Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro

Wiley - Tập 23 Số 4 - Trang 336-343 - 2008
Anna Haukioja1, Vuokko Loimaranta2, Jorma Tenovuo3,4
1Postgraduate School of Oral Health Sciences, Institute of Dentistry
2Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine
3Department of Cariology, Institute of Dentistry, University of Turku, Turku, Finland
4Department of Oral Diseases, Turku University Central Hospital, Turku, Finland

Tóm tắt

Introduction:  The use of probiotic bacteria is increasing worldwide and at least some of them can transiently colonize the oral cavity. Several studies have shown that probiotic bacteria, which are often thought of in relation only to intestinal health, can also affect the oral ecology, but the mechanisms for this are largely unknown. The aim of this study was to investigate in vitro if the probiotic bacteria used in commercial products affect the protein composition of the salivary pellicle and the adherence of other oral bacteria.

Methods:  Salivary pellicle on hydroxyapatite and the adhesion of two oral streptococci, Streptococcus mutans and Streptococcus gordonii, were used as a model.

Results:  Probiotic bacteria that bound to saliva‐coated hydroxyapatite reduced the adhesion of S. mutans but the inhibitory effect on the adherence of S. gordonii was weaker. Salivary pellicle protein composition was modified by all the strains tested. The modifications in the pellicle affected the adherence of S. mutans but not of S. gordonii. Two of the proteins missing from the pellicles made of saliva‐treated with the probiotic bacteria were identified as salivary agglutinin gp340 and salivary peroxidase. All bacterial strains bound salivary agglutinin gp340. The ability of the probiotic bacteria to degrade peroxidase was demonstrated with purified bovine lactoperoxidase and two of the probiotic strains.

Conclusion:  This in vitro study showed that probiotic strains used in commercial products may affect the oral ecology by specifically preventing the adherence of other bacteria and by modifying the protein composition of the salivary pellicle.

Từ khóa


Tài liệu tham khảo

10.1016/S0889-5406(03)00346-9

10.1016/S0003-9969(02)00112-7

Alaluusua S, 1983, Streptococcus mutans establishment and dental caries experience in children from 2 to 4 years old, Scand J Dent Res, 91, 453

10.1128/JCM.40.3.1001-1009.2002

10.1016/0167-4838(92)90346-F

10.1159/000260859

10.1111/j.1365-2672.2006.02837.x

10.1080/00016350600801709

10.1080/00016350510020070

10.1177/00220345950740040301

Clark WB, 1978, Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces, Infect Immun, 19, 846, 10.1128/iai.19.3.846-853.1978

10.1111/j.1432-1033.1983.tb07456.x

10.1128/JB.154.1.1-9.1983

Gibbons RJ, 1985, Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles, Infect Immun, 50, 592, 10.1128/iai.50.2.592-594.1985

10.1177/154405910708600204

10.1136/bmj.322.7298.1327

10.1111/j.1399-302X.2006.00299.x

10.1128/IAI.57.12.3702-3707.1989

10.1099/jmm.0.05009-0

10.1128/IAI.73.4.2245-2252.2005

10.1021/bi00401a035

10.1159/000088183

10.1111/j.1600-0722.1995.tb00169.x

10.1159/000076559

10.1159/000047484

10.1093/glycob/10.11.1193

10.1016/j.ijfoodmicro.2004.03.006

10.1016/S1043-4526(03)47004-6

10.1074/jbc.M006928200

10.1128/JB.187.17.6128-6136.2005

Pruitt KM, 1979, Lactoperoxidase binding to streptococci, Infect Immun, 25, 304, 10.1128/iai.25.1.304-309.1979

10.1016/S1286-4579(02)01544-7

10.1159/000262015

10.1016/S1286-4579(00)01316-2

Rundegren J, 1986, Calcium‐dependent salivary agglutinin with reactivity to various oral bacterial species, Infect Immun, 53, 173, 10.1128/iai.53.1.173-178.1986

10.1016/j.copbio.2005.02.003

10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I

10.1111/j.1600-0722.2007.00458.x

10.1016/S0091-6749(96)70245-2

10.1080/000163598428400

10.1159/000261451

10.1046/j.0902-0055.2001.00084.x

10.1111/j.1399-302X.2006.00258.x

Thông tin
Thông tin xuất bản

Wiley

Tập 23 Số 4

336-343

Thông tin tác giả