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One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli
Springer Science and Business Media LLC - Tập 13 - Trang 1-8 - 2013
Human α-synuclein is a small-sized, natively unfolded protein that in fibrillar form is the primary component of Lewy bodies, the pathological hallmark of Parkinson’s disease. Experimental evidence suggests that α-synuclein aggregation is the key event that triggers neurotoxicity although additional findings have proposed a protective role of α-synuclein against oxidative stress. One way to addres......
The effects of TORC signal interference on lipogenesis in the oleaginous yeast Trichosporon oleaginosus
Springer Science and Business Media LLC - Tập 17 - Trang 1-9 - 2017
Oleaginous organisms are a promising, renewable source of single cell oil. Lipid accumulation is mainly induced by limitation of nutrients such as nitrogen, phosphorus or sulfur. The oleaginous yeast Trichosporon oleaginosus accumulates up to 70% w/w lipid under nitrogen stress, while cultivation in non-limiting media only yields 9% w/w lipid. Uncoupling growth from lipid accumulation is key for t......
A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay
Springer Science and Business Media LLC - Tập 22 - Trang 1-8 - 2022
Lymphocytic leukemia (LL) is a primary malignant tumor of hematopoietic tissue, which seriously affects the health of children and the elderly. The study aims to establish a new detection method for screening acute/chronic LL using time-resolved fluorescence immunoassay (TRFIA) via quantitative detection of S100 calcium binding protein A8 (S100A8) and leucine-rich alpha-2-glycoprotein 1 (LRG1) in ......
Patch cloning method for multiple site-directed and saturation mutagenesis
Springer Science and Business Media LLC - Tập 13 - Trang 1-8 - 2013
Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient tools for cloning and have been applied to site-directed mutagenesis recently. This study describes an optimized......
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
Springer Science and Business Media LLC - Tập 9 - Trang 1-16 - 2009
Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigate the capacity......
Establishment of automated culture system for murine induced pluripotent stem cells
Springer Science and Business Media LLC - Tập 12 - Trang 1-8 - 2012
Induced pluripotent stem (iPS) cells can differentiate into any cell type, which makes them an attractive resource in fields such as regenerative medicine, drug screening, or in vitro toxicology. The most important prerequisite for these industrial applications is stable supply and uniform quality of iPS cells. Variation in quality largely results from differences in handling skills between operat......
Flavonoid engineering of flax potentiate its biotechnological application
Springer Science and Business Media LLC - Tập 11 - Trang 1-19 - 2011
Flavonoids are a group of secondary plant metabolites important for plant growth and development. They show also a protective effect against colon and breast cancer, diabetes, hypercholesterolemic atherosclerosis, lupus nephritis, and immune and inflammatory reactions. Thus, overproduction of these compounds in flax by genetic engineering method might potentiate biotechnological application of the......
A modified fluorimetric host cell reactivation assay to determine the repair capacity of primary keratinocytes, melanocytes and fibroblasts
Springer Science and Business Media LLC - Tập 10 - Trang 1-11 - 2010
The Host Cell Reactivation Assay (HCRA) is widely used to identify circumstances and substances affecting the repair capacity of cells, however, it is restricted by the transfection procedure used and the sensitivity of the detection method. Primary skin cells are particularly difficult to transfect, and therefore sensitive methods are needed to detect any variations due to the cell-type or inter-......
Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
Springer Science and Business Media LLC - Tập 8 - Trang 1-15 - 2008
Efforts to improve the efficiency of non-viral gene delivery require a better understanding of delivery kinetics of DNA molecules into clinically relevant cells. Towards this goal, three DNA molecules were employed to investigate the effects of DNA properties on cellular delivery: a circular plasmid DNA (c-DNA), a linearized plasmid DNA (l-DNA) formulated by single-site digestion of c-DNA, and sma......
Regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9Aa2 gene conferring insect resistance
Springer Science and Business Media LLC - Tập 11 - Trang 1-10 - 2011
The recovery of high performing transgenic lines in clonal crops is limited by the occurrence of somaclonal variation during the tissue culture phase of transformation. This is usually circumvented by developing large populations of transgenic lines, each derived from the first shoot to regenerate from each transformation event. This study investigates a new strategy of assessing multiple shoots i......
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