Optimal detection of apoptosis by flow cytometry depends on cell morphology

Wiley - Tập 14 Số 8 - Trang 891-897 - 1993
L. Zamai1, Elisabetta Falcieri2, Giorgio Zauli3, Amelia Cataldi4, Marco Vitale5,2,6
1Laboratorio Biologia Cellulare e Microscopia Elettronica, Istituto Ortopedico Rizzoli, Bologna
2Ist. Citomorfologia N.P. C.N.R. c/o Istituto di Ricerca Codivilla‐Putti, Bologna
3Istituto Anatomia Umana Normale, University of Ferrara, Italy
4Istituto Morfologia Umana Normale, University of Chieti, Bologna
5Dipartimento Scienze Biomediche e Biotecnologi, sez. Anatomia Umana, University of Brescia, Italy
6Istituto di Anatoa Umana Normale, University of Bologna, via Irnerio 48, 40126 Bologna, Italy

Tóm tắt

AbstractFlow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a “subdiploid” peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non‐apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells. © 1993 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

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Thông tin xuất bản

Wiley

Tập 14 Số 8

891-897

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