Wiley

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Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probes
Wiley - Tập 47 Số 4 - Trang 236-242 - 2002
Tytus Bernaś, Jurek Dobrucki
AbstractBackgroundBioreduction of water‐soluble tetrazolium salts (e.g., MTS, XTT, and MTT) to their respective formazans is generally regarded as an indicator of cell “redox activity.” The reaction is attributed mainly to mitochondrial enzymes and electron carriers. However, MTT reduction may also be catalyzed by a number of othe...... hiện toàn bộ
Optimal detection of apoptosis by flow cytometry depends on cell morphology
Wiley - Tập 14 Số 8 - Trang 891-897 - 1993
L. Zamai, Elisabetta Falcieri, Giorgio Zauli, Amelia Cataldi, Marco Vitale
AbstractFlow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a “subdiploid” peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytom...... hiện toàn bộ
Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry
Wiley - Tập 15 Số 1 - Trang 12-20 - 1994
Ingrid Schmid, Christel H. Uíttenbogaart, Janis V. Giorgi
AbstractA rapid, gentle, and sensitive method for quantification of cells undergoing apoptosis is presented. The method allows the simultaneous determination of dual‐color cell surface immunofluorescence. Cells are stained for 7 min with the vital dye Hoechst 33342 (HO342) for identification of live and apoptotic cells. 7‐amino‐actinomycin D (7‐AAD) is added to dis...... hiện toàn bộ
Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity
Wiley - Tập 47 Số 3 - Trang 158-162 - 2002
Csaba Vízler, Tünde Nagy, Erzsébet Kúsz, Hristos Glavinas, E. Duda
AbstractBackgroundFlow‐cytometric assays are convenient alternatives to classic radioactive natural killer (NK) tests. MitoTracker Green FM, a green fluorescent intracellular probe serving originally for staining mitochondria, seemed especially suitable for labeling NK target cells.Materia...... hiện toàn bộ
Identification and quantitation of apoptotic cells following anti‐CD3 activation of murine G0 T cells
Wiley - Tập 14 Số 8 - Trang 883-890 - 1993
Francis J. Chrest, M Buchholz, Young Ho Kim, Taeg‐Kyu Kwon, Albert A. Nordin
AbstractMultiparameter flow cytometry and cell sorting were used to examine the process of apoptosis after activation of murine resting T cells with immobilized anti‐CD3. Activated T cells treated with Hoechst 33342 (HO‐33342) and analyzed by flow cytometry showed two major cell populations of high and low fluorescence. These populations were sorted and the DNA ext...... hiện toàn bộ
Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients
Wiley - Tập 50 Số 6 - Trang 298-304 - 2002
Michael J. Warzynski, David M. Graham, Richard A. Axtell, James V. Higgins, Yuki Hammers
AbstractTen years ago, we made an incidental flow cytometric observation while immunophenotyping biopsy and marrow samples from children suspected to have leukemia/non‐Hodgkin's lymphoma, but were subsequently diagnosed with neuroblastoma. The samples contained neoplastic CD45− cells that had an extremely bright CD56+ (beyo...... hiện toàn bộ
Optical method for measurement of number and shape of attached cells in real time
Wiley - Tập 19 Số 2 - Trang 97-102 - 1995
Jeremy J. Ramsden, Siwei Li, Elmar Heinzle, Jiří E. Přenosil
AbstractA novel, on‐line method of accurately parametrizing the shape of spreading cells is described. The substrate on which the cells are deposited serves as an optical waveguide. The method is based on the interaction between the evanescent part of the guided waves and the cells. No special labelling of the cells is required. A mathematical framework for interpr...... hiện toàn bộ
Rapid quantification of lymphocyte subsets in heterogeneous cell populations by flow cytometry
Wiley - Tập 16 Số 2 - Trang 152-159 - 1994
Klaus Pechhold, Thomas Pohl, Dieter Kabelitz
AbstractDetermination of the number of viable cells or quantification of lymphocyte subsets in heterogeneous cell populations is critically important for cytotoxicity assays, apoptosis assays, or the analysis of differential activation of T‐cell subsets by distinct stimuli. In this report, we describe a rapid flow cytometry method termed Standard Cell Dilution Anal...... hiện toàn bộ
The multivariate prognostic index and nuclear DNA content are independent prognostic factors in primary breast cancer patients
Wiley - Tập 10 Số 1 - Trang 56-61 - 1989
J.C. van der Linden, J. Lindeman, Jan P. A. Baak, Coby Meijer, Chester J. Herman
AbstractThe predictive value of a previously described Multivariate Prognostic Index (which incorporates weighted values of the mitotic activity index, tumor size, and the axillary lymph node status), and the nuclear DNA content (DNA) was evaluated in 156 patients with primary invasive ductal breast cancer, diagnosed between 1980 and 1983. The results were analysed...... hiện toàn bộ
Nucleic acid dyes for detection of apoptosis in live cells
Wiley - Tập 21 Số 3 - Trang 265-274 - 1995
Tom Frey
AbstractApoptotic thymocytes were found to be much dimmer than normal thymocytes when stained with several nucleic acid dyes. These dyes provide a quick and simple assay for apoptosis which works for live cells and does not require a UV laser. The collection of dyes giving this staining pattern includes reagents suitable for use in either the FL1, FL2, or FL3 chann...... hiện toàn bộ
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