Wiley
SCOPUS (1980-2002,2004)
0196-4763
1097-0320
Cơ quản chủ quản: N/A
Các bài báo tiêu biểu
Đặc điểm của tế bào theo cơ chế apoptosis được đo bằng lưu lượng tế bào học Dịch bởi AI
Tập 13 Số 8 - Trang 795-808 - 1992
Tóm tắtBài tổng quan này mô tả nhiều phương pháp để nhận diện và phân biệt giữa
hai cơ chế chết tế bào khác nhau, apoptosis và hoại tử. Đa phần các phương pháp
này đã được áp dụng trong các nghiên cứu về apoptosis trong dòng tế bào bạch cầu
HL-60 của người bị kích hoạt bởi các chất ức chế DNA topoizomeras I hoặc II, và
trong các tế bào tuyến ức của chuột bởi cả chất ức chế topoizomeras hoặc
predni...
#Apoptosis #necrosis #lưu lượng tế bào học #HL-60 #tế bào tuyến ức #DNA topoizomeras #dấu hiệu sinh hóa #phân biệt tế bào chết #phương pháp phân định tế bào.
A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysis
Tập 3 Số 5 - Trang 323-327 - 1983
AbstractA new modification of our detergent technique for the preparation of
nuclei for flow cytometric DNA analysis is described. The attainment of low
coefficients of variation of the peaks and of quantitative staining of nuclei
from different tissues was a problem with the original method. This was solved
in the new modification by trypsinization of the unfixed nuclei. The nuclei were
stabilize...
Dead cell discrimination with 7‐amino‐actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry
Tập 13 Số 2 - Trang 204-208 - 1992
AbstractIdentification of nonviable cells in immunofluorescently stained cell
populations is essential for obtaining accurate data. Fluorescent non‐vital DNA
dyes, particularly propidium iodide (PI), have been used routinely in flow
cytometry for discrimination of dead cells from viable cells on the basis of
fluorescence. We describe here the use of an alternative DNA dye,
7‐amino‐actinomycin D (7...
Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probes
Tập 47 Số 4 - Trang 236-242 - 2002
AbstractBackgroundBioreduction of water‐soluble tetrazolium salts (e.g., MTS,
XTT, and MTT) to their respective formazans is generally regarded as an
indicator of cell “redox activity.” The reaction is attributed mainly to
mitochondrial enzymes and electron carriers. However, MTT reduction may also be
catalyzed by a number of other nonmitochondrial enzymes. The goal of this work
was to establish t...
Modified histogram subtraction technique for analysis of flow cytometry data
Tập 9 Số 6 - Trang 619-626 - 1988
AbstractAnalysis of flow cytometry histogram data by the subjective selection of
an integration window can be a tedious and time‐consuming task and is often
inaccurate. A new method for automated calculation of the percent positive from
immunofluorescence histograms is presented. This new method is a modification of
the currently used method of channel‐by‐channel histogram subtraction. Its
accurac...
Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry
Tập 15 Số 1 - Trang 12-20 - 1994
AbstractA rapid, gentle, and sensitive method for quantification of cells
undergoing apoptosis is presented. The method allows the simultaneous
determination of dual‐color cell surface immunofluorescence. Cells are stained
for 7 min with the vital dye Hoechst 33342 (HO342) for identification of live
and apoptotic cells. 7‐amino‐actinomycin D (7‐AAD) is added to distinguish cells
that have lost mem...
Flow cytometric detection of activated mouse integrin αIIbβ3 with a novel monoclonal antibody
Tập 48 Số 2 - Trang 80-86 - 2002
AbstractBackgroundIntegrin αIIbβ3 mediates platelet adhesion and aggregation and
plays a crucial role in thrombosis and hemostasis. αIIbβ3 is expressed in a low
affinity state on resting platelets. Upon platelet activation, αIIbβ3 shifts to
a high affinity conformation that efficiently binds its ligands. On human
platelets, the high affinity conformation of αIIbβ3 is detected by the
monoclonal ant...
Fluorescence energy transfer measurements on cell surfaces: A critical comparison of steady‐state fluorimetric and flow cytometric methods
Tập 5 Số 2 - Trang 210-216 - 1984
AbstractThe energy transfer efficiency E was measured between
fluorescein‐conjugated concanavalin A (Con A) and rhodamine‐conjugated Con A
bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line.
Results from a flow cytometric energy transfer method (FCET) and two different
steady‐state fluorimeter methods were compared. The data were found to be in
close agreement after care...
Optimal detection of apoptosis by flow cytometry depends on cell morphology
Tập 14 Số 8 - Trang 891-897 - 1993
AbstractFlow cytometry has recently become a choice technique for the
quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows
identification of apoptotic cells as a “subdiploid” peak. Progression through
apoptosis leads to chromatin condensation, nuclear fragmentation and eventually
to cell disruption. Thus, a major problem for the flow cytometric analysis of
apoptotic popul...
Nucleic acid dyes for detection of apoptosis in live cells
Tập 21 Số 3 - Trang 265-274 - 1995
AbstractApoptotic thymocytes were found to be much dimmer than normal thymocytes
when stained with several nucleic acid dyes. These dyes provide a quick and
simple assay for apoptosis which works for live cells and does not require a UV
laser. The collection of dyes giving this staining pattern includes reagents
suitable for use in either the FL1, FL2, or FL3 channel of a standard FACScan.
Cells i...