European Journal of Oral Sciences
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Tần suất và các yếu tố liên quan của đau răng: một nghiên cứu theo dòng đời từ lúc sinh đến 12 tuổi Nghiên cứu này ước tính tỷ lệ đau răng trọn đời ở độ tuổi 6 và 12 tuổi, tỷ lệ đau răng trong tháng vừa qua và sự liên quan của chúng với các yếu tố xã hội, hành vi và lâm sàng trong suốt cuộc đời của 339 trẻ 12 tuổi từ một đoàn hệ sinh ở Pelotas, Brazil. Các biến số khám phá được thu thập từ nghiên cứu chu sinh và trong nhiều nghiên cứu theo dõi. Các tỷ lệ thịnh hành được tính toán bằng hồi quy Poisson, theo một mô hình khái niệm phân cấp. Tỷ lệ đau răng trọn đời ở độ tuổi 6 và 12 tuổi lần lượt là 39% [khoảng tin cậy 95% (CI) = (34;45)] và 63% [95% CI = (58;69)]. Đau răng trong tháng qua được báo cáo bởi 11% [95% CI = (8;15)]. Trẻ không sống cùng cha ruột khi sinh và trẻ có chỉ số dmf-t cao hơn báo cáo tỷ lệ đau răng trọn đời cao hơn ở độ tuổi 6 tuổi. Trẻ trải qua nghèo đói từ 0 đến 4 năm tuổi, có chỉ số dmf-t và DMF-T cao hơn có tỷ lệ đau răng trọn đời lớn hơn ở độ tuổi 12 tuổi. Đau răng trong tháng qua có khả năng được báo cáo nhiều hơn bởi các bé gái và trẻ không sống với cha ruột khi sinh. Chiến lược phòng ngừa nên được thực hiện ở giai đoạn đầu của vòng đời, cần tính đến bối cảnh kinh tế-xã hội và gia đình nơi mà đau đớn thường xảy ra.
European Journal of Oral Sciences - Tập 116 Số 5 - Trang 458-466 - 2008
#đau răng #tần suất #yếu tố liên quan #nghiên cứu cuộc sống #chỉ số dmf-t #chỉ số DMF-T #kinh tế-xã hội #bối cảnh gia đình #Brazil #trẻ em
<i>In situ</i> identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence <i>in situ</i> hybridization Confocal laser scanning microscopy (CLSM) has been employed as a method for studying intact natural biofilm. When combined with fluorescence in situ hybridization (FISH) it is possible to analyze spatial relationships and changes of specific members of microbial populations over time. The aim of this study was to perform a systematic description of the pattern of initial dental biofilm formation by applying 16S rRNA‐targeted oligonucleotide probes to the identification of streptococci and other bacteria, and to evaluate the usefulness of the combination of CLSM and FISH for structural studies of bacterial populations in dental biofilm. Biofilms were collected on standardized glass slabs mounted in intra‐oral appliances and worn by 10 individuals for 6, 12, 24 or 48 h. After intra‐oral exposure the biofilms were labelled with probes against either streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. The current approach of using FISH techniques enabled differentiation of streptococci from other bacteria and determination of their spatio‐temporal organization. The presence of chimney‐like multilayered microcolonies with different microbial compositions demonstrated by this methodology provided information supplementary to our previous knowledge obtained by classical electron microscopic methods and increased our understanding of the structure of developing biofilms.
European Journal of Oral Sciences - Tập 115 Số 6 - Trang 459-467 - 2007
Bioactive effects of a calcium/sodium phosphosilicate on the resin–dentine interface: a microtensile bond strength, scanning electron microscopy, and confocal microscopy study This study evaluated, through microtensile bond strength (μTBS ) testing, the bioactive effects of a calcium/sodium phosphosilicate (BAG ) at the resin–dentine interface after 6 months of storage in phosphate buffer solution (PBS). Confocal laser scanning microscopy (CLSM ) and scanning electron microscopy (SEM ) were also performed. Three bonding protocols were evaluated: (i) RES ‐C tr (no use of BAG ), (ii) BAG containing adhesive (BAG ‐AD ), and (iii) BAG /H
3
PO
4 before adhesive (BAG ‐PR ). The dentin‐bonded specimens were prepared for μTBS testing, which was carried out after 24 h or 6 months of storage in PBS . Scanning electron microscopy ultramorphology analysis was performed after debonding. Confocal laser scanning microscopy was used to evaluate the morphological and nanoleakage changes induced by PBS storage. High μTBS values were achieved in all groups after 24 h of storage in PBS. Subsequent to 6 months of storage in PBS the specimens created using the BAG‐AD bonding approach still showed no significant reduction in μTBS. Moreover, specimens created using the BAG‐AD or the BAG‐PR approach showed an evident reduction of nanoleakage after prolonged storage in PBS. The use of BAG ‐containing adhesive may enhance the durability of the resin–dentine bonds through therapeutic/protective effects associated with mineral deposition within the bonding interface and a possible interference with collagenolytic enzyme activity (matrix metalloproteinases) responsible for the degradation of the hybrid layer.
