Wiley
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Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactions
Wiley - Tập 7 Số 1 - Trang 53-56 - 1992
A replica‐plate assay was used to screen for the interaction of salivary
molecules with dental plaque bacteria. Bacterial colonies cultured from
supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto
nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with
attached colonies were removed and incubated with 125I‐amylase or 125I
‐proline‐rich glycoprotein (PRG)....
Use of polymerase chain reaction techniques and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis for differentiation of oral Lactobacillus species
Wiley - Tập 21 Số 2 - Trang 79-83 - 2006
Background/aims: The genus Lactobacillus has been associated with dental caries
in humans, although it is seldom speciated due to lack of simple and
nonlaborious identification methods. A considerable heterogeneity among
Lactobacillus species has been demonstrated. The purpose of this study was to
develop simple methods combining restriction fragment length polymorphism
analysis of polymerase cha...
Prevalence of mutans streptococci in one‐year‐old children
Wiley - Tập 6 Số 5 - Trang 280-283 - 1991
Colonization with mutans streptococci was studied in 1095 1‐year‐old children
living in suburban Stockholm. During a scheduled vaccination appointment at a
child health centre, a bacterial sample was obtained from the child's tongue and
a structured questionnaire was completed by the accompanying parent. Six percent
of the subjects were colonized with mutans streptococci. The variables most
strong...
Tetracycline inhibition identifies the cellular origin of interstitial collagenases in human periodontal diseases in vivo
Wiley - Tập 7 Số 2 - Trang 121-123 - 1992
Mammalian interstitial collagenases (E.C.3.4.24.7) are considered as key
initiators of collagen degradation in periodontal diseases. However, the
cellular sources of collagenases present in gingival crevicular fluid have not
been completely clarified. Resident fibroblasts and epithelial cells as well as
infiltrating neutrophils and monocyte/macrophages are potential sources of the
enzymes. We have...
Adherence to and penetration through endothelial cells by oral treponemes
Wiley - Tập 14 Số 6 - Trang 379-383 - 1999
Oral spirochetes were co‐incubated with monolayers of endothelial cells seeded
into multiwell plates or onto filters mounted in plastic chambers. Attachment
was assessed using an enzyme‐linked immunosorbent assay and scanning electron
microscopy. Invasiveness was determined by monitoring media beneath filters
within chambers for spirochetes using darkfield microscopy. Transmission
electron microsc...
Antimicrobial susceptibility of major pathogens of orofacial odontogenic infections to 11 β‐lactam antibiotics
Wiley - Tập 17 Số 5 - Trang 285-289 - 2002
In this study, we evaluated the current effectiveness of 11 β‐lactam antibiotics
for treatment of orofacial odontogenic infections by determining the
antimicrobial susceptibility of the major pathogens. The antimicrobial
susceptibilities of viridans streptococci (n = 47), Peptostreptococcus (n = 67),
Porphyromonas (n = 18), Fusobacterium (n = 57), black‐pigmented Prevotella
(n = 59) and non‐pigmen...
Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro
Wiley - Tập 23 Số 4 - Trang 336-343 - 2008
Introduction: The use of probiotic bacteria is increasing worldwide and at
least some of them can transiently colonize the oral cavity. Several studies
have shown that probiotic bacteria, which are often thought of in relation only
to intestinal health, can also affect the oral ecology, but the mechanisms for
this are largely unknown. The aim of this study was to investigate in vitro if
the probi...
Improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition
Wiley - Tập 22 Số 6 - Trang 419-428 - 2007
Background: Terminal restriction fragment length polymorphism (T‐RFLP) analysis
is commonly used to analyze microbial communities, including oral microflora.
However, accurate identification of terminal restriction fragment (T‐RF) origins
is prevented by unpredictable errors in sizing, thus necessitating the clone
library analysis. To minimize sizing errors, we proposed optimizing the size
defini...
Differential gene expression of human β‐defensins (hBD‐1, ‐2, ‐3) in inflammatory gingival diseases
Wiley - Tập 20 Số 3 - Trang 186-190 - 2005
Antimicrobial peptides, like human β‐defensins, play an important role in the
epithelial innate defense response. The aim of the present study was to
investigate the quantitative expression of human β‐defensin‐1, ‐2, and ‐3 in
inflammatory gingival diseases. Gingival biopsies were obtained from patients
with healthy gingiva (n = 10), patients with gingivitis (n = 10), and patients
with periodontit...
Protective and destructive immune reactions in apical periodontitis
Wiley - Tập 15 Số 3 - Trang 139-150 - 2000
Apical periodontitis is initiated primarily by the mixed microflora of infected
root canals. Continuous flow of bacteria and their products through the apical
foramen induces influx, activation and coordinated interaction of
immune‐inflammatory cells within the periapical area. Successful mobilization of
host defense mechanisms prevents abundant extraradicular bacterial invasion.
However, anti‐inf...
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