Modified histogram subtraction technique for analysis of flow cytometry data

Wiley - Tập 9 Số 6 - Trang 619-626 - 1988
W. Roy Overton1
1Clinical Immunology Services, Program Resources, Inc., Frederick Cancer Research Facility, Maryland 21701.

Tóm tắt

AbstractAnalysis of flow cytometry histogram data by the subjective selection of an integration window can be a tedious and time‐consuming task and is often inaccurate. A new method for automated calculation of the percent positive from immunofluorescence histograms is presented. This new method is a modification of the currently used method of channel‐by‐channel histogram subtraction. Its accuracy is compared to that of the channel‐by‐channel histogram subtraction method and to another currently used automated method, which selects an integration window by finding the channels that contain the most fluorescent 2% of a control histogram.The new histogram subtraction method is objective, easy to use, and is more accurate than other currently used automated analysis methods. PASCAL source code is given for each method of analysis.

Từ khóa


Tài liệu tham khảo

10.1109/PROC.1969.7447

Loar LD, 1986, Production and characterization of monoclonal antibodies to rabbit lymphocyte subpopulations; I. Tissue immunofluorescence and flow cytometric analysis, J Immunol, 137, 2784, 10.4049/jimmunol.137.9.2784

10.1002/cyto.990040111

Scher I, 1980, Development of B lymphocytes in the mouse; studies of frequency and distribution of surface IgM and IgD in normal and immune‐defective CBA/N F1 mice, J Immunol, 125, 1686, 10.4049/jimmunol.125.4.1686

10.1177/28.2.7354211

10.1007/BF00915224

Thông tin
Thông tin xuất bản

Wiley

Tập 9 Số 6

619-626

Thông tin tác giả