Wiley
0196-4763
1097-0320
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Các bài báo tiêu biểu
Đặc điểm của tế bào theo cơ chế apoptosis được đo bằng lưu lượng tế bào học Dịch bởi AI Tóm tắt Bài tổng quan này mô tả nhiều phương pháp để nhận diện và phân biệt giữa hai cơ chế chết tế bào khác nhau, apoptosis và hoại tử. Đa phần các phương pháp này đã được áp dụng trong các nghiên cứu về apoptosis trong dòng tế bào bạch cầu HL-60 của người bị kích hoạt bởi các chất ức chế DNA topoizomeras I hoặc II, và trong các tế bào tuyến ức của chuột bởi cả chấ... ...
Tập 13 Số 8 - Trang 795-808 - 1992
#Apoptosis #necrosis #lưu lượng tế bào học #HL-60 #tế bào tuyến ức #DNA topoizomeras #dấu hiệu sinh hóa #phân biệt tế bào chết #phương pháp phân định tế bào.
A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysisAbstract A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solved in the new modification by trypsinization of the unfixed ... ...
Tập 3 Số 5 - Trang 323-327 - 1983
Dead cell discrimination with 7‐amino‐actinomcin D in combination with dual color immunofluorescence in single laser flow cytometryAbstract Identification of nonviable cells in immunofluorescently stained cell populations is essential for obtaining accurate data. Fluorescent non‐vital DNA dyes, particularly propidium iodide (PI), have been used routinely in flow cytometry for discrimination of dead cells from viable cells on the basis of fluorescence. We describe here the use of an alternative ... ...
Tập 13 Số 2 - Trang 204-208 - 1992
Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probesAbstract Background Bioreduction of water‐soluble tetrazolium salts (e.g., MTS, XTT, and MTT) to their respective formazans is generally regarded as an indicator of cell “redox activity.” The reaction is attributed mainly to mitochondrial enzymes and electron carriers. However, MTT reduction may also be catalyzed by a number of othe...
Tập 47 Số 4 - Trang 236-242 - 2002
Modified histogram subtraction technique for analysis of flow cytometry dataAbstract Analysis of flow cytometry histogram data by the subjective selection of an integration window can be a tedious and time‐consuming task and is often inaccurate. A new method for automated calculation of the percent positive from immunofluorescence histograms is presented. This new method is a modification of the currently used method of channel‐by‐channel h... ...
Tập 9 Số 6 - Trang 619-626 - 1988
Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometryAbstract A rapid, gentle, and sensitive method for quantification of cells undergoing apoptosis is presented. The method allows the simultaneous determination of dual‐color cell surface immunofluorescence. Cells are stained for 7 min with the vital dye Hoechst 33342 (HO342) for identification of live and apoptotic cells. 7‐amino‐actinomycin D (7‐AAD) is added to dis... ...
Tập 15 Số 1 - Trang 12-20 - 1994
Flow cytometric detection of activated mouse integrin αIIbβ3 with a novel monoclonal antibodyAbstract Background Integrin αIIbβ3 mediates platelet adhesion and aggregation and plays a crucial role in thrombosis and hemostasis. αIIbβ3 is expressed in a low affinity state on resting platelets. Upon platelet activation, αIIbβ3 shifts to a high affinity conformation that efficiently binds its ligands. On human platelets, the hi...
Tập 48 Số 2 - Trang 80-86 - 2002
Fluorescence energy transfer measurements on cell surfaces: A critical comparison of steady‐state fluorimetric and flow cytometric methodsAbstract The energy transfer efficiency E was measured between fluorescein‐conjugated concanavalin A (Con A) and rhodamine‐conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady‐state fluorimeter methods were compared. The data were found to... ...
Tập 5 Số 2 - Trang 210-216 - 1984
Optimal detection of apoptosis by flow cytometry depends on cell morphologyAbstract Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a “subdiploid” peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytom... ...
Tập 14 Số 8 - Trang 891-897 - 1993
Nucleic acid dyes for detection of apoptosis in live cellsAbstract Apoptotic thymocytes were found to be much dimmer than normal thymocytes when stained with several nucleic acid dyes. These dyes provide a quick and simple assay for apoptosis which works for live cells and does not require a UV laser. The collection of dyes giving this staining pattern includes reagents suitable for use in either the FL1, FL2, or FL3 chann... ...
Tập 21 Số 3 - Trang 265-274 - 1995