European Journal of Oral Sciences - Tập 120 Số 4 - Trang 353-362 - 2012
Irradiation effects on microhardness of fluoridated and non‐fluoridated bovine dentin The objective of this study was to evaluate the effects of irradiation on microhardness of dentin. Dentin blocks from the cervical region of bovine incisors were treated as follows: (1) no irradiation; (2) irradiation of specimens up to 60 Gy (2 Gy/day, 5 days/week); (3) no irradiation, but fluoridation of specimens for 5 min/d; (4) irradiation of specimens and daily fluoridation. Knoop hardness number (KHN) of the control specimens was 62.63 ±14.75 (mean ± SD). This was significantly different from the irradiated dentin samples (8.74 ± 2.59 KHN). Hardness of the fluoridated dentin specimens was 11.19 ± 1.95 KHN in the non‐irradiated group and 10.03±2.76 KHN in the irradiated groups, respectively. Within the limitations of an in vitro study, it is concluded that dentin is severely affected by irradiation. This could be an explanation for the frequently observed side‐effects of irradiation like loss of enamel, gap formation at the amelodentinal junction, and caries of the cervical region. Fluoridation with acidic gels decreases microhardness of dentin surface, and does not prevent softening due to radiation, when saliva is absent.
European Journal of Oral Sciences - Tập 105 Số 5P1 - Trang 444-447 - 1997
Effect of <scp>pH</scp> on dentin protease inactivation by carbodiimide A water‐soluble crosslinking agent, 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide (EDC ), has been used as a pretreatment of acid‐etched dentin to inactivate matrix‐bound endogenous dentin proteases. The aim of this study was to evaluate the effect of pH on the inactivation capacity of EDC . Demineralized dentin beams (1 × 2 × 6 mm) were divided into six groups (n = 8 per group). Then, EDC (0.3 M) was solubilized in distilled water with pH of 2, 4, 6, 7, 9, or 11. Control EDC was solubilized in 0.1 M 2‐(N ‐morpholino) ethanesulfonic acid (MES ) buffer and its pH was adjusted to 6. The dentin beams were pretreated for 1 min with EDC at each pH or with EDC in MES buffer at pH 6.0 and then incubated in 1 ml of simulated body fluid (pH 7.2) for 1, 3, 7, or 14 d. Untreated beams served as controls. At each study time‐point, the dry mass of dentin beams was assessed and the incubation media were analyzed for carboxyterminal telopeptide of type‐I collagen (ICTP ) and C‐terminal telopeptide of type I collagen (CTX ) using specific ELISA s. Data were subjected to repeat‐measures anova . The results of the study indicated that specimens pretreated with EDC in MES buffer showed the lowest collagen degradation in terms of mass loss and release of telopeptides, while specimens pretreated in alkaline media showed the highest collagen degradation. This study indicates that the pH of the EDC solution plays an important role in the stability of dentin protease inactivation.
European Journal of Oral Sciences - Tập 125 Số 4 - Trang 288-293 - 2017
Influence of chlorhexidine digluconate concentration and application time on resin–dentin bond strength durability Although it is known that chlorhexidine application may preserve resin–dentin bonds from degradation, the lowest optimal concentration and application time have yet to be established. This study evaluated the effects of different concentrations of chlorhexidine digluconate and different application times on the preservation of resin–dentin bonds formed using two etch‐and‐rinse adhesives. In experiment 1, after acid etching, the occlusal demineralized dentin was rewetted either with water or with 0.002, 0.02, 0.2, 2, or 4% chlorhexidine for 60 s. In experiment 2, the surfaces were rewetted with water, or with 0.002% or 2% chlorhexidine for 15 or 60 s. After this, both adhesives and composite resin were applied and light‐cured. Bonded sticks (0.8 mm2 ) were tested under tension (0.5 mm min−1 ) immediately or after 6 months of storage in water. Two bonded sticks from each tooth were immersed in silver nitrate and analyzed quantitatively using scanning electron microscopy. Reductions in microtensile bond strengths and higher silver nitrate uptake were observed for both adhesives when the rewetting procedure was performed with water. Stable bonds were maintained for up to 6 months under all chlorhexidine conditions tested, irrespective of the chlorhexidine concentration and application time. The use of 0.002% chlorhexidine for 15 s seems to be sufficient to preserve resin–dentin interfaces over a 6‐month period.
European Journal of Oral Sciences - Tập 117 Số 5 - Trang 587-596 - 2009
Influence of cavity design on the biomechanics of direct composite resin restorations in Class IV preparations Xu H, Jiang Z, Xiao X, Fu J, Su Q. Influence of cavity design on the biomechanics of direct composite resin restorations in Class IV preparations.
