A new preparation method to study fresh plant structures with X‐ray computed tomography

Journal of Microscopy - Tập 233 Số 1 - Trang 1-4 - 2009
Olivier Leroux1, Manfred Schlosser2, E. Bellefroid1, Myriam Claeys2, Marjolein Couvreur2, Gaëtan Borgonie2, Luc Van Hoorebeke3, Bert Masschaele3, Ronald Viane1
1Research Group Pteridology, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium
2Nematology Unit, Department of Biology, Ghent University, K. L. Ledeganckstraat 35, B‐9000 Ghent, Belgium
3Centre for X-ray tomography, Institute for Nuclear Sciences, Ghent University, Proeftuinstraat 86, B-9000 Ghent, Belgium

Tóm tắt

SummarySince the development of X‐ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good‐quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.

Từ khóa


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Journal of Microscopy

Tập 233 Số 1

1-4

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