Tetracycline inhibition identifies the cellular origin of interstitial collagenases in human periodontal diseases in vivo

Wiley - Tập 7 Số 2 - Trang 121-123 - 1992
Kimmo Suomalainen1, Timo Sorsa1, Tuula Ingman1, Otso Lindy1, Lorne M. Golub2
1Department of Periodontology and Medical Chemistry, University of Helsinki, Finland
2Department of Oral Biology and Pathology, State University of New York at Stony Brook, USA.

Tóm tắt

Mammalian interstitial collagenases (E.C.3.4.24.7) are considered as key initiators of collagen degradation in periodontal diseases. However, the cellular sources of collagenases present in gingival crevicular fluid have not been completely clarified. Resident fibroblasts and epithelial cells as well as infiltrating neutrophils and monocyte/macrophages are potential sources of the enzymes. We have recently found significant differences in tetracycline inhibition between human neulrophil and fibroblast interstitial collagenases. To address the cellular source of collagenase present in gingival crevicular fluid in 2 distinct periodontal diseases, we studied the tetracycline inhibition of collagenase in gingival crevicular fluid of patients with localized juvenile periodontitis and adult periodontitis. Gingival crevicular fluid samples were collected from deep (<5 mm) periodontal pockets and assayed for collagenase in the presence of 0‐1000 μM doxycycline as well as a chemically modified tetracycline devoid of antimicrobial activity (4‐de‐dimethylaminotelracycline). The drug concentration required to inhibit 50% of collagenase activity (IC50) in localized juvenile periodontitis gingival crevicular fluid was 280 μM for doxycycline and 470 μM for 4‐de‐dimethylaminotetracycline. Significantly lower values, 10–20 μM, were obtained for collagenase in gingival crevicular fluid of patients with adult periodontitis. We propose that systemic letracycline levels are efficient inhibitors of collagenase in gingival crevicular fluid in affected sites of patients with adult periodontitis but not of patients with localized juvenile periodontitis and that the fibroblast type interstitial collagenase is the predominant collagenase type in gingival crevicular fluid in affected sites of patients with localized juvenile periodontitis and the neutrophil collagenase in adult periodontilis gingival crevicular fluid. Furthermore, specific tetracycline inhibition can be used as a simple and practical “probe” to identify the cellular source of interstitial collagenases from in vivo samples.

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Tài liệu tham khảo

10.1111/j.1600-0714.1988.tb01313.x

Burns FR, 1989, Inhibition of purified collagenase from alcali‐burned rabbit corneas, Invest Ophthalmol Vis Sci, 30, 1569

10.1111/j.1600-0765.1990.tb00923.x

10.1177/00220345870660080401

Golub LM, Critical Reviews in Oral Biology

10.1111/j.1600-0765.1985.tb00405.x

10.1056/NEJM199005033221805

Hasty KA, 1990, Human neutrophil collagenase. A distinct gene product with homology to other matrix metarroproteinases, J Biol Chem, 265, 11421, 10.1016/S0021-9258(19)38413-3

Lee HM, 1991, Specificity of the anti‐collagenase action of tetracyclines, J Dent Res, 70, 469

10.1056/NEJM199007123230215

10.1111/j.1600-0765.1988.tb01618.x

10.1111/j.1399-302X.1991.tb00447.x

10.1111/j.1600-0714.1985.tb00474.x

10.1056/NEJM198902093200606

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Thông tin xuất bản

Wiley

Tập 7 Số 2

121-123

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