Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactions

Wiley - Tập 7 Số 1 - Trang 53-56 - 1992
Chien‐Chi Tseng1, Frank A. Scannapieco1, Michael J. Levine1
1Department of Oral Biology and Dental Research Institute, School of Dental Medicine, State University of New York at Buffalo, USA

Tóm tắt

A replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with 125I‐amylase or 125I ‐proline‐rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase, and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.

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Wiley

Tập 7 Số 1

53-56

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