Incorporation of Label from Acetate and Laurate into the Mannan of Leishmania donovani via the Glyoxylate Cycle

Journal of Eukaryotic Microbiology - Tập 40 Số 6 - Trang 730-732 - 1993
FRANK P. KEEGAN1, J.J. Blum2
1Department of Biology, Guilford College, Greensboro, North Carolina 27410
2Division of Physiology, Department of Cell Biology and Cellular Biophysics, Duke University Medical Center, Durham, North Carolina 27710

Tóm tắt

ABSTRACT Leishmania donovani promastigotes in late‐stationary phase incorporated label from [2‐14C]acetate and [1‐14C]laurate into the mannose residues of mannan, thus confirming the presence of a functional glyoxylate bypass in these parasitic protozoa. Isolated, washed calls also incorporated label from [2‐14C]acetate and [1‐14C]laurate into mannan during a 1‐hr incubation in buffer. Glucose had no effect on label incorporation into mannan, but glutamate caused over a four‐fold increase in incorporation from [2‐14C]acetate and a 2.4‐fold increase from [1‐14C]laurate. Staurosporine, a protein kinase inhibitor that inhibits glutamate and alanine oxidation, did not inhibit label incorporation from [2‐14C]acetate into mannan. Hyperosmolality caused about a 33% inhibition of label incorporation into mannan. These results show the glyoxylate cycle and/or the subsequent biosynthetic pathway from fructose‐6‐phosphate to mannan are subject to regulation.

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Thông tin xuất bản

Journal of Eukaryotic Microbiology

Tập 40 Số 6

730-732

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