Prospects of androgenetic induction in Lupinus spp.

Plant Cell, Tissue and Organ Culture - Tập 94 - Trang 131-137 - 2008
Edyta Skrzypek1, Ilona Czyczyło-Mysza1, Izabela Marcińska1, Maria Wędzony1,2
1Polish Academy of Sciences, The F. Górski Institute of Plant Physiology, Krakow, Poland
2Pedagogical University of Cracow, Krakow, Poland

Tóm tắt

Anthers cultures of six Polish cultivars of pasture lupin (Lupinus L.) were examined for their androgenic response. Anthers with microspores at the uninucleate stage were isolated from flower buds and cultured in liquid media. Better viability of androgenetic structures was obtained when donor plants had grown under field as opposed to greenhouse conditions. A density of five anthers per 0.5 ml medium was more conducive to androgenetic induction than 25 anthers per 0.5 ml medium. Addition of 5% maltose to the induction medium and culture at 25°C without pre-treatment of flowers, buds or anthers promoted microspore release and division. The greatest frequency of androgenic callus, ~70% was developed from cvs. Katon, Wat (white lupin), in contrast to cvs. Legat, Juno (yellow lupin), Polonez and Sonet (narrow-leafed lupin) with callus induction ~30–40%. Despite various combinations of media tested, plant regeneration was not obtained from anther derived callus.

Tài liệu tham khảo

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