Electrophoresis
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Two–dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: Discrimination of cultivars with different malting grades
Electrophoresis - Tập 13 Số 1 - Trang 192-203 - 1992
AbstractThe suitability of high‐resolution two‐dimensional gel electrophoresis
for barley cultivar discrimination and for classification with respect to their
malting properties was studied. Seed proteins of 14 barley cultivars with
different malting qualities were extracted with urea/dithiothreitol/Nonidet P‐40
buffer and subjected to two‐dimensional gel electrophoresis with immobilized pH
gradie...
The proteome of lentil (Lens culinaris Medik.) seeds: Discriminating between landraces
Electrophoresis - Tập 31 Số 3 - Trang 497-506 - 2010
AbstractLentil (Lens culinaris Medik.) is one of the most ancient crops of the
Mediterranean region used for human nutrition; an extensive differentiation of
L. culinaris over millennia has resulted in a number of different landraces. As
a consequence of environmental and socio‐economic issues, the disappearance of
many of them occurred in more recent times. To investigate the potential of
proteom...
Quantitation of microgram amounts of protein in two‐dimensional polyacrylamide gel electrophoresis sample buffer
Electrophoresis - Tập 6 Số 11 - Trang 559-563 - 1985
AbstractThe concentration of protein actually solubilized in sample buffer in
preparation for analysis by two‐dimensional polyacrylamide gel electrophoresis
cannot be directly determined by the Lowry, Biuret, or Bradford protein methods
due to interference by the combinational effect of presence of urea, detergents,
carrier ampholytes, and thiol compounds in sample solubilization buffers.
Determin...
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
Electrophoresis - Tập 8 Số 2 - Trang 93-99 - 1987
AbstractA new modification of silver staining is presented which utilizes two
chemical properties of thiosulfate: image enhancement by pretreatment of fixed
gels, and formation of soluble silver complexes which prevents unspecific
background staining during image development. This procedure provides high
sensitivity for proteins, RNA and DNA in the nanogram range on a colorless,
transparent backgr...
Solubilization of cellular membrane proteins fromStreptococcus mutans for two-dimensional gel electrophoresis
Electrophoresis - Tập 26 Số 6 - Trang 1200-1205 - 2005
Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis
Electrophoresis - Tập 19 Số 5 - Trang 837-844 - 1998
AbstractWe describe the extraction and enrichment of membrane proteins for
separation by two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE)
after differential solubilization of an Escherichia coli cell lysate. In a
simple three‐step sequential solubilization protocol applicable for whole cell
lysates, membrane proteins are partitioned from other cellular proteins by their
insolubility i...
New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis
Electrophoresis - Tập 19 Số 11 - Trang 1901-1909 - 1998
AbstractSevere quantitative loss of protein is often observed in high‐resolution
two‐dimensional electrophoresis of membrane proteins, while the resolution is
usually not affected. To improve the solubility of proteins in this technique,
we tested denaturing cocktails containing various detergents and chaotropes.
Best results were obtained with a denaturing solution containing urea, thiourea,
and ...
Solubilisation of the armadillo‐repeat protein β‐catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE
Electrophoresis - Tập 33 Số 12 - Trang 1804-1813 - 2012
β‐catenin is a member of the armadillo repeat family of proteins and has
important functions in cell–cell adhesion and Wnt signalling. Different protein
species of β‐catenin have been shown to exist in the cell and the relative
proportions of these species are altered upon stimulation of cells with Wnt‐3a
(Gottardi and Gumbiner, 2004). In order to determine whether posttranslational
modifications ...
Chiral ligand exchange micellar electrokinetic chromatography using borate anion as a central ion
Electrophoresis - Tập 26 Số 20 - Trang 3884-3889 - 2005
Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis
Electrophoresis - Tập 26 Số 9 - Trang 1732-1736 - 2005
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