Anti‐inflammatory properties of intestinal Bifidobacterium strains isolated from healthy infants

Microbiology and Immunology - Tập 56 Số 1 - Trang 27-39 - 2012
Ekaterina V. Khokhlova1, Smeianov Vv, Б. А. Ефимов, Л. И. Кафарская, С. И. Павлова, Andrey N. Shkoporov
1Department of Microbiology and Virology, Russian State Medical University, Moscow, Russia.

Tóm tắt

ABSTRACTCertain Bifidobacterium strains have been shown to inhibit inflammatory responses in intestinal epithelial cells. However, the precise mechanisms of these effects, including the chemical nature of the active compounds, remain to be elucidated. Here partial characterization of the anti‐inflammatory properties of Bifidobacterium strains isolated from feces of healthy infants is reported. It was found that conditioned media (CM) of all strains studied are capable of attenuating tumor necrosis factor‐α (TNF‐α) and lipopolysaccharide‐ (LPS) induced inflammatory responses in the HT‐29 cell line. In contrast, neither killed bifidobacterial cells, nor cell‐free extracts showed such activities. Further investigations resulted in attribution of this activity to heat‐stable, non‐lipophilic compound(s) resistant to protease and nuclease treatments and of molecular weight less than 3  kDa. The anti‐inflammatory effects were dose‐ and time‐dependent and associated with inhibition of IκB phosphorylation and nuclear factor‐κ light chain enhancer of activated B cells (NF‐κB)‐dependent promoter activation. The combined treatments of cells with CMs and either LPS or TNF‐α, but not with CMs alone, resulted in upregulation of transforming growth factor‐β1, IκBζ, and p21CIP mRNAs. Our data suggest certain species‐specificities of the anti‐inflammatory properties of bifidobacteria. This observation should prompt additional validation studies using larger set of strains and employing the tools of comparative genomics.

Từ khóa


Tài liệu tham khảo

10.1111/j.1574-6941.2006.00182.x

10.1542/peds.2005-2824

10.1038/nature08821

10.1271/bbb1961.49.13

Kim J.E., 2007, Cancer chemopreventive effects of lactic acid bacteria, J Microbiol Biotechnol, 17, 1227

10.1128/AEM.01763-06

10.1007/10_2008_097

10.1038/nature09646

10.1136/gut.53.1.108

10.1136/gut.2004.044834

10.1038/ajg.2008.118

10.1111/j.1365-2567.2006.02358.x

10.3748/wjg.v12.i23.3729

10.1371/journal.pone.0005184

10.1053/j.gastro.2004.09.001

10.1136/gut.2003.026252

10.3748/wjg.14.2511

10.1128/IAI.00119-07

10.1002/ibd.21057

10.1111/j.1365-2249.2007.03522.x

10.1016/S0168-1605(98)00197-4

10.1111/j.1348-0421.2002.tb02765.x

10.1016/j.ijfoodmicro.2009.12.023

10.1128/CDLI.11.4.686-690.2004

10.1128/AEM.03127-09

Sambrook J., 2001, Molecular Cloning: A Laboratory Manual

10.1002/ibd.21262

10.1007/s005350050216

10.1038/77783

10.1126/science.288.5474.2222

10.1126/science.291.5505.881

10.1136/gut.2004.054098

10.4049/jimmunol.174.6.3237

10.1186/1471-2164-8-215

10.1152/ajpgi.00341.2003

Park M.S., 2007, Assessment of lipopolysaccharide‐binding activity of Bifidobacterium and its relationship with cell surface hydrophobicity, autoaggregation, and inhibition of interleukin‐8 production, J Microbiol Biotechnol, 17, 1120

10.1079/BJN20051681

10.1016/j.jaci.2005.10.043

10.1152/ajpgi.90227.2008

10.3748/wjg.v10.i3.455

10.1186/1471-2172-10-54

10.1203/PDR.0b013e31809fd85e

10.1093/jn/136.6.1483

10.1126/science.289.5484.1560

10.4049/jimmunol.176.2.1228

10.1038/ni1018

10.1371/journal.pone.0004365

10.1074/jbc.M511956200

10.1038/labinvest.3700669

10.1136/gut.47.1.79

10.1016/j.ijfoodmicro.2010.09.007

Thông tin
Thông tin xuất bản

Microbiology and Immunology

Tập 56 Số 1

27-39

Thông tin tác giả