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An overview on current molecular tools for heterologous gene expression in Trichoderma
Springer Science and Business Media LLC - Tập 8 - Trang 1-17 - 2021
Fungi of the genus Trichoderma are routinely used as biocontrol agents and for the production of industrial enzymes. Trichoderma spp. are interesting hosts for heterologous gene expression because their saprotrophic and mycoparasitic lifestyles enable them to thrive on a large number of nutrient sources and some members of this genus are generally recognized as safe (GRAS status). In this review, ......
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
Springer Science and Business Media LLC - Tập 1 - Trang 1-8 - 2014
Pleurotus sapidus secretes a huge enzymatic repertoire including hydrolytic and oxidative enzymes and is an example for higher basidiomycetes being interesting for biotechnology. The complex growth media used for submerged cultivation limit basic physiological analyses of this group of organisms. Using undefined growth media, only little insights into the operation of central carbon metabolism an......
Simple sequence repeat markers that identify Claviceps species and strains
Springer Science and Business Media LLC - Tập 3 - Trang 1-13 - 2016
Claviceps purpurea is a pathogen that infects most members of Pooideae, a subfamily of Poaceae, and causes ergot, a floral disease in which the ovary is replaced with a sclerotium. When the ergot body is accidently consumed by either man or animal in high enough quantities, there is extreme pain, limb loss and sometimes death. This study was initiated to develop simple ......
The fungal composition of natural biofinishes on oil-treated wood
Springer Science and Business Media LLC - Tập 4 - Trang 1-13 - 2017
Biofinished wood is considered to be a decorative and protective material for outdoor constructions, showing advantages compared to traditional treated wood in terms of sustainability and self-repair. Natural dark wood staining fungi are essential to biofinish formation on wood. Although all sorts of outdoor situated timber are subjected to fungal staining, the homogenous dark staining called biof......
Branching and converging pathways in fungal natural product biosynthesis
Springer Science and Business Media LLC - Tập 9 - Trang 1-24 - 2022
In nature, organic molecules with great structural diversity and complexity are synthesized by utilizing a relatively small number of starting materials. A synthetic strategy adopted by nature is pathway branching, in which a common biosynthetic intermediate is transformed into different end products. A natural product can also be synthesized by the fusion of two or more precursors generated from ......
Exploring fungal RiPPs from the perspective of chemical ecology
Springer Science and Business Media LLC - Tập 9 - Trang 1-12 - 2022
Since the initial detection, in 2007, of fungal ribosomally synthesised and post-translationally modified peptides (RiPPs), this group of natural products has undergone rapid expansion, with four separate classes now recognised: amatoxins/phallotoxins, borosins, dikaritins, and epichloëcyclins. Largely due to their historically anthropocentric employment in medicine and agriculture, novel fungal p......
Deletion of the small GTPase rac1 in Trichoderma reesei provokes hyperbranching and impacts growth and cellulase productionAbstract Background Trichoderma reesei is widely known for its enormous protein secretion capacity and as an industrially relevant producer of cellulases and hemicellulases. Over the last decades, rational strain engineering was applied to further enhance homologous and heterologous enzyme yields. The intro...
Springer Science and Business Media LLC - - 2019
Antimicrobial activities of metabolites isolated from endophytic Aspergillus flavus of Sarcophyton ehrenbergi supported by in-silico study and NMR spectroscopy
Springer Science and Business Media LLC - Tập 10 - Trang 1-13 - 2023
Endophytic Aspergillus species produce countless valuable bioactive secondary metabolites. In the current study, Aspergillus flavus an endophyte from the soft coral Sarcophyton ehrenbergi was chemically explored and the extracted phytoconstituents were subsequently evaluated for antimicrobial activity. This is accomplished by employing nuclear magnetic resonance (NMR) spectroscopy and computationa......
Update on Nox function, site of action and regulation in Botrytis cinerea
Springer Science and Business Media LLC - Tập 3 - Trang 1-15 - 2016
The production of reactive oxygen species (ROS) and a balanced redox homeostasis are essential parameters, which control the infection process of the plant pathogen Botrytis cinerea. The necrotrophic fungus is able to cope with the plants’ oxidative burst and even produces its own ROS to overcome the plants’ defense barrier. Major enzyme complexes, which are responsible for the production of super......
Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans
Springer Science and Business Media LLC - Tập 9 - Trang 1-17 - 2022
Extracellular vesicles (EVs) are increasingly recognized as an important mechanism for cell–cell interactions. Their role in fungi is still poorly understood and they have been isolated from only a handful of species. Here, we isolated and characterized EVs from Aureobasidium pullulans, a biotechnologically important black yeast-like fungus that is increasingly used for biocontrol of phytopathogen......
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