A compactly integrated laser‐induced fluorescence detector for microchip electrophoresis

Electrophoresis - Tập 25 Số 12 - Trang 1907-1915 - 2004
Hai‐Fang Li1, Jin‐Ming Lin1, Rongbing Su1, Katsumi Uchiyama2, Toshiyuki Hobo2
1Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
2Department of Applied Chemistry, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan

Tóm tắt

AbstractA simple and easy‐to‐use integrated laser‐induced fluorescence detector for microchip electrophoresis was constructed and evaluated. The fluid channels and optical fiber channels in the glass microchip were fabricated using standard photolithographic techniques and wet chemical etching. A 473 nm diode‐pumped laser was used as the excitation source, and the collimation and collection optics and mirrors were discarded by using a multimode optical fiber to couple the excitation light straight into the microchannel and placing the microchip directly on the top of the photomultiplier tube. A combination of filter systems was incorporated into a poly(dimethylsiloxane) layer, which was reversibly sealed to the bottom of the microchip to eliminate the scattering excitation light reaching to the photomultiplier tube. Fluorescein/calcein samples were taken as model analytes to evaluate the performance with respect to design factors. The detection limits were 0.05 μM for fluorescein and 0.18 μM for calcein, respectively. The suitability of this simple detector for fluorescence detection was demonstrated by baseline separation of fluorescein isothiocyanate (FITC)‐labeled arginine, phenylalanine, and glycine and FITC within 30 s at separation length of 3.8 cm and electrical field strength of 600 V/cm.

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Electrophoresis

Tập 25 Số 12

1907-1915

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