Journal of Microscopy
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3D imaging of cement‐based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioningSummary In this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z‐stacks, which are then aligned and stitched based on phase correlation. The method can... ...
Journal of Microscopy - Tập 258 Số 2 - Trang 151-169 - 2015
The use of low temperature X‐ray diffraction to evaluate freezing methods used in freeze‐fracture electron microscopySUMMARY Two methods of freezing samples for freeze‐fracture electron microscopy have been compared using X‐ray diffraction and freeze‐fracture results from lipid‐water model systems. Perturbations of the molecular organization of hydrocarbon chains and the extent of ice crystal formation have been evaluated for lamellar phases of egg lecithin containing 16% water an... ...
Journal of Microscopy - Tập 112 Số 1 - Trang 103-113 - 1978
Cryo-SEM method for the observation of entrapped bubbles and degree of water filling in large wet powder compacts
Journal of Microscopy - Tập 242 Số 2 - Trang 189-196 - 2011
A physical phantom for the calibration of three‐dimensional X‐ray microtomography examinationSummary X‐ray microtomography is rapidly gaining importance as a non‐destructive investigation technique, especially in the three‐dimensional examination of trabecular bone. Appropriate quantitative three‐dimensional parameters describing the investigated structure were introduced, such as the model‐independent thickness and the structure model index. The first para... ...
Journal of Microscopy - Tập 222 Số 2 - Trang 124-134 - 2006
Polylysine as an adhesive for the attachment of nanoplankton to substrates for electron microscopySUMMARY Nanoplankton can be readily attached to polylysine‐coated substrates for examination by transmission and scanning electron microscopy. This simple technique gives high yields of organisms which are consistently better preserved and less obscured by detritus than samples dried directly on to substrates.
Journal of Microscopy - Tập 131 Số 1 - Trang 127-129 - 1983
Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopyWe introduce two‐photon image correlation spectroscopy (ICS) using a video rate capable multiphoton microscope. We demonstrate how video rate two‐photon microscopic imaging and image correlation analysis may be combined to measure molecular transport properties over ranges typical of biomolecules in membrane environments. Using two‐photon ICS, we measured diffusion coefficients as large as... ...
Journal of Microscopy - Tập 200 Số 1 - Trang 14-25 - 2000
Optical patterning in azobenzene polymer filmsSummary Thin azobenzene polymer films show a very unusual property, namely optically induced material transport. The underlying physics for this phenomenon has not yet been thoroughly explained. Nevertheless, this effect enables one to inscribe different patterns onto film surfaces, including one‐ and two‐dimensional periodic structures. Typical sizes of such struct... ...
Journal of Microscopy - Tập 219 Số 3 - Trang 109-114 - 2005
An investigation into microstructure and particle distribution of Ni–P/diamond composite thin filmsThe present paper deals with a theoretical and experimental study of the co‐deposition of spheroidal particles in a metal matrix deposited by autocatalytic chemical deposition (ACD). The 3‐D particle size distribution — as measured by image analysis of SEM micrographs of metallographic cross‐sections — is related to the size distribution of the starting powder — as measured by photon autoc... ...
Journal of Microscopy - Tập 185 Số 2 - Trang 283-291 - 1997
Application of back‐scattered electron imaging to the study of the lichen‐rock interfaceSummary In the study of the lichen‐rock interface, light microscopy, scanning electron microscopy (SEM) in secondary emission mode and transmission electron microscopy are the most commonly used techniques. As these methods have some limitations, there is a need to explore other techniques for observation of the lichen‐substrate interface. One of the most promising ... ...
Journal of Microscopy - Tập 175 Số 1 - Trang 54-59 - 1994
A new preparation method to study fresh plant structures with X‐ray computed tomographySummary Since the development of X‐ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good‐quality images. Therefore, we present a new preparation method for fresh material which incl... ...
Journal of Microscopy - Tập 233 Số 1 - Trang 1-4 - 2009
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