Journal of Eukaryotic Microbiology

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A Phylogenetic Lineage of Closely Related Trypanosomes (Trypanosomatidae, Kinetoplastida) of Anurans and Sand Flies (Psychodidae, Diptera) Sharing the Same Ecotopes in Brazilian Amazonia1
Journal of Eukaryotic Microbiology - Tập 55 Số 5 - Trang 427-435 - 2008
Robson C. Ferreira, A. A. de Souza, Rui A. de Freitas, Marta Campaner, CARMEM S. A. TAKATA, Toby V. Barrett, Jeffrey Jon Shaw, Marta M. G. Teixeira
ABSTRACT. Analysis of the phylogenetic relationships among trypanosomes from vertebrates and invertebrates disclosed a new lineage of trypanosomes circulating among anurans and sand flies that share the same ecotopes in Brazilian Amazonia. This assemblage of closely related trypanosomes was determined by comparing whole SSU rDNA sequences of anuran trypanosomes from the Brazilian biomes of Amazoni... hiện toàn bộ
Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens
Journal of Eukaryotic Microbiology - Tập 49 Số 6 - Trang 433-440 - 2002
Una Ryan, Abbie Fall, Lucy Ward, Nawal Hijjawi, Irshad M. Sulaiman, Ronald D. Payer, Richard C. Thompson, Merle S. Olson, Altaf A. Lal, Lihua Xiao
ABSTRACT. The structure and infectivity of the oocysts of a new species of Cryptosporidium from the feces of humans are described. Oocysts are structurally indistinguishable from those of Cryptosporidium parvum. Oocysts of the new species are passed fully sporulated, lack sporocysts, and measure 4.4–5.4 μm (mean = 4.86) × 4.4–5.9 μm (mean = 5.2μm) with a length to width ratio 1.0–1.09 (mean 1.07) ... hiện toàn bộ
Cryptosporidium Species and Genotypes in HIV‐Positive Patients in Lima, Peru
Journal of Eukaryotic Microbiology - Tập 50 Số s1 - Trang 531-533 - 2003
Vitaliano Cama, Caryn Bern, Irshad M. Sulaiman, Robert H. Gilman, Eduardo Ticona, Aldo Vivar, Vivian Kawai, Daniel Vargas, Ling Zhou, Lihua Xiao
ABSTRACT: Cryptosporidium parasites from a cross‐sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to deteimine the diversity of Cryptosporidium spp. in HIV‐positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of... hiện toàn bộ
Phylogenetic Distance of Thelohania butleriJohnston, Vernick, and Sprague, 1978 (Microsporidia; Thelohaniidae), a Parasite of the Smooth Pink Shrimp Pandalus jordani, from its Congeners Suggests Need for Major Revision of the Genus Thelohania Henneguy, 1892
Journal of Eukaryotic Microbiology - Tập 53 Số 6 - Trang 445-455 - 2006
Amanda M. V. Brown, Martin L. Adamson
ABSTRACT. Thelohania butleri, a microsporidian that causes mortality and commercial losses in the smooth pink shrimp Pandalus jordani, is of taxonomic interest as a species resembling the poorly studied type species, Thelohania giardi, of the large, polyphyletic genus Thelohania. We examined the ultrastructure of T. butleri to confirm its identity and reconstructed phylogenies using ribosomal DNA ... hiện toàn bộ
Relationships of Microsporidian Genera, with Emphasis on the Polysporous Genera, Revealed by Sequences of the Largest Subunit of RNA Polymerase II (RPB1)
Journal of Eukaryotic Microbiology - Tập 48 Số 1 - Trang 111-117 - 2001
Sarah A. Cheney, Nathalie J. Lafranchi-Tristem, Dorothée Bourges, Elizabeth U. Canning
ABSTRACT. Molecular data have proved useful as an alternative to morphological data in showing the relationships of genera within the phylum Microsporidia, but until now have been available only for ribosomal genes. In previous studies protein‐coding genes of microsporidia have been used only to assess their position in the evolution of eukaryotes. For the first time we report on the use of a prot... hiện toàn bộ
