Wiley
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Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probes
Wiley - Tập 47 Số 4 - Trang 236-242 - 2002
AbstractBackgroundBioreduction of water‐soluble tetrazolium salts (e.g., MTS,
XTT, and MTT) to their respective formazans is generally regarded as an
indicator of cell “redox activity.” The reaction is attributed mainly to
mitochondrial enzymes and electron carriers. However, MTT reduction may also be
catalyzed by a number of other nonmitochondrial enzymes. The goal of this work
was to establish t...
Optimal detection of apoptosis by flow cytometry depends on cell morphology
Wiley - Tập 14 Số 8 - Trang 891-897 - 1993
AbstractFlow cytometry has recently become a choice technique for the
quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows
identification of apoptotic cells as a “subdiploid” peak. Progression through
apoptosis leads to chromatin condensation, nuclear fragmentation and eventually
to cell disruption. Thus, a major problem for the flow cytometric analysis of
apoptotic popul...
Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry
Wiley - Tập 15 Số 1 - Trang 12-20 - 1994
AbstractA rapid, gentle, and sensitive method for quantification of cells
undergoing apoptosis is presented. The method allows the simultaneous
determination of dual‐color cell surface immunofluorescence. Cells are stained
for 7 min with the vital dye Hoechst 33342 (HO342) for identification of live
and apoptotic cells. 7‐amino‐actinomycin D (7‐AAD) is added to distinguish cells
that have lost mem...
Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity
Wiley - Tập 47 Số 3 - Trang 158-162 - 2002
AbstractBackgroundFlow‐cytometric assays are convenient alternatives to classic
radioactive natural killer (NK) tests. MitoTracker Green FM, a green fluorescent
intracellular probe serving originally for staining mitochondria, seemed
especially suitable for labeling NK target cells.Materials and MethodsNK target
cells were labeled with MitoTracker Green FM. After incubation with effector
spleen ce...
Identification and quantitation of apoptotic cells following anti‐CD3 activation of murine G0 T cells
Wiley - Tập 14 Số 8 - Trang 883-890 - 1993
AbstractMultiparameter flow cytometry and cell sorting were used to examine the
process of apoptosis after activation of murine resting T cells with immobilized
anti‐CD3. Activated T cells treated with Hoechst 33342 (HO‐33342) and analyzed
by flow cytometry showed two major cell populations of high and low
fluorescence. These populations were sorted and the DNA extracted and subjected
to electroph...
Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients
Wiley - Tập 50 Số 6 - Trang 298-304 - 2002
AbstractTen years ago, we made an incidental flow cytometric observation while
immunophenotyping biopsy and marrow samples from children suspected to have
leukemia/non‐Hodgkin's lymphoma, but were subsequently diagnosed with
neuroblastoma. The samples contained neoplastic CD45− cells that had an
extremely bright CD56+ (beyond the fourth decade on a four‐decade scale)
population distinguishable fro...
Optical method for measurement of number and shape of attached cells in real time
Wiley - Tập 19 Số 2 - Trang 97-102 - 1995
AbstractA novel, on‐line method of accurately parametrizing the shape of
spreading cells is described. The substrate on which the cells are deposited
serves as an optical waveguide. The method is based on the interaction between
the evanescent part of the guided waves and the cells. No special labelling of
the cells is required. A mathematical framework for interpreting the results is
derived, and...
Rapid quantification of lymphocyte subsets in heterogeneous cell populations by flow cytometry
Wiley - Tập 16 Số 2 - Trang 152-159 - 1994
AbstractDetermination of the number of viable cells or quantification of
lymphocyte subsets in heterogeneous cell populations is critically important for
cytotoxicity assays, apoptosis assays, or the analysis of differential
activation of T‐cell subsets by distinct stimuli. In this report, we describe a
rapid flow cytometry method termed Standard Cell Dilution Analysis (SCDA)
specifically to quant...
The multivariate prognostic index and nuclear DNA content are independent prognostic factors in primary breast cancer patients
Wiley - Tập 10 Số 1 - Trang 56-61 - 1989
AbstractThe predictive value of a previously described Multivariate Prognostic
Index (which incorporates weighted values of the mitotic activity index, tumor
size, and the axillary lymph node status), and the nuclear DNA content (DNA) was
evaluated in 156 patients with primary invasive ductal breast cancer, diagnosed
between 1980 and 1983. The results were analysed with respect to the occurrence
o...
Nucleic acid dyes for detection of apoptosis in live cells
Wiley - Tập 21 Số 3 - Trang 265-274 - 1995
AbstractApoptotic thymocytes were found to be much dimmer than normal thymocytes
when stained with several nucleic acid dyes. These dyes provide a quick and
simple assay for apoptosis which works for live cells and does not require a UV
laser. The collection of dyes giving this staining pattern includes reagents
suitable for use in either the FL1, FL2, or FL3 channel of a standard FACScan.
Cells i...
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