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Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents
Tập 1 Số 3 - Trang 233-248 - 1973
AbstractTreatment of isolated human erythrocyte membranes with Triton X‐100 at
ionic strength ⋍0.04 preferentially released all the glycerolipid and
glycoprotein species. At low ionic strength, certain nonglycosylated
polypeptides were also selectively solubilized. The liberated polypeptides were
free of lipids, but some behaved as if associated into specific oligomeric
complexes. Each detergent‐i...
Influence of increased membrane cholesterol on membrane fluidity and cell function in human red blood cells
Tập 8 Số 4 - Trang 413-430 - 1978
AbstractCholesterol and phospholipid are the two major lipids of the red cell
membrane. Cholesterol is insoluble in water but is solubilized by phospholipids
both in membranes and in plasma lipoproteins. Morever, cholesterol exchanges
between membranes and lipoproteins. An equilibrium partition is established
based on the amount of cholesterol relative to phospholipid (C/PL) in these two
compartme...
Existence of a common precursor to ACTH and endorphin in the anterior and intermediate lobes of the rat pituaitary
Tập 8 Số 3 - Trang 247-262 - 1978
AbstractExtracts of rat anterior and intermediate‐posterior pituitary were
fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and
assayed for immunoactive ACTH and endorphin. In both lobes the major forms of
immunoactive ACTH have apparent molecular weights of 31,000 (31K), 20–21K, 14K,
and 4.5K, and the major forms of immunoactive endorphin have apparent molecular
weights o...
Measurement of the lateral mobility of cell surface components in single living cells by fluorescence recovery after photobleaching
Tập 5 Số 4 - Trang 565-576 - 1976
AbstractThe use of fluorescence recovery after photobleaching (FRAP) techniques
to monitor the lateral mobility of plant lectin‐receptor complexes on the
surface of single, living mammalian cells is described in detail. FRAP
measurements indicate that over 75% of the wheat germ agglutinin receptor
(WGA‐receptor) complexes on the surface of human embryo fibroblasts are mobile.
These WGA‐receptor co...
Growth factors and gangliosides: A possible new perspective in neuronal growth control
Tập 10 Số 2 - Trang 111-124 - 1979
AbstractFor many permanent cell lines the transition from a growing (P) to a
resting (R) state is reversibly controlled by growth factors present in serum.
This P‐to‐R transition was studied in a neuronal cell line (B 104) with respect
to the action of serum, dibutyryl cyclic AMP (DBcAMP), gangliosides, and a
glioma cell‐produced growth factor GGF. In this cell system gangliosides seem to
act as d...
Reconstituted low density lipoprotein: A vehicle for the delivery of hydrophobic fluorescent probes to cells
Tập 10 Số 4 - Trang 467-478 - 1979
AbstractPrevious studies have shown that the cholesteryl ester core of plasma
low density lipoprotein (LDL) can be extracted with heptane and replaced with a
variety of hydrophobic molecules. In the present report we use this
reconstitution technique to incorporate two fluorescent probes,
3‐pyrenemethyl‐23, 24‐dinor‐5‐cholen‐22‐oate‐3β‐yl oleate (PMCA oleate) and
dioleyl fluorescein, into heptane‐...
The use of aryl azido ATP analogs as photoaffinity labels for myosin ATPase
Tập 3 Số 5-6 - Trang 448-468 - 1975
AbstractThe synthesis of ATP analogs containing a photoactive aryl azido
grouping coupled to the 3′ hydroxyl of ATP is described. The potential
effectiveness of these analogs in the investigation of nucleotide‐binding
regions is outlined and this effectiveness demonstrated by their photodependent
inhibition of subfragment 1 ATPase. The use of 14C‐labeled azido ATP
demonstrates an almost stoichiome...
The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination
Tập 2 Số 2-4 - Trang 253-275 - 1974
AbstractIn part I of this paper (1) we give evidence that the P23‐capsoid of
τ‐particles is transformed in situ into the P23*‐capsid of normal phage. Using
the polymorphism of phage T4, we have chosen polyheads as representative of P23
assemblies and giant phages as representative of P23* assemblies in order to
study their surface crystals by optical filtration of micrographs. We found for
polyhea...
Regulation of dome formation in differentiated epithelial cell cultures
Tập 12 Số 2 - Trang 259-272 - 1979
AbstractRat mammary (Rama 25) and dog kidney (MDCK) epithelial cell cultures
formed ‘domes’ of cells due to fluid accumulation in focal regions between the
culture dish and the cell monolayer. Addition of ouabain caused collapse of
domes, suggesting that transport functions were required for maintenance of
domes.Dome formation in both epithelial cell lines was stimulated by a broad
spectrum of kno...
Co‐translational modification of nascent immunoglobulin heavy and light chains
Tập 11 Số 1 - Trang 9-24 - 1979
AbstractWe have investigated the in vivo co‐translational covalent modification
of nascent immunoglobulin heavy and light chains. Nascent polypeptides were
separated from completed polypeptides by ion‐exchange chromatography of
solubilized ribosomes on QAE‐Sephadex. First, we have demonstrated that MPC 11
nascent heavy chains are quantitatively glycosylated very soon after the
asparaginyl acceptor...