Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels
Tóm tắt
A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05–0.1 ng protein per band. Total staining time with the new procedure is 75 min.
Từ khóa
Tài liệu tham khảo
Heukeshoven J., 1986, Elektrophorese Forum '86, 22
Brewer J., 1986, Electrophoresis '86, 226
PhastSystem Owner's Manual Pharmacia Uppsala Sweden.
PhastSystem Development Technique File No. 210 Pharmacia Uppsala Sweden.
PhastSystem Separation Technique File No. 100 Pharmacia Uppsala Sweden.
Heukeshoven J., 1987, Elektrophorese Forum '87, 208
Dernick R., 1986, Elektrophorese Forum '86, 107