Flow cytometric enumeration of absolute lymphocyte number in peripheral blood using two parameters of light scatter

Wiley - Tập 6 Số 2 - Trang 172-174 - 1985
Y. Cheng Smart1, Josephine H. Cox1, Brian C. Murphy1, Arno Enno2, Robert C. Burton1
1Discipline of Surgical Science, Faculty of Medicine, University of Newcastle, New South Wales, Australia, 2308
2Haematology Department, Royal Newcastle Hospital, Newcastle, New South Wales, Australia, 2300

Tóm tắt

AbstractA method was developed to measure the absolute lymphocyte count (ALC) of whole blood using the Spectrum III automated flow cytometer. Ninety‐nine samples of human peripheral blood were analysed on the Spectrum and the Coulter Counter S Plus II, to allow for comparison of the two machines. Regression analysis was used to test the extent of agreement between the sets of measurements on the two machines. The results demonstrated that the slope of the regression line was not significantly different from one, indicating a high level of correlation between Spectrum and Coulter ALC's. However, the mean difference between Coulter and Spectrum ALC's was not equal to zero, with the Spectrum giving counts approximately 10% lower than those of the Coulter machine. This is attributed to the different ways by which the two machines define a lymphocyte, the Spectrum III by two parameters of light scatter and the Coulter S Plus II by the single parameter of cell volume.

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Tài liệu tham khảo

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10.1136/jcp.35.11.1194

Goldstein G, 1982, Monoclonal antibodies in Clinical Medicine, 39

Hansen WP, 1982, Light scatter as an adjunct to cellular immunofluorescence in flow cytometric systems, J Clin Immunol, 2, 32S

10.1073/pnas.77.8.4914

Smart YC, 1984, T cell subsets and renal allograft rejection, Transplant Proc, 16, 1007

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Wiley

Tập 6 Số 2

172-174

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