Wiley
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Các bài báo tiêu biểu
Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergentsAbstract Treatment of isolated human erythrocyte membranes with Triton X‐100 at ionic strength ⋍0.04 preferentially released all the glycerolipid and glycoprotein species. At low ionic strength, certain nonglycosylated polypeptides were also selectively solubilized. The liberated polypeptides were free of lipids, but some behaved as if associated into specific oligo... ...
Tập 1 Số 3 - Trang 233-248 - 1973
Influence of increased membrane cholesterol on membrane fluidity and cell function in human red blood cellsAbstract Cholesterol and phospholipid are the two major lipids of the red cell membrane. Cholesterol is insoluble in water but is solubilized by phospholipids both in membranes and in plasma lipoproteins. Morever, cholesterol exchanges between membranes and lipoproteins. An equilibrium partition is established based on the amount of cholesterol relative to phospholi... ...
Tập 8 Số 4 - Trang 413-430 - 1978
Existence of a common precursor to ACTH and endorphin in the anterior and intermediate lobes of the rat pituaitaryAbstract Extracts of rat anterior and intermediate‐posterior pituitary were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and assayed for immunoactive ACTH and endorphin. In both lobes the major forms of immunoactive ACTH have apparent molecular weights of 31,000 (31K), 20–21K, 14K, and 4.5K, and the major forms of immunoactive endorphin ... ...
Tập 8 Số 3 - Trang 247-262 - 1978
Measurement of the lateral mobility of cell surface components in single living cells by fluorescence recovery after photobleachingAbstract The use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin‐receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin receptor (WGA‐receptor) complexes on the surface of human embryo fibroblasts ... ...
Tập 5 Số 4 - Trang 565-576 - 1976
Growth factors and gangliosides: A possible new perspective in neuronal growth controlAbstract For many permanent cell lines the transition from a growing (P) to a resting (R) state is reversibly controlled by growth factors present in serum. This P‐to‐R transition was studied in a neuronal cell line (B 104) with respect to the action of serum, dibutyryl cyclic AMP (DBcAMP), gangliosides, and a glioma cell‐produced growth factor GGF. In this cell sys... ...
Tập 10 Số 2 - Trang 111-124 - 1979
Reconstituted low density lipoprotein: A vehicle for the delivery of hydrophobic fluorescent probes to cellsAbstract Previous studies have shown that the cholesteryl ester core of plasma low density lipoprotein (LDL) can be extracted with heptane and replaced with a variety of hydrophobic molecules. In the present report we use this reconstitution technique to incorporate two fluorescent probes, 3‐pyrenemethyl‐23, 24‐dinor ‐5‐cholen‐22‐oate‐3β‐yl... ...
Tập 10 Số 4 - Trang 467-478 - 1979
The use of aryl azido ATP analogs as photoaffinity labels for myosin ATPaseAbstract The synthesis of ATP analogs containing a photoactive aryl azido grouping coupled to the 3′ hydroxyl of ATP is described. The potential effectiveness of these analogs in the investigation of nucleotide‐binding regions is outlined and this effectiveness demonstrated by their photodependent inhibition of subfragment 1 ATPase. The use of 14...
Tập 3 Số 5-6 - Trang 448-468 - 1975
The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determinationAbstract In part I of this paper (1) we give evidence that the P23‐capsoid of τ‐particles is transformed in situ into the P23*‐capsid of normal phage. Using the polymorphism of phage T4, we have chosen polyheads as representative of P23 assemblies and giant phages as representative of P23* assemblies in order to study their surface crystals by optical filtration of ... ...
Tập 2 Số 2-4 - Trang 253-275 - 1974
Regulation of dome formation in differentiated epithelial cell culturesAbstract Rat mammary (Rama 25) and dog kidney (MDCK) epithelial cell cultures formed ‘domes’ of cells due to fluid accumulation in focal regions between the culture dish and the cell monolayer. Addition of ouabain caused collapse of domes, suggesting that transport functions were required for maintenance of domes. Dome formation in both epithelial ce... ...
Tập 12 Số 2 - Trang 259-272 - 1979
Co‐translational modification of nascent immunoglobulin heavy and light chainsAbstract We have investigated the in vivo co‐translational covalent modification of nascent immunoglobulin heavy and light chains. Nascent polypeptides were separated from completed polypeptides by ion‐exchange chromatography of solubilized ribosomes on QAE‐Sephadex. First, we have demonstrated that MPC 11 nascent heavy chains are quantitatively glycosylated very so... ...
Tập 11 Số 1 - Trang 9-24 - 1979