Electrophoresis

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Two–dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: Discrimination of cultivars with different malting grades
Electrophoresis - Tập 13 Số 1 - Trang 192-203 - 1992
Angelika Görg, Wilhelm Postel, Max Baumer, Walter Weiss
AbstractThe suitability of high‐resolution two‐dimensional gel electrophoresis for barley cultivar discrimination and for classification with respect to their malting properties was studied. Seed proteins of 14 barley cultivars with different malting qualities were extracted with urea/dithiothreitol/Nonidet P‐40 buffer and subjected to two‐dimensional gel electroph...... hiện toàn bộ
Solubilization of cellular membrane proteins fromStreptococcus mutans for two-dimensional gel electrophoresis
Electrophoresis - Tập 26 Số 6 - Trang 1200-1205 - 2005
Kheir Zuobi-Hasona, Paula J. Crowley, Adnan Hasona, Arnold S. Bleiweis, L. Jeannine Brady
Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis
Electrophoresis - Tập 19 Số 5 - Trang 837-844 - 1998
Mark P. Molloy, Ben Herbert, Bradley J. Walsh, Margaret I. Tyler, Mathew Traini, Jean‐Charles Sanchez, Denis F. Hochstrasser, Keith L. Williams, Andrew A. Gooley
AbstractWe describe the extraction and enrichment of membrane proteins for separation by two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) after differential solubilization of an Escherichia coli cell lysate. In a simple three‐step sequential solubilization protocol applicable for whole cell lysates, membrane proteins are part...... hiện toàn bộ
Borate‐containing background electrolytes to improve CE separation in bare capillaries
Electrophoresis - Tập 41 Số 12 - Trang 1073-1080 - 2020
Vladislav Dolnı́k
AbstractThe paper describes how borate‐containing BGEs modify ζ‐potential and so EOF in bare fused silica capillaries. This surface modification can be used to suppress EOF and improve the separation performance of CZE including capillary sieving electrophoresis (CSE). Boric acid forms complexes with polysaccharides used as sieving matrices in CSE and other compoun...... hiện toàn bộ
Identification and characterization of heat shock protein 27 protein species in human myocardial two‐dimensional electrophoresis patterns
Electrophoresis - Tập 18 Số 15 - Trang 2823-2831 - 1997
Christian Scheler, Eva‐Christina Müller, Joachim Stahl, Ursula Müller‐Werdan, Johann Salnikow, Peter R. Jungblut
AbstractImmunostaining of heat shock protein 27 (Hsp27) protein species on two‐dimensional electrophoresis (2‐DE) gels with enhanced sensitivity yields 59 spots reacting with anti‐Hsp27 antibodies. Recombinant Hsp27 exists in 2‐DE as two major protein species which comigrate in the human myocardial pattern with Hsp27 spots C754 and D899 as defined in the heart high...... hiện toàn bộ
A compactly integrated laser‐induced fluorescence detector for microchip electrophoresis
Electrophoresis - Tập 25 Số 12 - Trang 1907-1915 - 2004
Hai‐Fang Li, Jin‐Ming Lin, Rongbing Su, Katsumi Uchiyama, Toshiyuki Hobo
AbstractA simple and easy‐to‐use integrated laser‐induced fluorescence detector for microchip electrophoresis was constructed and evaluated. The fluid channels and optical fiber channels in the glass microchip were fabricated using standard photolithographic techniques and wet chemical etching. A 473 nm diode‐pumped laser was used as the excitation source, and the ...... hiện toàn bộ
A rigid poly(dimethylsiloxane) sandwich electrophoresis microchip based on thin‐casting method
Electrophoresis - Tập 27 Số 14 - Trang 2917-2923 - 2006
Changchun Liu, Dafu Cui, Haoyuan Cai, Xing Chen, Zhaoxin Geng
AbstractWe present a novel concept of glass/poly(dimethylsiloxane) (PDMS)/glass sandwich microchip and developed a thin‐casting method for fabrication. Unlike the previously reported casting method for fabricating PDMS microchip, several drops of PDMS prepolymer were first added on the silanizing SU‐8 master, then another glass plate was placed over the prepolymer ...... hiện toàn bộ
Recent advances in capillary electrophoresis/electrospray/mass spectrometry
Electrophoresis - Tập 18 Số 12-13 - Trang 2255-2266 - 1997
J. Fred Banks
AbstractSuccessful on‐line interfacing of capillary electrophoresis (CE) with electrospray (ES) mass spectrometry (MS) has progressed substantially in recent years. Of particular note also is the development which has occurred in combining the more advanced capillary‐based electromigration separation techniques, such as capillary gel electrophoresis (CGE), capillar...... hiện toàn bộ
Development and validation of a capillary electrophoresis method for the determination of phenothiazines in human urine in the low nanogramper milliliter concentration range using field-amplified sample injection
Electrophoresis - Tập 26 Số 12 - Trang 2418-2429 - 2005
Francisco J. Lara, Ana M. García‐Campaña, Fermín Alés‐Barrero, Juan M. Bosque‐Sendra
Recent advances in the methodology, optimisation and application of MEEKC
Electrophoresis - Tập 30 Số 1 - Trang 65-82 - 2009
Richie Ryan, Sheila Donegan, Joe Power, Eamon McEvoy, Kevin D. Altria
AbstractMEEKC is an electrodriven separation technique. Oil‐in‐water microemulsions (MEs) and to a lesser extent water‐in‐oil MEs have been used in MEEKC as BGEs to achieve separation of a diverse range of solutes. The more common (oil‐in‐water) MEs are composed of nanometre‐sized droplets of oil suspended in an aqueous buffer. Interfacial tension between the oil a...... hiện toàn bộ
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