Characterization of propionic acid bacteria using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry
Tóm tắt
It was shown that matrix-assisted laser desorption/ionization (MALDI) mass spectrometry could be used for the diagnostic characterization of propionic acid bacteria (PABs). The spectra of proteins (whole PAB cells) with a molecular mass of 3000 to 11 000 were obtained and analyzed using three matrices: sinapinic (SA), 2,5-dihydroxibenzoic (DHB), and α-cyano-4-hydroxycinnamic acid (HCCA). The MALDI spectra of PAB revealed the protein peaks characteristic of (1) the genus Propionibacterium (3496, 5386, 5605, 10 470), (2) the groups of species sharing the common composition of their cell walls and fatty acids, and (3) a species (four species were investigated). Exemplified by the P. shermanii strains (the collection and mutant ones) producing and not producing vitamin B12, the possibility of using MALDI profiles for strain differentiation was confirmed. The MALDI profiles of the propionic acid cocci of the genus Luteococcus differ substantially from the profiles of PAB strains of the genus Propionibacterium, which is an additional proof of the validity of whole-cell MALDI spectra for generic differentiation of bacteria. Our investigation shows that the bacterial groups determined using the MALDI profiles correlate with the phylogenetic 16S rRNA gene groups, thus demonstrating the high resolution of this method for the differentiation of intraspecific differences (subspecies, strains).
Từ khóa
Tài liệu tham khảo
Anhalt, J.P. and Fenselau, C., Identification of Bacteria Using Mass Spectrometry, Anal. Chem., 1975, vol. 47, pp. 219–225.
Holland, R.D., Wilkes, J.G., Rafii, F., Sutherland, J.B., Persons, C.C., Voorhees K.J., and Lay, J.O., Jr., Rapid Identification of Intact Whole Bacteria Based on Spectral Patterns Using Matrix-Assisted Lazer Desorbtion/Ionization with Time-of-Flight Mass Spectrometry, Rapid Commun. Mass Spectrom., 1996, vol. 10, pp. 1227–1232.
Claydon, M.A., Davey, S.N., Edwards-Jones, V., and Gordon, D.B., The Rapid Identification of Intact Microorganisms Using Mass Spectrometry, Nature Biotechnol., 1996, vol. 10, pp. 1992–1996.
Welham, K., Domin, M., Scannel, D., Cohen, E., and Ashton, D., The Characterization of Microorganisms by Matrix-Assisted Lazer Desorbtion/Ionization Time-of-Flight Mass Spectrometry Rapid Commun. Mass Spectrom., 1998, vol. 12, pp. 176–180.
Liyanage, R. and Lay, J.O. Jr., An Introduction to MALDI-TOF MS, in Identification of Microorganisms by Mass Spectrometry, Wilkins, Ch. and Lay, J.O. Jr., Eds., New Jersey: Wiley, 2006, pp. 39–60.
Jones, J.J., Stump, M.J., Fleming, R.C., Lay, J.O., and Wilkins, C.L., Investigation of MALDI-TOF and FTMS Techniques for Analysis of Escherichia coli Whole Cells, Anal. Chem., 2003, vol. 75, pp. 1340–1347.
Wahl, K.L. and Valentine, N.B., Analysis of Whole Bacterial Cells by Flow Field-Flow Fractionation and Matrix-Assisted Lazer Desorption/Ionization Time-of-Flight Mass Spectrometry, Anal. Chem., 2003, vol. 75, pp. 2746–2752.
Sauer, S. and Kliem, M., Mass Spectrometry Tools for the Classification and Identification of Bacteria, Nature Rev. Microbiol., 2010, vol. 8, no. 81, pp. 74–81.
Vargha, M., Takats, Z., Konopka, A., and Nakatsu, C.H., Optimization of MALDI-TOF MS for Strain Level Differentiation of Arthrobacter Isolates, Microbiol. Meth., 2006, vol. 66, pp. 399–409.
