces influx, activation and coordinated interaction of immune‐inflammatory cells within the periapical area. Successful mobilization of host defense mechanisms prevents abundant extraradicular bacterial invasion. However, ... ... hiện toàn bộ
Wiley - Tập 15 Số 3 - Trang 139-150 - 2000
Improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definitionBackground: Terminal restriction fragment length polymorphism (T‐RFLP) analysis is commonly used to analyze microbial communities, including oral microflora. However, accurate identification of terminal restriction fragment (T‐RF) origins is prevented by unpredictable errors in sizing, thus necessitating the clone library analysis. To minimize sizing errors, we prop...
Wiley - Tập 22 Số 6 - Trang 419-428 - 2007
Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitroIntroduction: The use of probiotic bacteria is increasing worldwide and at least some of them can transiently colonize the oral cavity. Several studies have shown that probiotic bacteria, which are often thought of in relation only to intestinal health, can also affect the oral ecology, but the mechanisms for this are largely unknown. The aim of this study was to in...
Wiley - Tập 23 Số 4 - Trang 336-343 - 2008
Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactionsA replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with 125 I‐amylase or ... hiện toàn bộ
Wiley - Tập 7 Số 1 - Trang 53-56 - 1992
Adherence to and penetration through endothelial cells by oral treponemesOral spirochetes were co‐incubated with monolayers of endothelial cells seeded into multiwell plates or onto filters mounted in plastic chambers. Attachment was assessed using an enzyme‐linked immunosorbent assay and scanning electron microscopy. Invasiveness was determined by monitoring media beneath filters within chambers for spirochetes using darkfield microscopy. Transmission electron... ... hiện toàn bộ
Wiley - Tập 14 Số 6 - Trang 379-383 - 1999
Differential gene expression of human β‐defensins (hBD‐1, ‐2, ‐3) in inflammatory gingival diseasesAntimicrobial peptides, like human β‐defensins, play an important role in the epithelial innate defense response. The aim of the present study was to investigate the quantitative expression of human β‐defensin‐1, ‐2, and ‐3 in inflammatory gingival diseases. Gingival biopsies were obtained from patients with healthy gingiva (n = 10), patients with gingivitis (... hiện toàn bộ
Wiley - Tập 20 Số 3 - Trang 186-190 - 2005
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