Eur J Oral Sci 2012; 120: 161–167. © 2012 Eur J Oral Sci This study evaluated the effect of cavity design on stress distribution and fracture resistance of direct composite resin restorations in Class IV preparations. A finite element analysis (FEA) model of the maxillary central incisor with a Class IV cavity was established. Five model variations were studied: (i) a 1‐mm bevel (ii) a 2‐mm bevel, (iii) a plain chamfer, (iv) a stair‐step chamfer, and (v) butt joints (a control configuration). All FEA variations modeled a tooth restored with composite resin loaded under 100 N at an angle of 45° to the longitudinal axis. The interfacial von Mises stress was evaluated. The FEA was complemented with an in vitro assessment. Fracture resistance of direct composite resin restorations was tested with a universal testing machine and fracture patterns were observed. Finite element analysis showed that stress in chamfer and stair‐step chamfer models was more homogenously distributed, while stress in bevel models was relatively concentrated at lingual regions. Fracture resistance of a 1‐mm bevel preparation was lower than for the 2‐mm bevel, plain chamfer, and stair‐step chamfer preparations, but was higher than for butt joints. The stair‐step chamfer group presented the most favorable failure pattern. Considering biomechanics and esthetics, the present study indicates that the stair‐step chamfer and 2‐mm bevel should be recommended for clinical restoration.
European Journal of Oral Sciences - Tập 120 Số 2 - Trang 161-167 - 2012
Interleukin‐17 plays a role in exacerbation of inflammation within chronic periapical lesions Interleukin (IL)‐17 plays an important role in inflammation and certain autoimmune diseases. However, its role in the pathogenesis of chronic dental periapical lesions has not been studied. Periapical lesion mononuclear cells (PL‐MNC) were isolated from inflammatory cells and phenotypically analyzed by immunocytochemistry. The cells were cultured in vitro and IL‐17 and IL‐8 were measured in the culture supernatants. Controls were peripheral blood (PB) MNC. The level of IL‐17 and the proportion of neutrophils were significantly higher in symptomatic lesions. In addition, the production of IL‐17 was higher in culture supernatants of PL‐MNC isolated from lesions with a predominance of T cells, and the IL‐17 concentration correlated with the proportion of CD3+ and CD4+ cells. There was a positive correlation between the levels of IL‐17 and IL‐8 in the group of symptomatic lesions. The relationship between these cytokines was additionally confirmed on the basis of augmented production of IL‐8 by both PL‐MNC and PB‐MNC treated with IL‐17. Our results suggest that IL‐17, by stimulating the production of IL‐8, may play a role in exacerbating inflammation within chronic periapical lesions.
European Journal of Oral Sciences - Tập 115 Số 4 - Trang 315-320 - 2007
Age‐dependent changes in insulin‐like immunoreactivity in rat submandibular salivary glands In recent years, a growing interest had arisen in hormonal factors in salivary glands. We have investigated the changes in the content of an insulin‐like immunoreactive (ILI) compound in the submandibular salivary glands of Sprague Dawley rats during physiological aging, in the range 15 days ‐27 months. The amount of ILI in the submandibular glands of young adult rats was found to be doubled in the post‐natal period until the age of puberty and was maintained in senescence. No significant correlation was found between age‐dependent variations in ILI levels of submandibular salivary glands and circulating insulin concentrations, further supporting previous indications that ILI is being synthesized in situ. It is possible that ILI could exert paracrine effects within the glands, as regards the development of other glandular structures during the first months of life, as well as the preservation of glandular function in senescent animals as well.
European Journal of Oral Sciences - Tập 104 Số 5-6 - Trang 630-633 - 1996
Susceptibility of <i>Streptococcus mutans</i> and <i>Streptococcus sobrinus</i> to antimicrobial agents after short‐term oral chlorhexidine treatments Effects of three different types of short‐term applications (1–3 limes during 1 week) of chlorhexidine (1 or 40%) on the susceptibility of 863 clinical isolates of Streptococcus mutans and 53 isolates of Streptococcus sobrinus from 58 subjects were studied. Chlorhexidine‐resistant isolates were not found either before or after the treatment. The minimum inhibitory concentrations (MICs) to chlorhexidine of all isolates of S. mutans were ≤1 μg/ml, and of S. sobrinus ≤2 μg/ ml. S. mutans and S. sobrinus were also susceptible to ampicillin, penicillin, cefuroxime, and tetracycline. In conclusion, different short‐term chlorhexidine regimens do not induce resistance in S. mutans or S. sobrinus and, furthermore, these species have so far retained their susceptibility to common antibiotics.
European Journal of Oral Sciences - Tập 103 Số 1 - Trang 32-35 - 1995
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