Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins

Electrophoresis - Tập 7 Số 1 - Trang 52-54 - 1986
Catherine Damerval1, Dominique de Vienne1, Michel Zivy1, Hervé Thiellement1
1Laboratoire de Génétique des Systèmes Végétaux, G. S. Moulon Gif-sur-Yvette

Tóm tắt

AbstractThe level of genetic variation revealed by two‐dimensional electrophoresis of proteins from seedlings of two wheat lines strongly depends on the technical procedures. Improvements in extraction and electrophoresis procedures relative to earlier experiments on the same material led to a significant increase in the genetic variation revealed: 15.2 % instead of 6.7 % of the spots were genetically variable. The improved procedure is based on (i) precipipation of proteins from wheat seedlings with trichloroacetic acid and acetone, (ii) solubilization of the proteins with a solution containing urea, potassium carbonate and sodium dodecyl sulfate, (iii) isoelectric focusing in an optimized pH gradient, obtained with a mixture of carrier ampholytes (Pharmalyte and Servalyt), and (iv) running elecrophoresis in the second dimension on gels with increased surface.

Từ khóa


Tài liệu tham khảo

10.1016/S0021-9258(19)41496-8

10.1073/pnas.76.5.2381

10.1038/283855a0

10.1073/pnas.78.6.3784

Ohnishi S., 1982, Genetics, 100, 127, 10.1093/genetics/100.1.127

Avise J. C., 1983, Protein polymorphism: adaptive and taxonomic significance, 103

10.1038/284511a0

10.1038/288010a0

10.1073/pnas.79.10.3236

McLellan T., 1983, Genetics, 104, 381, 10.1093/genetics/104.2.381

10.1007/BF00281838

10.1007/BF00267887

10.1016/S0021-9258(18)36039-3

10.1016/0003-2697(80)90470-4

10.1104/pp.78.1.178

10.1073/pnas.80.16.5002

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Thông tin xuất bản

Electrophoresis

Tập 7 Số 1

52-54

Thông tin tác giả