Germination of embryo in soybean anther culture

A large number of experiments about induction of callus, embryo and germination of regeneration plants were done in soybean anther culture. Screening for genotypes, low-temperature pretreatment for floral buds, constituent of hormone in medium and effects of different temperatures on induction rate of callus and embryo were studied. It was found that the TDZ played an important role in embryo induction and germination. 28 genotypes had reaction competent to the anther culture in all 110 genotypes. Induction rate of callus was 26%–68.1%. Plant differentiation rate was 0.5%–2.0 %.