Electrophoresis

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Two–dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: Discrimination of cultivars with different malting grades
Electrophoresis - Tập 13 Số 1 - Trang 192-203 - 1992
Angelika Görg, Wilhelm Postel, Max Baumer, Walter Weiss
AbstractThe suitability of high‐resolution two‐dimensional gel electrophoresis for barley cultivar discrimination and for classification with respect to their malting properties was studied. Seed proteins of 14 barley cultivars with different malting qualities were extracted with urea/dithiothreitol/Nonidet P‐40 buffer and subjected to two‐dimensional gel electroph...... hiện toàn bộ
The proteome of lentil (Lens culinaris Medik.) seeds: Discriminating between landraces
Electrophoresis - Tập 31 Số 3 - Trang 497-506 - 2010
Gabriella Stefania Scippa, Mariapina Rocco, M. Ialicicco, Dalila Trupiano, Vincenzo Viscosi, Michela Di Michele, Simona Arena, Donato Chiatante, Andrea Scaloni
AbstractLentil (Lens culinaris Medik.) is one of the most ancient crops of the Mediterranean region used for human nutrition; an extensive differentiation of L. culinaris over millennia has resulted in a number of different landraces. As a consequence of environmental and socio‐economic issues, the disappearance...... hiện toàn bộ
Quantitation of microgram amounts of protein in two‐dimensional polyacrylamide gel electrophoresis sample buffer
Electrophoresis - Tập 6 Số 11 - Trang 559-563 - 1985
Louis S. Ramagli, Lewis V. Rodriguez
AbstractThe concentration of protein actually solubilized in sample buffer in preparation for analysis by two‐dimensional polyacrylamide gel electrophoresis cannot be directly determined by the Lowry, Biuret, or Bradford protein methods due to interference by the combinational effect of presence of urea, detergents, carrier ampholytes, and thiol compounds in sample...... hiện toàn bộ
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
Electrophoresis - Tập 8 Số 2 - Trang 93-99 - 1987
Helmut Blum, Hildburg Beier, H. Groß
AbstractA new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development. This procedure provides high sensitivity for proteins, RNA and DNA in the nanogram range o...... hiện toàn bộ
Solubilization of cellular membrane proteins fromStreptococcus mutans for two-dimensional gel electrophoresis
Electrophoresis - Tập 26 Số 6 - Trang 1200-1205 - 2005
Kheir Zuobi-Hasona, Paula J. Crowley, Adnan Hasona, Arnold S. Bleiweis, L. Jeannine Brady
Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis
Electrophoresis - Tập 19 Số 5 - Trang 837-844 - 1998
Mark P. Molloy, Ben Herbert, Bradley J. Walsh, Margaret I. Tyler, Mathew Traini, Jean‐Charles Sanchez, Denis F. Hochstrasser, Keith L. Williams, Andrew A. Gooley
AbstractWe describe the extraction and enrichment of membrane proteins for separation by two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) after differential solubilization of an Escherichia coli cell lysate. In a simple three‐step sequential solubilization protocol applicable for whole cell lysates, membrane proteins are part...... hiện toàn bộ
New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis
Electrophoresis - Tập 19 Số 11 - Trang 1901-1909 - 1998
Mireille Chevallet, Alexandra Poinas, David Rouquié, Alexandra Fuchs, Sylvie Kieffer, Michel Galinier, Joël Lunardi, Jérôme Garin, Thierry Rabilloud
AbstractSevere quantitative loss of protein is often observed in high‐resolution two‐dimensional electrophoresis of membrane proteins, while the resolution is usually not affected. To improve the solubility of proteins in this technique, we tested denaturing cocktails containing various detergents and chaotropes. Best results were obtained with a denaturing solutio...... hiện toàn bộ
Solubilisation of the armadillo‐repeat protein β‐catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE
Electrophoresis - Tập 33 Số 12 - Trang 1804-1813 - 2012
Meredith J. Layton, Nicole Church, Maree C. Faux, Hong Ji, Robert J. A. Goode, Eugene A. Kapp, Antony W. Burgess, Richard J. Simpson
β‐catenin is a member of the armadillo repeat family of proteins and has important functions in cell–cell adhesion and Wnt signalling. Different protein species of β‐catenin have been shown to exist in the cell and the relative proportions of these species are altered upon stimulation of cells with hiện toàn bộ
Chiral ligand exchange micellar electrokinetic chromatography using borate anion as a central ion
Electrophoresis - Tập 26 Số 20 - Trang 3884-3889 - 2005
Shuji Kodama, Atsushi Yamamoto, Reiko Iio, Sen‐ichi Aizawa, Kazuya Nakagomi, Kazuichi Hayakawa
Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis
Electrophoresis - Tập 26 Số 9 - Trang 1732-1736 - 2005
Jianzhen Kang, Xue‐Bo Yin, Xiurong Yang, Erkang Wang
Tổng số: 159   
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