Differential gene expression of human β‐defensins (hBD‐1, ‐2, ‐3) in inflammatory gingival diseases

Wiley - Tập 20 Số 3 - Trang 186-190 - 2005
Henrik Dommisch1, Yahya Açil2, Anton Dunsche3, Jochen Winter1, Søren Jepsen1
1Department of Periodontology, Operative and Preventive Dentistry, University of Bonn
2Department of Oral and Maxillofacial Surgery, University of Schleswig‐Holstein, Kiel
3Clinic for Oral‐Maxillofacial Surgery, Karlsruhe, Germany

Tóm tắt

Antimicrobial peptides, like human β‐defensins, play an important role in the epithelial innate defense response. The aim of the present study was to investigate the quantitative expression of human β‐defensin‐1, ‐2, and ‐3 in inflammatory gingival diseases. Gingival biopsies were obtained from patients with healthy gingiva (n = 10), patients with gingivitis (n = 10), and patients with periodontitis (n = 10). The clinical diagnosis was verified by histology. Gingival tissues were used for RNA extraction followed by reverse transcription. Gene expression was quantified by real‐time polymerase chain reaction (normalization with GAP‐DH). Comparing the tissues with different clinical stages of health and disease, no significant differences in mRNA expression were found for any of the β‐defensins studied. Similar levels of expression were found in healthy gingiva, whereas in gingivitis samples there was a significantly higher expression of hBD‐2 compared to hBD‐1 (P = 0.004) and hBD‐3 (P = 0.016). Likewise, in periodontitis samples, hBD‐2 expression was significantly higher than hBD‐1 (P = 0.016); however, hBD‐2 expression was comparable to hBD‐3. In conclusion, the results of the present study showed a differential expression of human β‐defensins (hBD‐1, ‐2, ‐3) in tissues with inflammatory gingival disease.

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Tài liệu tham khảo

10.1038/81385

10.1016/0014-5793(95)00687-5

10.1034/j.1600-0757.2002.03007.x

10.1034/j.1600-0714.2001.300601.x

10.1128/IAI.68.1.113-119.2000

10.1034/j.1600-0722.2002.11186.x

10.1034/j.1600-0714.2001.300305.x

Ganz T, 1997, Antimicrobial peptides of phagocytes and epithelia, Semin Hematol, 34, 343

10.1016/S0092-8674(00)81895-4

10.1038/43088

10.1074/jbc.M008557200

10.1165/ajrcmb.22.6.4023

10.1038/84627

10.4049/jimmunol.168.1.316

10.1128/IAI.68.5.2907-2915.2000

Krisanaprakornkit S, 1998, Expression of the peptide antibiotic human beta‐defensin 1 in cultured gingival epithelial cells and gingival tissues, Infect Immun, 66, 4222, 10.1128/IAI.66.9.4222-4228.1998

10.4049/jimmunol.170.1.575

ncbi.nlm.nih.gov/mapview.year.

10.1056/NEJMoa021481

10.1177/00220345000790090601

10.1016/S0006-2952(98)00226-3

10.1073/pnas.042692699

10.1016/S0014-5793(01)03088-5

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Wiley

Tập 20 Số 3

186-190

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