Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactionsWiley - Tập 7 Số 1 - Trang 53-56 - 1992
Chien‐Chi Tseng, Frank A. Scannapieco, Michael J. Levine
A replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with 125I‐amylase or ... hiện toàn bộ
Prevalence of mutans streptococci in one‐year‐old childrenWiley - Tập 6 Số 5 - Trang 280-283 - 1991
Margaret Grindefjord, Göran Dahllöf, S Wikner, Bengt Höjer, Thomas Modéer
Colonization with mutans streptococci was studied in 1095 1‐year‐old children living in suburban Stockholm. During a scheduled vaccination appointment at a child health centre, a bacterial sample was obtained from the child's tongue and a structured questionnaire was completed by the accompanying parent. Six percent of the subjects were colonized with mutans streptococci. The variables mos...... hiện toàn bộ
Tetracycline inhibition identifies the cellular origin of interstitial collagenases in human periodontal diseases in vivoWiley - Tập 7 Số 2 - Trang 121-123 - 1992
Kimmo Suomalainen, Timo Sorsa, Tuula Ingman, Otso Lindy, Lorne M. Golub
Mammalian interstitial collagenases (E.C.3.4.24.7) are considered as key initiators of collagen degradation in periodontal diseases. However, the cellular sources of collagenases present in gingival crevicular fluid have not been completely clarified. Resident fibroblasts and epithelial cells as well as infiltrating neutrophils and monocyte/macrophages are potential sources of the enzymes....... hiện toàn bộ
Adherence to and penetration through endothelial cells by oral treponemesWiley - Tập 14 Số 6 - Trang 379-383 - 1999
Sonja Peters, M Valdez, George R. Riviere, D D Thomas
Oral spirochetes were co‐incubated with monolayers of endothelial cells seeded into multiwell plates or onto filters mounted in plastic chambers. Attachment was assessed using an enzyme‐linked immunosorbent assay and scanning electron microscopy. Invasiveness was determined by monitoring media beneath filters within chambers for spirochetes using darkfield microscopy. Transmission electron...... hiện toàn bộ
Antimicrobial susceptibility of major pathogens of orofacial odontogenic infections to 11 β‐lactam antibioticsWiley - Tập 17 Số 5 - Trang 285-289 - 2002
Tomoari Kuriyama, Tadahiro Karasawa, Kiyomasa Nakagawa, Shinichi Nakamura, Erika Yamamoto
In this study, we evaluated the current effectiveness of 11 β‐lactam antibiotics for treatment of orofacial odontogenic infections by determining the antimicrobial susceptibility of the major pathogens. The antimicrobial susceptibilities of viridans streptococci (n = 47), Peptostreptococcus (n = 67), ... hiện toàn bộ
Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitroWiley - Tập 23 Số 4 - Trang 336-343 - 2008
Anna Haukioja, Vuokko Loimaranta, Jorma Tenovuo
Introduction: The use of probiotic bacteria is increasing worldwide and at least some of them can transiently colonize the oral cavity. Several studies have shown that probiotic bacteria, which are often thought of in relation only to intestinal health, can also affect the oral ecology, but the mechanisms for this are largely unknown. The aim of this study was to in...... hiện toàn bộ
Differential gene expression of human β‐defensins (hBD‐1, ‐2, ‐3) in inflammatory gingival diseasesWiley - Tập 20 Số 3 - Trang 186-190 - 2005
Henrik Dommisch, Yahya Açil, Anton Dunsche, Jochen Winter, Søren Jepsen
Antimicrobial peptides, like human β‐defensins, play an important role in the epithelial innate defense response. The aim of the present study was to investigate the quantitative expression of human β‐defensin‐1, ‐2, and ‐3 in inflammatory gingival diseases. Gingival biopsies were obtained from patients with healthy gingiva (n = 10), patients with gingivitis (... hiện toàn bộ
Protective and destructive immune reactions in apical periodontitisWiley - Tập 15 Số 3 - Trang 139-150 - 2000
Ildikó Márton, Csongor Kiss
Apical periodontitis is initiated primarily by the mixed microflora of infected root canals. Continuous flow of bacteria and their products through the apical foramen induces influx, activation and coordinated interaction of immune‐inflammatory cells within the periapical area. Successful mobilization of host defense mechanisms prevents abundant extraradicular bacterial invasion. However, ...... hiện toàn bộ