Electrophoresis
SCOPUS (1980-2025)SCIE-ISI
0173-0835
1522-2683
Đức
Cơ quản chủ quản: WILEY , Wiley-VCH Verlag
Lĩnh vực:
BiochemistryClinical BiochemistryAnalytical Chemistry
Các bài báo tiêu biểu
SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modeling
Tập 18 Số 15 - Trang 2714-2723 - 1997
AbstractComparative protein modeling is increasingly gaining interest since it
is of great assistance during the rational design of mutagenesis experiments.
The availability of this method, and the resulting models, has however been
restricted by the availability of expensive computer hardware and software. To
overcome these limitations, we have developed an environment for comparative
protein mod...
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
Tập 8 Số 2 - Trang 93-99 - 1987
AbstractA new modification of silver staining is presented which utilizes two
chemical properties of thiosulfate: image enhancement by pretreatment of fixed
gels, and formation of soluble silver complexes which prevents unspecific
background staining during image development. This procedure provides high
sensitivity for proteins, RNA and DNA in the nanogram range on a colorless,
transparent backgr...
Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
Tập 18 Số 11 - Trang 2071-2077 - 1997
AbstractWe describe a modification of two‐dimensional (2‐D) polyacrylamide gel
electrophoresis that requires only a single gel to reproducibly detect
differences between two protein samples. This was accomplished by fluorescently
tagging the two samples with two different dyes, running them on the same 2‐D
gel, post‐run fluorescence imaging of the gel into two images, and superimposing
the images....
Automated comparative protein structure modeling with SWISS‐MODEL and Swiss‐PdbViewer: A historical perspective
Tập 30 Số S1 - 2009
AbstractSWISS‐MODEL pioneered the field of automated modeling as the first
protein modeling service on the Internet. In combination with the visualization
tool Swiss‐PdbViewer, the Internet‐based Workspace and the SWISS‐MODEL
Repository, it provides a fully integrated sequence to structure analysis and
modeling platform. This computational environment is made freely available to
the scientific com...
Blue silver: A very sensitive colloidal Coomassie G‐250 staining for proteome analysis
Tập 25 Số 9 - Trang 1327-1333 - 2004
AbstractA modified Neuhoff's colloidal Coomassie Blue G‐250 stain is reported,
dubbed “blue silver” on account of its considerably higher sensitivity,
approaching the one of conventional silver staining. The main modifications, as
compared to Neuhoff's protocol, were: a 20% increment in dye concentration (from
0.1% up to 0.12%) and a much higher level of phosphoric acid in the recipe (from
2% up t...
Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins
Tập 7 Số 1 - Trang 52-54 - 1986
AbstractThe level of genetic variation revealed by two‐dimensional
electrophoresis of proteins from seedlings of two wheat lines strongly depends
on the technical procedures. Improvements in extraction and electrophoresis
procedures relative to earlier experiments on the same material led to a
significant increase in the genetic variation revealed: 15.2 % instead of 6.7 %
of the spots were genetic...
Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome
Tập 16 Số 1 - Trang 1034-1059 - 1995
AbstractThe two‐dimensional electrophoresis (2‐DE) technique developed by Klose
in 1975 (Humangenetik 1975, 26, 211–234), independently of the technique
developed by O'Farrell (J. Biol. Chem. 1975, 250, 4007–4021), has been revised
in our laboratory and an updated protocol is presented. This protocol is the
result of our experience in using this method since its introduction. Many
modifications an...
Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels
Tập 9 Số 1 - Trang 28-32 - 1988
AbstractA new modification of silver staining of proteins in sodium dodecyl
sulfate polyacrylamide gels is adapted to automated staining in PhastSystem
Development Unit. The use of a reduction step, after fixation, with thiosulfate
in alcoholic sodium acetate buffer results in a considerable increase in
sensitivity without the need for a recycling step. The detection limit is
tenfold lower than in...
Rapid prototyping of paper‐based microfluidics with wax for low‐cost, portable bioassay
Tập 30 Số 9 - Trang 1497-1500 - 2009
AbstractHere we present a simple and low‐cost production method to generate
paper‐based microfluidic devices with wax for portable bioassay. The wax
patterning method we introduced here included three different ways: (i) painting
with a wax pen, (ii) printing with an inkjet printer followed by painting with a
wax pen, (iii) printing by a wax printer directly. The whole process was easy to
operate ...
Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis
Tập 19 Số 5 - Trang 837-844 - 1998
AbstractWe describe the extraction and enrichment of membrane proteins for
separation by two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE)
after differential solubilization of an Escherichia coli cell lysate. In a
simple three‐step sequential solubilization protocol applicable for whole cell
lysates, membrane proteins are partitioned from other cellular proteins by their
insolubility i...