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Characterization of propionic acid bacteria using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry
Springer Science and Business Media LLC - - 2011
L. I. Vorob’eva, F. M. Khasaeva, N. V. Vasilyuk, E. Trenquil
It was shown that matrix-assisted laser desorption/ionization (MALDI) mass spectrometry could be used for the diagnostic characterization of propionic acid bacteria (PABs). The spectra of proteins (whole PAB cells) with a molecular mass of 3000 to 11 000 were obtained and analyzed using three matrices: sinapinic (SA), 2,5-dihydroxibenzoic (DHB), and α-cyano-4-hydroxycinnamic acid (HCCA). The MALDI...... hiện toàn bộ
Microbial Community Composition as an Indicator of the State of Basins Located at the Sea Coast (Exemplified by the Kanda Bay, Kandalaksha Gulf, White Sea)
Springer Science and Business Media LLC - Tập 92 - Trang 819-830 - 2023
A. S. Savvichev, N. A. Demidenko, V. V. Kadnikov, V. V. Belenkova, I. I. Rusanov, V. M. Gorlenko
Formation of the Kanda Bay resulted from construction of a railway dam (1916) and subsequent isolation of the sea lagoon from the main basin of the Kandalaksha Gulf, White Sea. Decreased action of tidal flows, which mix the water column of the lagoon, altered the hydrological regime of the basin. Decreased water exchange resulted in formation of oxygen-depleted near-bottom water and to sulfide con...... hiện toàn bộ
Regulatory aspects of ectoine biosynthesis in halophilic bacteria
Springer Science and Business Media LLC - Tập 79 - Trang 583-592 - 2010
I. I. Mustakhimov, A. S. Reshetnikov, V. N. Khmelenina, Yu. A. Trotsenko
In this review, the current concepts of metabolic and genetic regulation of the synthesis of ectoine and hydroxyectoine, widely distributed osmoprotectors of halophilic bacteria, are analyzed and generalized. Due to their water-retaining properties, these compounds can be used as multifunctional bioprotectants. The relevance of deciphering ectoine biosynthesis regulation at the levels of enzyme ac...... hiện toàn bộ
Anhydrobiosis in yeast: Stabilization by exogenous lactose
Springer Science and Business Media LLC - Tập 78 - Trang 624-629 - 2009
A. I. Rapoport, G. M. Khroustalyova, L. M. Crowe, J. H. Crowe
We have found that incubation in lactose solutions (0.75 M) of yeast culture Saccharomyces cerevisiae sensitive to dehydration damage increased the stability of the cells during dehydration. Simultaneously with this increase in viability, a decrease in plasma membrane permeability during rehydration was seen. Using Fourier transform infrared spectroscopy to measure lipid phase transitions, we obse...... hiện toàn bộ
Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245
Springer Science and Business Media LLC - - 2023
L. N. Petrova, С. С. Евстигнеева, Yulia A. Filip’echeva, I. V. Volokhina, Gennady L. Burygin, L. Yu. Matora, А. В. Шелудько
Molecular Genetic and Functional Analysis of the Conjugation System of the pBS72 Plasmid from Bacillus subtilis Environmental Isolates
Springer Science and Business Media LLC - - 2022
А. С. Гуринович, М. А. Титок
Diversity and dynamics of bacteriophages in horse feces
Springer Science and Business Media LLC - Tập 76 - Trang 236-242 - 2007
E. E. Kulikov, A. S. Isaeva, A. S. Rotkina, A. A. Manykin, A. V. Letarov
The complex cellulolytic microbial community of the horse intestines is a convenient model for studying the ecology of bacteriophages in natural habitats. Unlike the rumen of the ruminants, this community of the equine large intestine is not subjected to digestion. The inner conditions of the horse gut are much more stable in comparison to other mammals, due to the fact that the horse diet remains...... hiện toàn bộ
Primary characterization of dominant cell surface proteins of halotolerant methanotroph Methylomicrobium alcaliphilum 20Z
Springer Science and Business Media LLC - Tập 80 - Trang 608-618 - 2011
V. N. Shchukin, V. N. Khmelenina, B. Ts. Eshinimayev, N. E. Suzina, Yu. A. Trotsenko
Cell surface-associated proteins with molecular masses of 27 and 80 kDa found in Methylomicrobium alcaliphilum were studied. The MEALZv2_1030034 and MEALZv2_1030035 genes encoding these proteins were identified in the partially annotated genome of the methanotroph. Polypeptides MEALZv2_1030034 and MEALZv2_1030035 showed high homology (>50% identity) with the surface proteins CorA and CorB of Methy...... hiện toàn bộ
Molecular analysis of high-affinity methane-oxidizing enrichment cultures isolated from a forest biocenosis and agrocenoses
Springer Science and Business Media LLC - - 2010
I. K. Kravchenko, A. K. Kizilova, S. A. Bykova, E. V. Men’ko, V.F. Gal’chenko
Methane oxidation by microorganisms inhabiting aerobic soils is a key process involved in the regulation of the concentration of this significant greenhouse gas in the atmosphere; however, the microorganisms responsible for this process remain unknown. Three stable methane-oxidizing cultures were isolated from samples of forest soils (FS) and agricultural soils (AS) of Moscow oblast, as well as fr...... hiện toàn bộ
Biochemical Characterization of the Yeast Yarrowia lipolytica Overproducing Carboxylic Acids from Ethanol: Nitrogen Metabolism Enzymes
Springer Science and Business Media LLC - Tập 72 - Trang 418-422 - 2003
A. P. Il'chenko, O. G. Chernyavskaya, N. V. Shishkanova, T. V. Finogenova
A comparative assay of nitrogen metabolism enzymes in the Yarrowia lipolytica mutant N1 grown under conditions promoting the overproduction of either α-ketoglutaric acid (KGA) or citric acid showed that the overproduction of KGA correlates with an increase in the activities of the NAD- and NADP-linked glutamate dehydrogenase, glutamic–pyruvic transaminase, and glutamic–oxaloacetic transaminase rea...... hiện toàn bộ
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