Electrophoresis
SCOPUS (1980-2023)SCIE-ISI
0173-0835
1522-2683
Đức
Cơ quản chủ quản: WILEY , Wiley-VCH Verlag
Lĩnh vực:
BiochemistryClinical BiochemistryAnalytical Chemistry
Các bài báo tiêu biểu
SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modelingAbstract Comparative protein modeling is increasingly gaining interest since it is of great assistance during the rational design of mutagenesis experiments. The availability of this method, and the resulting models, has however been restricted by the availability of expensive computer hardware and software. To overcome these limitations, we have developed an enviro... ... hiện toàn bộ
Tập 18 Số 15 - Trang 2714-2723 - 1997
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gelsAbstract A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development. This procedure provides high sensitivity for proteins, RNA and DNA in the nanogram range o... ... hiện toàn bộ
Tập 8 Số 2 - Trang 93-99 - 1987
Difference gel electrophoresis. A single gel method for detecting changes in protein extractsAbstract We describe a modification of two‐dimensional (2‐D) polyacrylamide gel electrophoresis that requires only a single gel to reproducibly detect differences between two protein samples. This was accomplished by fluorescently tagging the two samples with two different dyes, running them on the same 2‐D gel, post‐run fluorescence imaging of the gel into two imag... ... hiện toàn bộ
Tập 18 Số 11 - Trang 2071-2077 - 1997
Automated comparative protein structure modeling with SWISS‐MODEL and Swiss‐PdbViewer: A historical perspectiveAbstract SWISS‐MODEL pioneered the field of automated modeling as the first protein modeling service on the Internet. In combination with the visualization tool Swiss‐PdbViewer, the Internet‐based Workspace and the SWISS‐MODEL Repository, it provides a fully integrated sequence to structure analysis and modeling platform. This computational environment is made freel... ... hiện toàn bộ
Tập 30 Số S1 - 2009
Blue silver: A very sensitive colloidal Coomassie G‐250 staining for proteome analysisAbstract A modified Neuhoff's colloidal Coomassie Blue G‐250 stain is reported, dubbed “blue silver” on account of its considerably higher sensitivity, approaching the one of conventional silver staining. The main modifications, as compared to Neuhoff's protocol, were: a 20% increment in dye concentration (from 0.1% up to 0.12%) and a much higher level of phosphoric... ... hiện toàn bộ
Tập 25 Số 9 - Trang 1327-1333 - 2004
Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteinsAbstract The level of genetic variation revealed by two‐dimensional electrophoresis of proteins from seedlings of two wheat lines strongly depends on the technical procedures. Improvements in extraction and electrophoresis procedures relative to earlier experiments on the same material led to a significant increase in the genetic variation revealed: 15.2 % instead o... ... hiện toàn bộ
Tập 7 Số 1 - Trang 52-54 - 1986
Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genomeAbstract The two‐dimensional electrophoresis (2‐DE) technique developed by Klose in 1975 (Humangenetik 1975, 26 , 211–234), independently of the technique developed by O'Farrell (J. Biol. Chem . 1975, 250 , 4007–4021), has been revised in our laboratory and an up... ... hiện toàn bộ
Tập 16 Số 1 - Trang 1034-1059 - 1995
Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gelsAbstract A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detecti... ... hiện toàn bộ
Tập 9 Số 1 - Trang 28-32 - 1988
Rapid prototyping of paper‐based microfluidics with wax for low‐cost, portable bioassayAbstract Here we present a simple and low‐cost production method to generate paper‐based microfluidic devices with wax for portable bioassay. The wax patterning method we introduced here included three different ways: (i) painting with a wax pen, (ii) printing with an inkjet printer followed by painting with a wax pen, (iii) printing by a wax printer directly. The w... ... hiện toàn bộ
Tập 30 Số 9 - Trang 1497-1500 - 2009
Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresisAbstract We describe the extraction and enrichment of membrane proteins for separation by two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) after differential solubilization of an Escherichia coli cell lysate. In a simple three‐step sequential solubilization protocol applicable for whole cell lysates, membrane proteins are part... ... hiện toàn bộ
Tập 19 Số 5 - Trang 837-844 - 1998