Biochemical Characterization of the Yeast Yarrowia lipolytica Overproducing Carboxylic Acids from Ethanol: Nitrogen Metabolism Enzymes

Springer Science and Business Media LLC - Tập 72 - Trang 418-422 - 2003
A. P. Il'chenko1, O. G. Chernyavskaya1, N. V. Shishkanova1, T. V. Finogenova1
1Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia

Tóm tắt

A comparative assay of nitrogen metabolism enzymes in the Yarrowia lipolytica mutant N1 grown under conditions promoting the overproduction of either α-ketoglutaric acid (KGA) or citric acid showed that the overproduction of KGA correlates with an increase in the activities of the NAD- and NADP-linked glutamate dehydrogenase, glutamic–pyruvic transaminase, and glutamic–oxaloacetic transaminase reactions. These reactions are likely to be responsible for the overproduction of KGA by this mutant. In contrast, the overproduction of citric acid correlated with a decline in the activities of the NAD- and NADP-linked glutamate dehydrogenases and with an increase in the activities of glutamine synthetase and glutamate synthase.

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Tài liệu tham khảo

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Springer Science and Business Media LLC

Tập 72

418-422

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