Wiley

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Optical method for measurement of number and shape of attached cells in real time
Wiley - Tập 19 Số 2 - Trang 97-102 - 1995
Jeremy J. Ramsden, Siwei Li, Elmar Heinzle, Jiří E. Přenosil
AbstractA novel, on‐line method of accurately parametrizing the shape of spreading cells is described. The substrate on which the cells are deposited serves as an optical waveguide. The method is based on the interaction between the evanescent part of the guided waves and the cells. No special labelling of the cells is required. A mathematical framework for interpr...... hiện toàn bộ
Flow cytometry and phytoplankton
Wiley - Tập 10 Số 5 - Trang 511-521 - 1989
David A. Phinney, Terry L. Cucci
AbstractFlow cytometry and sorting are now an important technology in aquatic research. Simultaneous measurements of individual particle cell size, fluorescence, and light scatter properties are directly applicable to current topics in aquatic research. Flow sorting may be employed to obtain subsets of cells for analysis by conventional methods. The manner in which...... hiện toàn bộ
Flow cytometric analysis of cell cycle‐dependent changes in cell thiol level by combining a new laser dye with hoechst 33342
Wiley - Tập 12 Số 2 - Trang 184-187 - 1991
Martin Poot, Terrance J. Kavanagh, Hee Young Kang, Richard P. Haugland, Peter S. Rabinovitch
AbstractBy halogenation of methylfluoresceindiacetate (MFDA) or eosin‐diacetate, two new dyes for cellular thiol compatible with visible laser excitation have become available. These probes circumvent the use of an ultraviolet (UV)‐excitation system as required by bimane‐based dyes and allow combination with probes for other cellular parameters. The thiol dyes atta...... hiện toàn bộ
Analysis of simian virus 40 infection of CV‐1 cells by quantitative two‐color fluorescence with flow cytometry
Wiley - Tập 9 Số 1 - Trang 52-59 - 1988
John M. Lehman, Judith Laffin, James W. Jacobberger, David Fogleman
AbstractQuantitative two‐color fluorescent analysis of Simian virus (SV40) infection of permissive CV‐1 cells was investigated. Analysis included by quantitation of cellular DNA, the early viral tumor (T) antigen with a monoclonal antibody, and late viral (V) antigens with a polyclonal antibody. T antigen was detected in all phases of the cell cycle at 6 and 12 h, ...... hiện toàn bộ
Dead cell discrimination with 7‐amino‐actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry
Wiley - Tập 13 Số 2 - Trang 204-208 - 1992
Ingrid Schmid, Wanda J. Krall, Christel H. Uíttenbogaart, Jonathan Braun, Janis V. Giorgi
AbstractIdentification of nonviable cells in immunofluorescently stained cell populations is essential for obtaining accurate data. Fluorescent non‐vital DNA dyes, particularly propidium iodide (PI), have been used routinely in flow cytometry for discrimination of dead cells from viable cells on the basis of fluorescence. We describe here the use of an alternative ...... hiện toàn bộ
Flow cytometric detection of the mitochondrial BCL‐2 protein in normal and neoplastic human lymphoid cells
Wiley - Tập 13 Số 5 - Trang 502-509 - 1992
A Aiello, Domenico Delia, Maria Grazia Borrello, D Biassoni, Roberto Giardini, Enrico Fontanella, Francesco Pezzella, Karen Pulford, Marco A. Pierotti, Giuseppe Della Porta
AbstractThe bcl‐2 proto‐oncogene, rearranged and deregulated in B‐cell lymphomas bearing the t(14;18) translocation, encodes an inner mitochondrial membrane protein that blocks apoptotic cell death. We have developed a sensitive immunofluorescence assay for the single‐ and multicolor flow cytometric analysis of bcl‐2 protein in relation to other markers and cell cy...... hiện toàn bộ
Flow cytometric enumeration of absolute lymphocyte number in peripheral blood using two parameters of light scatter
Wiley - Tập 6 Số 2 - Trang 172-174 - 1985
Y. Cheng Smart, Josephine H. Cox, Brian C. Murphy, Arno Enno, Robert C. Burton
AbstractA method was developed to measure the absolute lymphocyte count (ALC) of whole blood using the Spectrum III automated flow cytometer. Ninety‐nine samples of human peripheral blood were analysed on the Spectrum and the Coulter Counter S Plus II, to allow for comparison of the two machines. Regression analysis was used to test the extent of agreement between ...... hiện toàn bộ
Analysis of radiation‐induced apoptosis in human lymphocytes: Flow cytometry using Annexin V and propidium iodide versus the neutral comet assay
Wiley - Tập 48 Số 1 - Trang 14-19 - 2002
Ruth Wilkins, B. C. Kutzner, Mai Thy Truong, J Sanchez-Dardon, J. C. Scaiano
AbstractBackgroundThe neutral comet assay was devised to measure double‐stranded DNA breaks, but it has also been used to measure apoptosis based on its characteristic DNA fragmentation patterns. There is still uncertainty about the reliability of this method. By comparing the comet assay with a flow cytometry method that uses Ann...... hiện toàn bộ
Mitochondrial and nonmitochondrial reduction of MTT: Interaction of MTT with TMRE, JC‐1, and NAO mitochondrial fluorescent probes
Wiley - Tập 47 Số 4 - Trang 236-242 - 2002
Tytus Bernaś, Jurek Dobrucki
AbstractBackgroundBioreduction of water‐soluble tetrazolium salts (e.g., MTS, XTT, and MTT) to their respective formazans is generally regarded as an indicator of cell “redox activity.” The reaction is attributed mainly to mitochondrial enzymes and electron carriers. However, MTT reduction may also be catalyzed by a number of othe...... hiện toàn bộ
Optimal detection of apoptosis by flow cytometry depends on cell morphology
Wiley - Tập 14 Số 8 - Trang 891-897 - 1993
L. Zamai, Elisabetta Falcieri, Giorgio Zauli, Amelia Cataldi, Marco Vitale
AbstractFlow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a “subdiploid” peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytom...... hiện toàn bộ
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