Cytogenetic and Genome Research

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Assignment of the gene encoding human Krüppel-related zinc finger protein 4 (GLI4) to 8q24.3 by fluorescent in situ hybridization
Cytogenetic and Genome Research - Tập 72 Số 4 - Trang 297-298 - 1996
Koen Kas, Iwona Włodarska, E. Meyen, Herman Van den Berghe, Wim J.M. Van de Ven
The gene for human Krüppel-related protein 4, (HKR4, gene symbol GLI4), a zinc finger protein of unknown function, has been localized by fluorescence in situ hybridization to chromosome 8q24.3, distal to c-MYC.
Consequences of polyspermy in man
Cytogenetic and Genome Research - Tập 42 Số 1-2 - Trang 1-7 - 1986
R.R. Angell, Allan Templeton, Ioannis E. Messinis
Polyspermy occurs with a frequency of about 12% under some hormonal regimens currently being used for in vitro fertilization and embryo transfer. Oocytes with three pronuclei usually show normal cleavage and are morphologically indistinguishable from embryos produced by normal fertilization. Chromosome studies and DNA measurements on dispermic embryos show that about one-half are triploid,...... hiện toàn bộ
Chromosomal localization of the human oncogene <i>ERBA2</i>
Cytogenetic and Genome Research - Tập 43 Số 3-4 - Trang 150-153 - 1986
John R. Gosden, Peter G. Middleton, Derek Rout, Carmine De Angelis
The human <i>ERBA2 </i>gene has been mapped to chromosome 17q21.3 by in situ hybridization. A second hybridization site at 17q25 was greatly reduced by high stringency washes, indicating the presence of an additional member of the <i>ERBA2 </i>family at this location. Southern blot hybridization using identical conditions did not reveal additional bands, and hybridi...... hiện toàn bộ
Hybrid genome evolution by transposition
Cytogenetic and Genome Research - Tập 110 Số 1-4 - Trang 49-55 - 2005
Antonio Fontdevila
Species hybridization is reviewed focusing on its role as a source of evolutionary novelties. Contrary to the view that hybrids are lineages devoid of evolutionary value, a number of case studies are given that show how hybrids are responsible for reticulate evolution that may lead to the origin of new species. Hybrid evolution is mediated by extensive genome repatterning followed by rapid...... hiện toàn bộ
TET Family of Dioxygenases: Crucial Roles and Underlying Mechanisms
Cytogenetic and Genome Research - Tập 146 Số 3 - Trang 171-180 - 2015
Duo Li, Bin Guo, Jing Wang, Lina Tan, Qianjin Lu
DNA methylation plays an important role in the epigenetic regulation of mammalian gene expression. TET (ten-eleven translocation) proteins, newly discovered demethylases, have sparked great interest since their discovery. TET proteins catalyze 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine in 3 consecutive Fe(II)- and 2-oxoglutarate (2-OG)-dependent ox...... hiện toàn bộ
Limitations of in situ hybridization with total genomic DNA in routine screening for alien introgressions in wheat
Cytogenetic and Genome Research - Tập 109 Số 1-3 - Trang 373-377 - 2005
Adam J. Lukaszewski, B. Łapiński, Krystyna Rybka
In situ hybridization with total genomic DNA (GISH) has become a powerful tool in characterization of alien introgressions in wheat. With recent simplification it can now be used in large scale screening for new chromosome constructs. Its level of resolution in routine applications was tested on sets of recombined wheat-rye chromosomes with genetically determined positions of the transloca...... hiện toàn bộ
Confirmed assignment of a novel human tyrosine kinase gene (JAK1A) to 1p32.3→p31.3 by nonisotopic in situ hybridization
Cytogenetic and Genome Research - Tập 69 Số 3-4 - Trang 232-234 - 1995
William S. Modi, William L. Farrar, O. M. Zack Howard
JAK1A is a recently isolated class 3 (nonreceptor) tyrosine kinase that has catalytic domain sequence homology with other kinases and is known to be ubiquitously expressed in all human tissues thus far examined. The gene for this enzyme had previously been localized to chromosome 1 using somatic cell hybrids and linkage analyses. In the present study, fluorescence in situ hybridization was...... hiện toàn bộ
Organization and expression of rat <i>Tspy</i>
Cytogenetic and Genome Research - Tập 83 Số 3-4 - Trang 270-274 - 1998
F. Dechend, Stephanie Schubert, Indrajit Nanda, Tanja Vogel, Michael Schmid, Jörg Schmidtke
We have isolated both a full-length rat <i>Tspy </i>cDNA from testicular mRNA by <b>r</b>apid <b>a</b>mplification of <b>c</b>DNA <b>e</b>nds (RACE) and RT-PCR and a full-length rat <i>Tspy </i>gene from genomic DNA by PCR. In contrast to the mouse, where <i>Tspy </i>is present in a single copy and is appar...... hiện toàn bộ
An Air-Drying Method for Meiotic Preparations from Mammalian Testes
Cytogenetic and Genome Research - Tập 3 Số 5 - Trang 289-294 - 1964
E. P. Evans, G. Breckon, C. E. Ford
A suspension is made in isotonic (2.2%) sodium citrate solution from the contents of the tubules from a whole testis or a testicular biopsy specimen. The germinal cells are sedimented by centrifuging, leaving most of the sperm in the supernatant fluid, which is discarded. The cells are resuspended in hypo-tonic (1%) sodium citrate solution and left to stand at room temperature for 12 minut...... hiện toàn bộ
Human AQP2 and MIP genes, two members of the MIP family, map within chromosome band 12q13 on the basis of two-color FISH
Cytogenetic and Genome Research - Tập 68 Số 1-2 - Trang 45-48 - 1995
Fumiko Saito, Shiro Sasaki, A.B. Chepelinsky, Kiyohide Fushimi, Fumiaki Marumo, Tatsuro Ikeuchi
The human AQP2 (collecting duct water channel, aquaporin 2) gene encodes a 271 amino acid protein and is a member of the MIP (major intrinsic protein of lens fiber) gene family. Using two-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for AQP2 and MIP as probes, we found that both genes mapped closely within the human chromosom...... hiện toàn bộ
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