Wiley

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A regulator protein for the length determination of bacteriophage lambda tail
Wiley - Tập 2 Số 2-4 - Trang 239-252 - 1974
Katsura Izui, Peter Kuhl
AbstractMutants in gene U of phage λ produce polytails. Those polytails have a tail fiber and a basal part like normal tails, but their major tubular part is longer than that of normal tails and shows a wide length distribution.We established the morphogenetic pathway of the λ tail and found that U gene product (pU) acts throughout the assembly of ...... hiện toàn bộ
The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination
Wiley - Tập 2 Số 2-4 - Trang 253-275 - 1974
Ueli Aebi, R.K.L. Bijlenga, Josef M. Bro ek, R. van den Broek, Frederick A. Eiserling, Colleen A. Kellenberger, E. Kellenberger, Vadim V. Mesyanzhinov, L. Müller, Michael K. Showe, Paul Smith, Alasdair C. Steven
AbstractIn part I of this paper (1) we give evidence that the P23‐capsoid of τ‐particles is transformed in situ into the P23*‐capsid of normal phage. Using the polymorphism of phage T4, we have chosen polyheads as representative of P23 assemblies and giant phages as representative of P23* assemblies in order to study their surface crystals by optical filtration of ...... hiện toàn bộ
The regional association of actin and myosin with sites of particle phagocytosis
Wiley - Tập 12 Số 3 - Trang 369-384 - 1979
Richard G. Painter, Ann Tozier McIntosh
AbstractContractile proteins are thought to play a causative role in motile processes such as phagocytosis. In order to investigate their role in phagocytosis further, simultaneous immunofluorescence localization of F‐actin and myosin was carried out in resident mouse peritoneal macrophages after phagocytosis of opsonized zymosan particles. Both actin and myosin ap...... hiện toàn bộ
The use of aryl azido ATP analogs as photoaffinity labels for myosin ATPase
Wiley - Tập 3 Số 5-6 - Trang 448-468 - 1975
Stella J. Jeng, Richard J. Guillory
AbstractThe synthesis of ATP analogs containing a photoactive aryl azido grouping coupled to the 3′ hydroxyl of ATP is described. The potential effectiveness of these analogs in the investigation of nucleotide‐binding regions is outlined and this effectiveness demonstrated by their photodependent inhibition of subfragment 1 ATPase. The use of 14... hiện toàn bộ
Molecular interactions in the bacterial phosphoenolpyruvate‐phosphotransferase system (PTS)
Wiley - Tập 2 Số 5-6 - Trang 695-714 - 1974
Werner Kunding
Influence of increased membrane cholesterol on membrane fluidity and cell function in human red blood cells
Wiley - Tập 8 Số 4 - Trang 413-430 - 1978
Richard A. Cooper
AbstractCholesterol and phospholipid are the two major lipids of the red cell membrane. Cholesterol is insoluble in water but is solubilized by phospholipids both in membranes and in plasma lipoproteins. Morever, cholesterol exchanges between membranes and lipoproteins. An equilibrium partition is established based on the amount of cholesterol relative to phospholi...... hiện toàn bộ
Subcellular fractionation of brown adipose tissue
Wiley - Tập 11 Số 4 - Trang 445-449 - 1979
Jean-Paul Giacobino
AbstractThe present study proposes a technique, using Metrizamide, which permits the preparation of brown adipose tissue plasma membranes from the crude mitochondria as well as from the crude microsome fraction. These plasma membranes have high relative specific activities of their marker enzyme, 5′‐nucleotidase (15 ± 3 and 14 ± 2 respectively) and, particularly th...... hiện toàn bộ
Measurement of the lateral mobility of cell surface components in single living cells by fluorescence recovery after photobleaching
Wiley - Tập 5 Số 4 - Trang 565-576 - 1976
Kenneth A. Jacobson, Zenon Derzko, E. S. Wu, Y. Hou, George Poste
AbstractThe use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin‐receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin receptor (WGA‐receptor) complexes on the surface of human embryo fibroblasts ...... hiện toàn bộ
Carbon‐13 as a tool for the study of carbohydrate structures, conformations and interactions
Wiley - Tập 6 Số 4 - Trang 535-550 - 1977
Hernan A. Nunez, Thomas E. Walker, Rolly G. Fuentes, John O’Connor, A. Serianni, Robert Barker
AbstractThe application of 13C‐NMR spectroscopy to problems involving the structures and interactions of carbohydrates is described. Both 13C‐enriched and natural abundance compounds were used and some advantages of the use of the stable isotope are described. Carbon‐carbon and carbon‐proton coupling constants obtained from...... hiện toàn bộ
Studies related to the head‐maturation pathway of bacteriophages T4 and T2: I. Morphology and kinetics of intracellular particles produced by mutants in the maturation genes
Wiley - Tập 7 Số 2 - Trang 135-161 - 1977
Heidi Wunderli, Josef M. Bro ek, E. Kellenberger
AbstractMutants in the genes governing the maturation of the head of bacteriophage T4 and in gene 24 were studied by electron microscopy of thin sections. We define morphologically: black particles, comprising mature, stable heads and immature, fragile heads, which break down upon lysis; grizzled particles, which apparently are partially filled or partially emptied...... hiện toàn bộ
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