Regeneration and characterization of plants obtained from anther cultures in Medicago sativa L
Tóm tắt
The androgenic ability of four Medicago sativa L. genotype (Boynitza 5, Byala, 494, and 3815) was tested. Callus and organogenesis were induced in all lines studied. The percentage of anthers producing calluses and organogenesis showed wide variation (calluses—from 11% up to 77%; organogenesis—4.8% to 15.2%). It has been established that genotype, nutrient medium composition, and stage of pollen development considerably affected both callus production and organogenesis. Androgenesis in M. sativa could be achieved via callus and direct embryogenesis. About 500 morphologically different regenerants were obtained. Wide variability in chromosome number of regenerated plants was observed by cytological studies. Haploid, dihaploid, as well as mixoploid plants were obtained.
Tài liệu tham khảo
Bajaj, Y. P. S.; Gosch, G.; Ottma, M., et al. Production of polyploid and aneuploid plants from anthers and mesophyll protoplasts of Atropa belladonna and Nicotiana tabacum. Indian J. Exp. Biol. 16:947–963; 1978.
Bajaj, Y. P. S.; Ram, H.; Labana, K., et al. Regeneration of genetically variable plants from the anther-derived callus of Arachis hypogaea and Arachis villosa. Plant Sci. Lett. 23:35–39; 1981.
Bajaj, Y. P. S.; Singh, H.; Gosal, S. Haploid embryogenesis in anther cultures of pigeonpea (Cajanus cajan). Theor. Appl. Genet. 58:157–159; 1980.
Bingham, E. T. Isolation of haploids of tetraploid alfalfa. Crop Sci. 11:433–435; 1971.
Bingham, E. T.; McCoy, T. Cultivated alfalfa at the diploid level: origin of reproductive stability, and yield of seed and forage. Crop Sci. 19:97–100; 1979.
Bingham, E. T.; Saunders, Y. W. Chromosome manipulations in alfalfa: scaling the cultivated tetraploid to seven ploidy levels. Crop Sci. 14:474–477; 1974.
Blaydes, D. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant. 19:748–753; 1966.
Bohorova, N.; Atanassov, A.; Georgieva, J. In vitro organogenesis, androgenesis and embryo culture in the genus Helianthus L. Z. Pflanzenzucht. 95:35–44; 1985.
Chen, C.; Kasha, K.; Marsolais, A. Sedimentation patterns and mechanisms of genome in cultured microspores of barley. Can. J. Genet. Cytol. 26:475–483; 1984.
Gamborg, O.; Miller, R.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.
Genovesi, A. Maize (Zea mays Z.): in vitro production of haploids. In: Y. P. S. Bajaj, ed. Biotechnology in forestry. Germany: Springer-Verlag; 1990:176–202.
Gharyal, P.; Maheshwari, S. Androgenic response from cultured anthers of a leguminous tree. Cassia siamea Zam. Protoplasma 118:91–93; 1983.
Gosal, S.; Bajaj, Y. P. S. Establishment of callus tissue cultures and the induction of organogenesis in some grain legumes. Crop Improv. 6:154–160; 1979.
Groos, R. W.; Bingham, E. T. Variation in plants regenerated from tissue culture of tetraploid alfalfa heterozygous for several traits. Crop. Sci. 24:655–658; 1984.
Groos, R. W.; Bingham, E. T. Gametophytic heterosis for in vitro pollen traits in alfalfa. Crop. Sci. 31:1510–1513; 1991.
Groos, R. W.; Kojis, W. P.; Bingham, E. T. Combining ability differences between isogenic diploid and tetraploid alfalfa. Crop. Sci. 28:7–10; 1988.
Hesemann, C. Haploide zellen in kalli aus antheren kulturen von Vicia faba. Pflanzenzucht. 84:18–22; 1980.
Hu, H. Wheat: improvement through anther culture. In: Bajaj, Y. P. S., ed. Biotechnology in forestry. Berlin, Germany: Springer-Verlag; 1985:2–55.
McCoy, T. W.; Walker, K. Alfalfa. In: Ammirato, P. V.; Evans, D. A.; Sharp, W. R., et al., eds. Handbook of plant cell culture. New York: Macmillan Publishing Co.; 1984:171–192.
Mix, G.; Wilson, H.; Foroughi-Wehr, B. The cytological status plants of Hordeum vulgare L. regenerated from microspore callus. Pflanzenzuchtg. 80:89–99; 1978.
Mohan, H. R.; Menta, U.; Ramanuja, I. R., et al. Haploid induction in legumes. In: Fujiwara, A., ed. Plant tissue culture. Tokyo: Maruzen; 1982:541–542.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.
Niizeki, H.; Oono, K. Rice plants obtained by anther culture. Les cultures de tissue de plantes. Coll. Int. CNRS 193:251–257; 1971.
Peters, J.; Crocomo, O.; Sharp, W., et al. Haploid callus cells from anthers of Phaseolus vulgaris. Phytomorphology 27:79–85; 1977.
Ray, I. M.; Bingham, E. T. Breeding diploid alfalfa for regeneration from tissue culture. Crop Sci. 29:1545–1548; 1989.
Sator, C. Regeneration von upinenpflanzen aus antheren. Landbauforsch. Voelkenrode 35:5–7; 1985.
Saunders, Y. W.; Bingham, E. T. Production of alfalfa plants from callus tissue. Crop. Sci. 12:804–808; 1972.
Thipmani, P. Pathway of pollen androgenesis in field bean. Abstract of the International Symposium on the Genetic Manipulation of Crops; 2:1984.
Wenzel, Y.; Uhrig, H. Breeding for nematode and virus resistance in potato via anther culture. Theor. Appl. Genet. 59:333–340; 1981.
Zagorska, N.; Palakarcheva, M.; Shabanov, D., et al. Homozygous tobacco obtained via induced androgenesis. Genet. Breed. 11:177–186; 1978.
Zagorska, N.; Robeva, P.; Dimitrov, B., et al. Induction of regeneration in anther cultures in Medicago sativa L. C. R. Acad. Bulg. Sci. 37:1099–1102; 1984.
Zagorska, N.; Dimitrov, B. Induced androgenesis in alfalfa (Medicago sativa L.). Plant Cell Rep. 14:249–252; 1995.