Microbiology and Immunology
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Comparison of Virulence between Seoul Virus Strain SR‐11 and Hantaan Virus Strain 76–118 of Hantaviruses in Newborn Mice
Microbiology and Immunology - Tập 37 Số 7 - Trang 557-562 - 1993
AbstractVirulence of hantavirus strain of SR‐11 Seoul virus and Hantaan 76–118
(HTN) of Hantaan virus were compared. Infections of both strains were lethal in
newborn mice. However, inoculum required to cause lethal infection was about
4,000 times higher for strain HTN (1.65 × 103 PFU/mouse/LD50) than for strain
SR‐11 (0.36 PFU). Thus, both strains were considered pathogenic to newborn mice
but th...
Anti‐inflammatory properties of intestinal Bifidobacterium strains isolated from healthy infants
Microbiology and Immunology - Tập 56 Số 1 - Trang 27-39 - 2012
ABSTRACTCertain Bifidobacterium strains have been shown to inhibit inflammatory
responses in intestinal epithelial cells. However, the precise mechanisms of
these effects, including the chemical nature of the active compounds, remain to
be elucidated. Here partial characterization of the anti‐inflammatory properties
of Bifidobacterium strains isolated from feces of healthy infants is reported.
It ...
Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction
Microbiology and Immunology - Tập 56 Số 9 - Trang 630-638 - 2012
ABSTRACTGroup A rotaviruses (RVA) are a major cause of acute infantile
gastroenteritis. The viral genome comprises 11 double‐stranded RNA segments and
the respective gene segments are classified into more than eight genotypes,
according to the nucleotide sequence similarities. So far, it has been difficult
to amplify full‐length sequences of long RNA segments of rotaviruses by one‐time
only RT‐PCR...
Detection of antibodies against Fusobacterium necrophorum and Porphyromonas levii-like species in dairy cattle with papillomatous digital dermatitis
Microbiology and Immunology - Tập 54 Số 6 - Trang 338-346
Detection and Genetical Characterization of Shiga Toxin‐Producing Escherichia coli from Wild Deer
Microbiology and Immunology - Tập 42 Số 12 - Trang 815-822 - 1998
AbstractShiga toxin (Stx)‐producing Escherichia coli (STEC) strains isolated
from wild deer in Japan were examined. A total of 43 fecal samples were
collected 4 times from 4 different sites around Obihiro City, Hokkaido, Japan,
in June and July 1997. Seven STEC strains were isolated by PCR screening, all of
them were confirmed by ELISA and Vero cell cytotoxicity assay to be producing
only active S...
Rapid detection and quantification of Ebola Zaire virus by one‐step real‐time quantitative reverse transcription‐polymerase chain reaction
Microbiology and Immunology - Tập 61 Số 3-4 - Trang 130-137 - 2017
ABSTRACTGiven that Ebola virus causes severe hemorrhagic fever in humans with
mortality rates as high as 90%, rapid and accurate detection of this virus is
essential both for controlling infection and preventing further transmission.
Here, a one‐step qRT‐PCR assay for rapid and quantitative detection of an Ebola
Zaire strain using GP, VP24 or VP40 genes as a target is introduced. Routine
assay con...
Maternal Transmission and Dental Caries Induction in Sprague‐Dawley Rats Infected with Streptococcus mutans
Microbiology and Immunology - Tập 32 Số 8 - Trang 785-794 - 1988
AbstractThirty‐four female rats (18 days old) were infected with Streptococcus
mutans MT8148R (serotype c) or 6715 (g). Diets containing different proportions
of sucrose were used to prepare the dams which harbored various levels of S.
mutans in their oral cavity. Around 66 days of age, the female rats were bred
and 34 dams subsequently bore 322 offspring. The dams were killed upon weaning
(20 day...
An Evaluation of Serodiagnostic Tests in Patients with Candidemia: Beta‐Glucan, Mannan, Candida Antigen by Cand‐Tec and D‐Arabinitol
Microbiology and Immunology - Tập 37 Số 3 - Trang 207-212 - 1993
AbstractThe serodiagnostic tests, beta‐glucan, mannan, candida antigen by
Cand‐Tec, and D‐arabinitol were evaluated in 10 patients with candidemia, 14
patients with suspected fungemia, and 10 healthy persons. By blood culture or
lysis centrifugation, C. albicans was isolated from 5 patients, C. parapsilosis
from 4, and C. tropicalis from 1 patient; no organisms were isolated from the 14
patients w...
Kinetics of Anti‐Mannan Antibodies Useful in Confirming Invasive Candidiasis in Immunocompromised Patients
Microbiology and Immunology - Tập 40 Số 2 - Trang 125-131 - 1996
AbstractIn studying the anti‐mannan antibodies longitudinally in serial serum
samples of three immunocompromised patients, it was observed that anti‐mannan
antibodies started to increase shortly after the moment that cultures of
deep‐tissue sites became positive with Candida albicans. The mean anti‐mannan
antibody titers determined in a group of 36 immunocompromised patients with
invasive candidia...
The Relationship between Microbiota and Polyamine Concentration in the Human Intestine: A Pilot Study
Microbiology and Immunology - Tập 51 Số 1 - Trang 25-35 - 2007
AbstractThe fecal microbiota of 10 hospitalized elderly subjects and 14 healthy
adults were analyzed by terminal‐restriction fragment length polymorphism
(T‐RFLP) analysis using HhaI, MspI, HaeIII, and AluI, as well as fecal polyamine
(PA) concentration. The T‐RFLP profiles of the fecal microbiota of the subjects
were roughly divided into 2 clusters—I (9 out of 11 were derived from
hospitalized el...
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