Anther culture of Lupinus angustifolius: callus formation and the development of multicellular and embryo-like structures

Plant Growth Regulation - Tập 66 - Trang 145-153 - 2011
Kamila Kozak1, Renata Galek1, Mohammad Tahir Waheed2, Ewa Sawicka-Sienkiewicz1
1Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
2Department of Applied Plant Sciences and Plant Biotechnology (DAPP), University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria

Tóm tắt

Androgenesis is an important technique to generate double haploid plants. Anther and microspore cultures are the methods to induce haploid embryogenesis. For culture initiation, it is necessary to select anthers with the appropriate developmental stage of microspores. For lupins, limited reports about the establishment of initial cultures for androgenesis are available. In this study, different parameters of anther culture of three genotypes of Lupinus angustifolius were investigated. For all genotypes, a considerable correlation was observed between the buds and the anthers, depending on their location in the inflorescences. Buds from the central segment of inflorescences had yellowish green anthers that contained the maximum number of microspores at uninucleate stage. Cytological investigation shows that the anthers containing these microspores were the most responsive to induction. Two types of developmental pathways were observed for microspores. In case of cold pre-treated and untreated inflorescences, microspores developed into multicellular and embryo-like structures, respectively. Effects of different factors showed significant differences among: genotypes, pre-treatment, growth regulators (GRs) and genotypes × GRs interaction. Among three genotypes, Emir showed the highest number of multicellular and embryo-like structures on MS medium + 2.0 mg/l 2,4 D + 0.5 mg/l Kinetin (Kin). For all genotypes, anthers produced calli on MS medium containing 2.0 mg/l 2,4 D + 0.5 mg/l Kin. These calli continued their growth on regeneration medium (MS + 2.0 mg/l BA + 0.5 mg/l NAA) and produced roots. Taken together, these results provide a good basis for further research towards the development of haploid plants for L. angustifolius.

Tài liệu tham khảo

Ball ST, Zhou H, Konzak CF (1993) Influence of 2, 4-D, IAA and duration of callus induction in anther culture of spring wheat. Plant Sci 90:195–200

Bayliss KL, Wroth JM, Cowling WA (2004) Pro-embryos of Lupinus spp. produced from isolated microspore culture. Aust J Agr Res 55:589–593

Constantin MJ (1981) Pollen embryogenesis to induce, detect, and analyze mutants. Environ Health Persp 37:27–33

Croser J, Lülsdorf M, Davies P, Wilson J, Sidhu P, Grewal R, Allen K, Dament T, Warkentin T, Vandenberg A, Siddique K (2005) Haploid embryogenesis from chickpea & field pea—progress towards a routine protocol. In: Proceedings of the Australian branch of the IAPTC&B, 21–24th September, Perth, Western Australia, pp 71–82

Croser JS, Lülsdorf MM, Davies PA, Clarke HJ, Bayliss KL, Mallikarjuna N, Siddique KHM (2006) Toward doubled haploid production in the Fabaceae: progress, constraints, and opportunities. Crit Rev Plant Sci 25:139–157

Erdtman G (1952) Pollen morphology and plant taxonomy-Angiosperms (an introduction to palynology). Almqvist and Wiksell, Stockholm

Faluyi MA, Zhou XM, Zhang F, Leibovitch S, Migner P, Smith DL (2000) Seed quality of sweet white lupin (Lupinus albus) and management practice in eastern Canada. Eur J Agron 13:7–37

Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301–309

Grewal RK, Lulsdorf M, Croser J, Ochatt S, Vandenberg A, Warkentin TD (2009) Doubled-haploid production in chickpea (Cicer arietinum L.): role of stress treatments. Plant Cell Rep 28:1289–1299

Huyghe C (1997) White lupin (Lupinus albus L.). Field Crop Res 53:147–160

Jiménez VM (2005) Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul 47:91–110

Kaur P, Bhalla JK (1998) Regeneration of haploid plants from microspore culture of pigeonpea (Cajanus cajan L.). Indian J Exp Biol 36:736–738

Lanas I, Gallego P, Martin L, Fernandez J, Alonsom A, Elena-Rosello J, Blazquez A, Villalobos N, Guerra H (2006) In vitro culture of Medicago arborea L. anthers: initial response. Plant Growth Regul 49:49–60

Lauxen MS, Kaltchuk-Santos E, Hu CY, Callegari-Jacques SM, Bodanese-Zanettini MH (2003) Association between floral bud size and developmental stage in soybean microspores. Braz Arch Biol Techn 46:515–520

Moraes AP, Bonadese-Zanettini MH, Callegari-Jacques SM, Kaltchuk-Santos E (2004) Effect of temperature shock on soybean microspore embryogenesis. Braz Arch Biol Techn 47:537–544

Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum 15:473–497

Ochatt S, Pech C, Grewal R, Conreux C, Lulsdorf M, Jacas L (2009) Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae). J Plant Physiol 166:1314–1328

Ormerod AJ, Caligari PDS (1994) Anther and microspore culture of Lupinus albus in liquid culture medium. Plant Cell Tissue Organ Cult 36:227–236

Sator C (1985) Plant regeneration from anthers of lupins. Landbauforsch Volk 35:5–7

Sator C, Mix G, Menge U (1983) Investigation on anther culture of Lupinus polyphyllus. Plant Res Dev 18:37–46

Skrzypek E, Czyczylo-Mysza I, Marcińska I, Wędzony M (2008) Prospects of androgenetic induction in Lupinus spp. Plant Cell Tissue Organ Cult 94:131–137

Sudhersan C, Manuel SJ, Al-Sabah L (2008) Haploid plant production from pollen grains of Sturt’s Desert Pea via Somatic Embryogenesis. American-Eurasian J Sci Res 3:44–47

Zagorska N, Dimitrov B, Gadeva P, Robeva P (1997) Regeneration and characterization of plants obtained from anther cultures in Medicago sativa L. In Vitro Cell Dev Biol Plant 33:107–110

Zhao G, Liu Y, Li J (1998) Germination of embryo in soybean anther culture. Chin Sci Bull 43:1991–1995

Thông tin
Thông tin xuất bản

Plant Growth Regulation

Tập 66

145-153

Thông tin tác giả