Wiley
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Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probesAbstract Background Bioreduction of water‐soluble tetrazolium salts (e.g., MTS, XTT, and MTT) to their respective formazans is generally regarded as an indicator of cell “redox activity.” The reaction is attributed mainly to mitochondrial enzymes and electron carriers. However, MTT reduction may also be catalyzed by a number of othe...
Wiley - Tập 47 Số 4 - Trang 236-242 - 2002
Flow cytometric detection of activated mouse integrin αIIbβ3 with a novel monoclonal antibodyAbstract Background Integrin αIIbβ3 mediates platelet adhesion and aggregation and plays a crucial role in thrombosis and hemostasis. αIIbβ3 is expressed in a low affinity state on resting platelets. Upon platelet activation, αIIbβ3 shifts to a high affinity conformation that efficiently binds its ligands. On human platelets, the hi...
Wiley - Tập 48 Số 2 - Trang 80-86 - 2002
Dual‐laser, differential fluorescence correction method for reducing cellular background autofluorescenceAbstract A method has been developed for reducing the intrinsic autofluorescence background component in cells labeled with fluorescent antibodies, thus permitting low levels of antibody‐binding on highly autofluorescent cells to be quantified. The method is based on the broad autofluorescent excitation spectra compared to the well‐defined spectra of the fluorescent... ... hiện toàn bộ
Wiley - Tập 7 Số 6 - Trang 566-574 - 1986
Dead cell discrimination with 7‐amino‐actinomcin D in combination with dual color immunofluorescence in single laser flow cytometryAbstract Identification of nonviable cells in immunofluorescently stained cell populations is essential for obtaining accurate data. Fluorescent non‐vital DNA dyes, particularly propidium iodide (PI), have been used routinely in flow cytometry for discrimination of dead cells from viable cells on the basis of fluorescence. We describe here the use of an alternative ... ... hiện toàn bộ
Wiley - Tập 13 Số 2 - Trang 204-208 - 1992
Fluorescence energy transfer measurements on cell surfaces: A critical comparison of steady‐state fluorimetric and flow cytometric methodsAbstract The energy transfer efficiency E was measured between fluorescein‐conjugated concanavalin A (Con A) and rhodamine‐conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady‐state fluorimeter methods were compared. The data were found to... ... hiện toàn bộ
Wiley - Tập 5 Số 2 - Trang 210-216 - 1984
Đặc điểm của tế bào theo cơ chế apoptosis được đo bằng lưu lượng tế bào học Dịch bởi AI Tóm tắt Bài tổng quan này mô tả nhiều phương pháp để nhận diện và phân biệt giữa hai cơ chế chết tế bào khác nhau, apoptosis và hoại tử. Đa phần các phương pháp này đã được áp dụng trong các nghiên cứu về apoptosis trong dòng tế bào bạch cầu HL-60 của người bị kích hoạt bởi các chất ức chế DNA topoizomeras I hoặc II, và trong các tế bào tuyến ức của chuột bởi cả chấ... ... hiện toàn bộ
Wiley - Tập 13 Số 8 - Trang 795-808 - 1992
#Apoptosis #necrosis #lưu lượng tế bào học #HL-60 #tế bào tuyến ức #DNA topoizomeras #dấu hiệu sinh hóa #phân biệt tế bào chết #phương pháp phân định tế bào.
Flow cytometric measurements of fluorescence energy transfer using single laser excitationAbstract Flow cytometric energy transfer (FCET) measurements between labeled specific sites of cell surface elements (Szöllosi et al., Cytometry, 5:210–216, 1984) have been extended in a simplified form using a flow cytometer equipped with single excitation beam. This versatile and easily applicable method has several advantages over any nonflow cytometric (i.e., sp... ... hiện toàn bộ
Wiley - Tập 8 Số 2 - Trang 120-128 - 1987
Optimal detection of apoptosis by flow cytometry depends on cell morphologyAbstract Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a “subdiploid” peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytom... ... hiện toàn bộ
Wiley - Tập 14 Số 8 - Trang 891-897 - 1993
Identification and quantitation of apoptotic cells following anti‐CD3 activation of murine G0 T cellsAbstract Multiparameter flow cytometry and cell sorting were used to examine the process of apoptosis after activation of murine resting T cells with immobilized anti‐CD3. Activated T cells treated with Hoechst 33342 (HO‐33342) and analyzed by flow cytometry showed two major cell populations of high and low fluorescence. These populations were sorted and the DNA ext... ... hiện toàn bộ
Wiley - Tập 14 Số 8 - Trang 883-890 - 1993
Flow cytometric enumeration of absolute lymphocyte number in peripheral blood using two parameters of light scatterAbstract A method was developed to measure the absolute lymphocyte count (ALC) of whole blood using the Spectrum III automated flow cytometer. Ninety‐nine samples of human peripheral blood were analysed on the Spectrum and the Coulter Counter S Plus II, to allow for comparison of the two machines. Regression analysis was used to test the extent of agreement between ... ... hiện toàn bộ
Wiley - Tập 6 Số 2 - Trang 172-174 - 1985
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