Measurement of the lateral mobility of cell surface components in single living cells by fluorescence recovery after photobleaching

Wiley - Tập 5 Số 4 - Trang 565-576 - 1976
Kenneth A. Jacobson1, Zenon Derzko2, E. S. Wu2, Y. Hou1, George Poste1
1Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, New York 14263
2Roswell Park Cancer Institute

Tóm tắt

AbstractThe use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin‐receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin receptor (WGA‐receptor) complexes on the surface of human embryo fibroblasts are mobile. These WGA‐receptor complexes diffuse laterally (as opposed to flow) on the cell surface with a diffusion coefficient in the range of 2 × 10−11 to 2 × 10−10 cm2/sec. Both the percentage of mobile WGA‐receptor complexes and the mean diffusion coefficient of these complexes are higher than that obtained from earlier FRAP measurements of the mobility of concanavalin A‐receptor (Con A‐receptor) complexes in a variety of cell types. The possible reasons for the differing mobilities of WGA and Con A receptors are discussed.

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Tài liệu tham khảo

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Wiley

Tập 5 Số 4

565-576

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