Journal of Microscopy
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3D imaging of cement‐based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioningSummary In this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z‐stacks, which are then aligned and stitched based on phase correlation. The method can... ...
Journal of Microscopy - Tập 258 Số 2 - Trang 151-169 - 2015
The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopySUMMARY Two methods of freezing samples for freeze‐fracture electron microscopy have been compared using X‐ray diffraction and freeze‐fracture results from lipid‐water model systems. Perturbations of the molecular organization of hydrocarbon chains and the extent of ice crystal formation have been evaluated for lamellar phases of egg lecithin containing 16% water an... ...
Journal of Microscopy - Tập 112 Số 1 - Trang 103-113 - 1978
Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopyWe introduce two‐photon image correlation spectroscopy (ICS) using a video rate capable multiphoton microscope. We demonstrate how video rate two‐photon microscopic imaging and image correlation analysis may be combined to measure molecular transport properties over ranges typical of biomolecules in membrane environments. Using two‐photon ICS, we measured diffusion coefficients as large as... ...
Journal of Microscopy - Tập 200 Số 1 - Trang 14-25 - 2000
An investigation into microstructure and particle distribution of Ni–P/diamond composite thin filmsThe present paper deals with a theoretical and experimental study of the co‐deposition of spheroidal particles in a metal matrix deposited by autocatalytic chemical deposition (ACD). The 3‐D particle size distribution — as measured by image analysis of SEM micrographs of metallographic cross‐sections — is related to the size distribution of the starting powder — as measured by photon autoc... ...
Journal of Microscopy - Tập 185 Số 2 - Trang 283-291 - 1997
A new preparation method to study fresh plant structures with X‐ray computed tomographySummary Since the development of X‐ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good‐quality images. Therefore, we present a new preparation method for fresh material which incl... ...
Journal of Microscopy - Tập 233 Số 1 - Trang 1-4 - 2009
Imaging applications of synchrotron X‐ray phase‐contrast microtomography in biological morphology and biomaterials science. I. General aspects of the technique and its advantages in the analysis of millimetre‐sized arthropod structureSummary Synchrotron‐generated X‐rays provide scientists with a multitude of investigative techniques well suited for the analysis of the composition and structure of all types of materials and specimens. Here, we describe the properties of synchrotron‐generated X‐rays and the advantages that they provide for qualitative morphological research of millimetre‐sized bio... ...
Journal of Microscopy - Tập 227 Số 1 - Trang 51-71 - 2007
Porphyrin‐related photosensitizers for cancer imaging and therapeutic applicationsSummary A photosensitizer is defined as a chemical entity, which upon absorption of light induces a chemical or physical alteration of another chemical entity. Some photosensitizers are utilized therapeutically such as in photodynamic therapy (PDT) and for diagnosis of cancer (fluorescence diagnosis, FD). PDT is approved for several cancer indications and FD has rec... ...
Journal of Microscopy - Tập 218 Số 2 - Trang 133-147 - 2005
Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samplesSUMMARY The preparation and high resolution observation of frozen hydrated thin sections has been studied by transmission electron microscopy (TEM and STEM) on model systems, including pure water, protein solutions, catalase crystals, myelin sheath and various tissues. The state of the ice is determined by electron diffraction. Mass measurement in the electron micro... ...
Journal of Microscopy - Tập 131 Số 1 - Trang 1-9 - 1983
Molecular cryo‐electron tomography of vitreous tissue sections: current challengesSummary Electron tomography of vitreous tissue sections (tissue TOVIS) allows the study of the three‐dimensional structure of molecular complexes in a near‐native cellular context. Its usage is, however, limited by an unfortunate combination of noisy and incomplete data, by a technically demanding sample preparation procedure, and by a disposition for specimen degra... ...
Journal of Microscopy - Tập 235 Số 3 - Trang 293-307 - 2009
Visualizing filamentous actin on lipid bilayers by atomic force microscopy in solutionThe surface structure of actin filaments (F‐actin) was visualized at high resolution, by atomic force microscopy (AFM) in aqueous solution, in large paracrystals prepared on positively charged lipid monolayers. The increased stability of these closely packed specimens allowed us to show that both the long pitch (38 nm) and the monomer (5.8 nm) can be directly resolved by AFM in the contact... ...
Journal of Microscopy - Tập 201 Số 3 - Trang 377-382 - 2001
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