ChemBioChem
SCOPUS (2000-2025)SCIE-ISI
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Cơ quản chủ quản: WILEY-V C H VERLAG GMBH , Wiley-VCH Verlag
Lĩnh vực:
BiochemistryMolecular MedicineOrganic ChemistryMolecular Biology
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Các bài báo tiêu biểu
Real‐Time Polymerase Chain Reaction
Tập 4 Số 11 - Trang 1120-1128 - 2003
AbstractReal‐time PCR is the state‐of‐the‐art technique to quantify nucleic
acids for mutation detection, genotyping and chimerism analysis. Since its
development in the 1990s, many different assay formats have been developed and
the number of real‐time PCR machines of different design is continuously
increasing. This review provides a survey of the instruments and assay formats
available and disc...
Insight Into the Kinetic of Amyloid β (1–42) Peptide Self‐Aggregation: Elucidation of Inhibitors’ Mechanism of Action
Tập 8 Số 17 - Trang 2152-2161 - 2007
AbstractThe initial transition of amyloid β (1–42) (Aβ42) soluble monomers/small
oligomers from unordered/α‐helix to a β‐sheet‐rich conformation represents a
suitable target to design new potent inhibitors and to obtain effective
therapeutics for Alzheimer's disease. Under optimized conditions, this reliable
and reproducible CD kinetic study showed a three‐step sigmoid profile that was
characteriz...
NMR Spectroscopy of Paramagnetic Metalloproteins
Tập 6 Số 9 - Trang 1536-1549 - 2005
AbstractThis article deals with the solution structure determination of
paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed
not to be suitable for NMR investigations for structure determination until a
decade ago, but eventually novel experiments and software protocols were
developed, with the aim of making the approach suitable for the goal and as
user‐friendly and safe...
Directed Evolution of Enzymes for Industrial Biocatalysis
Tập 17 Số 3 - Trang 197-203 - 2016
AbstractEnzymes have the potential to catalyse a wide variety of chemical
reactions. They are increasingly being sought as environmentally friendly and
cost‐effective alternatives to conventional catalysts used in industries ranging
from bioremediation to applications in medicine and pharmaceutics. Despite the
benefits, they are not without their limitations. Many naturally occurring
enzymes are n...
Evaluation of Lipopolysaccharide Aggregation by Light Scattering Spectroscopy
Tập 4 Số 1 - Trang 96-100 - 2003
AbstractLipopolysaccharides (LPS) are cell wall components of Gram‐negative
bacteria. These molecules behave as bacterial endotoxins and their release into
the bloodstream is a determinant of the development of a wide range of
pathologies. These amphipathic molecules can self‐aggregate into supramolecular
structures with different shapes and sizes. The formation of these structures
occurs when the...
Selection of D ‐Amino‐Acid Peptides That Bind to Alzheimer's Disease Amyloid Peptide Aβ1–42 by Mirror Image Phage Display
Tập 4 Số 8 - Trang 748-753 - 2003
AbstractA mirror image phage display approach was used to identify novel and
highly specific ligands for Alzheimer's disease amyloid peptide Aβ(1–42). A
randomized 12‐mer peptide library presented on M13 phages was screened for
peptides with binding affinity for the mirror image of Aβ(1–42). After four
rounds of selection and amplification the peptides were enriched with a
dominating consensus seq...
Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications
Tập 19 Số 1 - Trang 7-21 - 2018
AbstractPichia pastoris is a well‐known platform strain for heterologous protein
expression. Over the past five years, different strategies to improve the
efficiency of recombinant protein expression by this yeast strain have been
developed; these include a patent‐free protein expression kit, construction of
the P. pastoris CBS7435Ku70 platform strain with its high efficiency in
site‐specific reco...
Exosomes and Microvesicles: Identification and Targeting By Particle Size and Lipid Chemical Probes
Tập 15 Số 7 - Trang 923-928 - 2014
AbstractExosomes and microvesicles are two classes of submicroscopic vesicle
released by cells into the extracellular space. Collectively referred to as
extracellular vesicles, these membrane containers facilitate important cell–cell
communication by carrying a diverse array of signaling molecules, including
nucleic acids, proteins, and lipids. Recently, the role of extracellular vesicle
signaling...
Rational Design of a Minimal and Highly Enriched CYP102A1 Mutant Library with Improved Regio‐, Stereo‐ and Chemoselectivity
Tập 10 Số 5 - Trang 853-861 - 2009
AbstractMonooxygenase mutants: A minimal and highly enriched CYP102A1 mutant
library was constructed by combining five hydrophobic amino acids in two
positions. The library was screened with four different terpene substrates.
Eleven variants demonstrated either a strong shift or improved regio‐ or
stereoselectivity during oxidation of at least one substrate as compared to
CYP102A1 wild type.magnif...