Dickinson, D.N., La Duc, M.T., Haskins, W.E., Gornishkin, I., Winefordner, J.D., Powel D.H., and Venkateswaran K., Species Differentiation of a Diverse Suite of Bacillus Spores by Mass Spectrometry-Based Protein Profiling, Appl. Environ. Microbiol. 2004, vol. 70, pp. 475–482.
Hettick, J.M., Kashon, M.L., Simpson, J.P., Siegel, P.D., Mazurek, G.H., and Weissman, D.N., Proteomic Profiling of Intact Mycobacteria by Matrix-Assisted Lazer Desorption/Ionization Time-Of-Flight Mass Spectrometry, Anal. Chem., 2004, vol. 76, pp. 5769–5776.
Dieckman, R., Graeber, I., Kaesler, I., Szewzyk, U., and von Dohren, H. Rapid Screening and Dereplication of Bacteria Isolates from Marine Sponges of the Sula Ridge by Intact-Cell-MALDI-TOF Mass Spectrometry, Appl. Microbiol. Biotechnol., 2005, vol. 67, pp. 539.
Vorob’eva, L.I., Propionovokislye bakterii (Propionic Acid Bacteria), Moscow: Mosk. Gos. Univ., 1995.
Smole, S.C., Ring, L.A., Leopold, P.E., and Arbeit, R.D., Sample Preparation of Gram-Positive Bacteria for Identification by Matrix-Assisted Lazer Desorption/Ionization Time-Of-Flight Mass Spectrometry, J. Microbiol. Methods., 2002, vol. 48, pp. 107–115.
Evason, D.J., Claydon, M.A., and Gordon, D.B., Exploring the Limits of Bacterial Identification by Intact Cell-Mass Spectrometry, J. Amer. Soc. Mass Spectrom., 2001, vol. 12, pp. 49–54.
Williams, T.L., Andrzejewski, D., Lay, J.O., and Musser, S.M., Experimental Factors Affecting the Quality and Reproducibility of MALDI-TOF Mass Spectra Obtained from Whole Bacteria Cells, J. Amer. Mass Spectrom., 2003, vol. 14, pp. 342–351.
Cummins, C.S. and Johnson, J.L., The genus Propionibacterium, in The Procaryotes, Ballows, A. Trüper, H.G., Dworkin, M., Harder, W., and Schleifer, K.-H., Eds., vol. 1, ch. 37, New York: Springer, 1992, pp. 834–849.
Vorobjeva, L.I., Propionibacteria, Dordrecht: Kluwer Academic, 1999.
Van Nieuwholtz, J., A Taxonomic Re-Evaluation of Propionibacterium coccoides, Ph. D. Thesis, Dep. Microbiol. Biochem., University of the Orange Free State, Bloemfontein, South Africa. 1998.
Arnold, R.J. and Reilly, J.P. Fingerprint Matching of Escherichia coli Strains with Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry of Whole Cells Using a Modified Correlation Approach, Rapid Commun. Mass Spectrom., 1998, vol. 12, pp. 630–636.
Edwards-Jones, V., Claydon, M.A., Evason D.J., Walker, J., Fox, A.J., and Gordon, D.B., Rapid Discrimination between Methicilline-Sensitive and Methicilline-Resistant Staphylococcus aureus by Intact Cell Mass Spectrometry, J. Med. Microbiol., 2000, vol. 49, pp. 295–300.
Vorob’eva, L.I., Tourova, T.P., Kraeva, N.I., and Alekseeva, M.A., Propionic Acid Bacteria and Their Taxonomic Position, Mikrobiologiya, 1983, vol. 52, no. 3, pp. 465–471.
Namura, T., Nakeuchi, M., and Yokota, A. Luteococcus japonicus gen nov., sp.nov., a New Gram-Positive Coccus with LL-Diaminopimelic Acid in the Cell Wall, Int.J. Syst. Bacteriol., 1994, vol. 44, pp. 348–356.