Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels

Electrophoresis - Tập 9 Số 1 - Trang 28-32 - 1988
Jochen Heukeshoven1, Rudolf Dernick1
1Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg

Tóm tắt

AbstractA new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05–0.1 ng protein per band. Total staining time with the new procedure is 75 min.

Từ khóa


Tài liệu tham khảo

10.1002/elps.1150060302

Heukeshoven J., 1986, Elektrophorese Forum '86, 22

10.1002/elps.1150070708

10.1016/0003-2697(81)90783-1

10.1002/elps.1150080308

Brewer J., 1986, Electrophoresis '86, 226

PhastSystem Owner's Manual Pharmacia Uppsala Sweden.

PhastSystem Development Technique File No. 210 Pharmacia Uppsala Sweden.

PhastSystem Separation Technique File No. 100 Pharmacia Uppsala Sweden.

Heukeshoven J., 1987, Elektrophorese Forum '87, 208

10.1002/elps.1150030104

10.1002/elps.1150030404

10.1016/0003-2697(83)90057-X

10.1016/0003-2697(83)90714-5

Dernick R., 1986, Elektrophorese Forum '86, 107

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Thông tin xuất bản

Electrophoresis

Tập 9 Số 1

28-32

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