F. Dechend1, Stephanie Schubert, Indrajit Nanda, Tanja Vogel, Michael Schmid, Jörg Schmidtke
1Institut für Humangenetik, Medizinische Hochschule, Hannover, Germany
Tóm tắt
We have isolated both a full-length rat <i>Tspy </i>cDNA from testicular mRNA by <b>r</b>apid <b>a</b>mplification of <b>c</b>DNA <b>e</b>nds (RACE) and RT-PCR and a full-length rat <i>Tspy </i>gene from genomic DNA by PCR. In contrast to the mouse, where <i>Tspy </i>is present in a single copy and is apparently functionless, and to man and cattle, where TSPY is organized in a moderately repetitive cluster, the rat <i>Tspy </i>locus apparently consists of one complete functional and one truncated, probably nonfunctional, copy, coherently localized on Yp, as revealed by FISH analysis.