In Vitro Propagation of the Protozoan Perkinsus Marinus, A Pathogen of the Eastern Oyster, Crassostrea Virginica
Journal of Eukaryotic Microbiology - Tập 40 Số 3 - Trang 304-310 - 1993
Jérôme F. La Peyre, Mohamed Faisal, Eugene M. Burreson
ABSTRACT. Perkinsus marinus, a pathogen of eastern oysters (Crassostrea virginica), has been successfully propagated in vitro. Cultures of the parasite were initiated from heart fragments of an infected oyster. the cultured protozoan (designated Parkinsus‐1) was similar in morphology at both the light and transmission electron microscopy levels to histozoic stages of P. marinus in naturally infect... hiện toàn bộ
Multiple Polymorphic Sites at the ITS and ATAN Loci in Cultured Isolates of Perkinsus marinus
Journal of Eukaryotic Microbiology - Tập 51 Số 3 - Trang 312-320 - 2004
Gwynne D. Brown, Karen Hudson, Kimberly S. Reece
ABSTRACT. Sequence analysis of genomic DNA from the protozoan parasite Perkinsus marinus at two loci revealed genetic polymorphisms within and among different cultured isolates. Genomic DNA from 12 Perkinsus marinus isolates was amplified at the internal transcribed spacer region and at an anonymous locus previously identified to contain polymorphisms by restriction fragment length polymorphism an... hiện toàn bộ
In Vitro Propagation of Two Perkinsus spp. Parasites from Japanese Manila Clams Venerupis philippinarum and Description of Perkinsus honshuensis n. sp.
Journal of Eukaryotic Microbiology - Tập 53 Số 5 - Trang 316-326 - 2006
Christopher F. Dungan, Kimberly S. Reece
ABSTRACT. Perkinsus species are destructive parasites of commercial Manila clams, Venerupis philippinarum, in Japan, Korea, and Spain. However, in vitro parasite cultures from this important host clam are not available. Tissues of Manila clams collected during April 2002 in Gokasho Bay, Japan harbored Perkinsus sp. parasites at a 97% prevalence (28/29) of moderate‐ and high‐intensity infections. P... hiện toàn bộ
Molecular, Morphological, and Experimental Evidence Support the Synonymy of Perkinsus chesapeaki and Perkinsus andrewsi
Journal of Eukaryotic Microbiology - Tập 52 Số 3 - Trang 258-270 - 2005
Eugene M. Burreson, Kimberly S. Reece, Christopher F. Dungan
Abstract. Diverse analytical and experimental results confirm that two protistan parasites, Perkinsus chesapeaki and Perkinsus andrewsi, described separately as parasites of Mya arenaria and Macoma balthica clams sympatric in Chesapeake Bay, USA, represent a single species. Ribosomal RNA (rRNA) internal transcribed spacer (ITS) regions, rRNA large subunit (LSU) gene, and actin gene sequences were ... hiện toàn bộ
Description of Perkinsus andrewsi n. sp. Isolated from the Baltic Clam (Macoma balthica) by Characterization of the Ribosomal RNA Locus, and Development of a Species‐Specific PCR‐Based Diagnostic Assay
Journal of Eukaryotic Microbiology - Tập 48 Số 1 - Trang 52-61 - 2001
Cathleen A. Coss, José A. Fernández Robledo, Gregory M. Ruiz, Gerardo R. Vasta
ABSTRACT. A Perkinsus species was isolated from the baltic clam Macoma balthica and an in vitro culture established under conditions described for P. marinus. As reported previously, morphological features remarkable enough to clearly indicate that this isolate is a distinct Perkinsus species were lacking. In this study, regions of the rRNA locus (NTS, 18S. ITS1, 5.8S. and 1TS2) of this isolate we... hiện toàn bộ
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