Journal of Microscopy

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A new preparation method to study fresh plant structures with X‐ray computed tomography
Journal of Microscopy - Tập 233 Số 1 - Trang 1-4 - 2009
Olivier Leroux, Manfred Schlosser, E. Bellefroid, Myriam Claeys, Marjolein Couvreur, Gaëtan Borgonie, Luc Van Hoorebeke, Bert Masschaele, Ronald Viane
SummarySince the development of X‐ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good‐quality images. Therefore, we present a new preparation method for fresh material which incl...... hiện toàn bộ
Imaging applications of synchrotron X‐ray phase‐contrast microtomography in biological morphology and biomaterials science. I. General aspects of the technique and its advantages in the analysis of millimetre‐sized arthropod structure
Journal of Microscopy - Tập 227 Số 1 - Trang 51-71 - 2007
Oliver Betz, Ulrike G. K. Wegst, Daniela Weide, Michael Heethoff, Lukas Helfen, Wah-Keat Lee, Peter Cloetens
SummarySynchrotron‐generated X‐rays provide scientists with a multitude of investigative techniques well suited for the analysis of the composition and structure of all types of materials and specimens. Here, we describe the properties of synchrotron‐generated X‐rays and the advantages that they provide for qualitative morphological research of millimetre‐sized bio...... hiện toàn bộ
Electron microscopy of critical point dried whole cultured cells
Journal of Microscopy - Tập 104 Số 2 - Trang 107-120 - 1975
Ian K. Buckley, Keith R. Porter
SUMMARYTo determine the overall fine structure of whole, unsectioned cells, cells from rat embryos were cultured on Formvar, glutaraldehyde/osmium‐fixed, transferred to grids, dehydrated, critical point dried, then examined by transmission electron microscopy at either 80 or 1000 kV. In contrast to air‐dried material, critical point dried cells revealed each compon...... hiện toàn bộ
A pixel selection rule based on the number of different‐phase neighbours for the simulated annealing reconstruction of sandstone microstructure
Journal of Microscopy - Tập 234 Số 3 - Trang 262-268 - 2009
Ting Tang, Qizhi Teng, Xiaohai He, Dan Luo
SummarySandstone reservoir is one of the main types of oil and gas reservoirs in China. It has porous microstructure, which directly affects the transport properties of a sandstone. Hence, the study of porous microstructure is important to the exploration and exploitation of oil and gas. Three‐dimensional microstructure of a sandstone can be reconstructed using the...... hiện toàn bộ
3D imaging of cement‐based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioning
Journal of Microscopy - Tập 258 Số 2 - Trang 151-169 - 2015
Marcus Yio, M.J. Mac, H.S. Wong, N.R. Buenfeld
SummaryIn this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z‐stacks, which are then aligned and stitched based on phase correlation. The method can...... hiện toàn bộ
The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopy
Journal of Microscopy - Tập 112 Số 1 - Trang 103-113 - 1978
T. Gulik‐Krzywicki, M. Joseph Costello
SUMMARYTwo methods of freezing samples for freeze‐fracture electron microscopy have been compared using X‐ray diffraction and freeze‐fracture results from lipid‐water model systems. Perturbations of the molecular organization of hydrocarbon chains and the extent of ice crystal formation have been evaluated for lamellar phases of egg lecithin containing 16% water an...... hiện toàn bộ
Cryo-SEM method for the observation of entrapped bubbles and degree of water filling in large wet powder compacts
Journal of Microscopy - Tập 242 Số 2 - Trang 189-196 - 2011
Johanne Mouzon, Iftekhar Uddin Bhuiyan, S.P.E. Forsmo, Jonas Hedlund
A physical phantom for the calibration of three‐dimensional X‐ray microtomography examination
Journal of Microscopy - Tập 222 Số 2 - Trang 124-134 - 2006
Egon Perilli, Fabio Baruffaldi, Maria Cristina Bisi, Luca Cristofolini, Angelo Cappello
SummaryX‐ray microtomography is rapidly gaining importance as a non‐destructive investigation technique, especially in the three‐dimensional examination of trabecular bone. Appropriate quantitative three‐dimensional parameters describing the investigated structure were introduced, such as the model‐independent thickness and the structure model index. The first para...... hiện toàn bộ
Polylysine as an adhesive for the attachment of nanoplankton to substrates for electron microscopy
Journal of Microscopy - Tập 131 Số 1 - Trang 127-129 - 1983
Harvey J. Marchant, D. Paul Thomas
SUMMARYNanoplankton can be readily attached to polylysine‐coated substrates for examination by transmission and scanning electron microscopy. This simple technique gives high yields of organisms which are consistently better preserved and less obscured by detritus than samples dried directly on to substrates.
Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopy
Journal of Microscopy - Tập 200 Số 1 - Trang 14-25 - 2000
Paul W. Wiseman, Jeffrey A. Squier, Mark H. Ellisman, Kent R. Wilson
We introduce two‐photon image correlation spectroscopy (ICS) using a video rate capable multiphoton microscope. We demonstrate how video rate two‐photon microscopic imaging and image correlation analysis may be combined to measure molecular transport properties over ranges typical of biomolecules in membrane environments. Using two‐photon ICS, we measured diffusion coefficients as large as...... hiện toàn bộ
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