Characterization of Two Species of Trypanosomatidae from the Honey Bee Apis mellifera: Crithidia mellificae Langridge and McGhee, and Lotmaria passim n. gen., n. sp.

Journal of Eukaryotic Microbiology - Tập 62 Số 5 - Trang 567-583 - 2015
Ryan S. Schwarz1, Gary R. Bauchan2, Charles Murphy2, Jorgen Ravoet3, Dirk C. de Graaf3, Jay D. Evans1
1Bee Research Laboratory, Beltsville Agricultural Research Center - East, U.S. Department of Agriculture, Bldg 306, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
2Electron and Confocal Microscopy Unit; Beltsville Agricultural Research Center - West; U.S. Department of Agriculture; Bldg 012 10300 Baltimore Ave. Beltsville MD 20705 USA
3Laboratory of Zoophysiology; Faculty of Science; Ghent University; Ghent Belgium

Tóm tắt

AbstractTrypanosomatids are increasingly recognized as prevalent in European honey bees (Apis mellifera) and by default are attributed to one recognized species, Crithidia mellificae Langridge and McGhee, 1967. We provide reference genetic and ultrastructural data for type isolates of C. mellificae (ATCC 30254 and 30862) in comparison with two recent isolates from A. mellifera (BRL and SF). Phylogenetics unambiguously identify strains BRL/SF as a novel taxonomic unit distinct from C. mellificae strains 30254/30862 and assign all four strains as lineages of a novel clade within the subfamily Leishmaniinae. In vivo analyses show strains BRL/SF preferably colonize the hindgut, lining the lumen as adherent spheroids in a manner identical to previous descriptions from C. mellificae. Microscopy images show motile forms of C. mellificae are distinct from strains BRL/SF. We propose the binomial Lotmaria passim n. gen., n. sp. for this previously undescribed taxon. Analyses of new and previously accessioned genetic data show C. mellificae is still extant in bee populations, however, L. passim n. gen., n. sp. is currently the predominant trypanosomatid in A. mellifera globally. Our findings require that previous reports of C. mellificae be reconsidered and that subsequent trypanosomatid species designations from Hymenoptera provide genetic support.

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Journal of Eukaryotic Microbiology

Tập 62 Số 5

567-583

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