ChemBioChem
SCOPUS (2000-2023)SCIE-ISI
1439-4227
1439-7633
Đức
Cơ quản chủ quản: WILEY-V C H VERLAG GMBH , Wiley-VCH Verlag
Lĩnh vực:
BiochemistryMolecular MedicineOrganic ChemistryMolecular Biology
Các bài báo tiêu biểu
Real‐Time Polymerase Chain ReactionAbstract Real‐time PCR is the state‐of‐the‐art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real‐time PCR machines of different design is continuously increasing. This review provides a survey of the in...
Tập 4 Số 11 - Trang 1120-1128 - 2003
Insight Into the Kinetic of Amyloid β (1–42) Peptide Self‐Aggregation: Elucidation of Inhibitors’ Mechanism of ActionAbstract The initial transition of amyloid β (1–42) (Aβ42) soluble monomers/small oligomers from unordered/α‐helix to a β‐sheet‐rich conformation represents a suitable target to design new potent inhibitors and to obtain effective therapeutics for Alzheimer's disease. Under optimized conditions, this reliable and reproducible CD kinetic study showed a three‐step sig... ...
Tập 8 Số 17 - Trang 2152-2161 - 2007
NMR Spectroscopy of Paramagnetic MetalloproteinsAbstract This article deals with the solution structure determination of paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed not to be suitable for NMR investigations for structure determination until a decade ago, but eventually novel experiments and software protocols were developed, with the aim of making the approach suita...
Tập 6 Số 9 - Trang 1536-1549 - 2005
Directed Evolution of Enzymes for Industrial BiocatalysisAbstract Enzymes have the potential to catalyse a wide variety of chemical reactions. They are increasingly being sought as environmentally friendly and cost‐effective alternatives to conventional catalysts used in industries ranging from bioremediation to applications in medicine and pharmaceutics. Despite the benefits, they are not without their limitations. Many ... ...
Tập 17 Số 3 - Trang 197-203 - 2016
Evaluation of Lipopolysaccharide Aggregation by Light Scattering SpectroscopyAbstract Lipopolysaccharides (LPS) are cell wall components of Gram‐negative bacteria. These molecules behave as bacterial endotoxins and their release into the bloodstream is a determinant of the development of a wide range of pathologies. These amphipathic molecules can self‐aggregate into supramolecular structures with different shapes and sizes. The...
Tập 4 Số 1 - Trang 96-100 - 2003
Selection of D ‐Amino‐Acid Peptides That Bind to Alzheimer's Disease Amyloid Peptide Aβ1–42 by Mirror Image Phage DisplayAbstract A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide Aβ(1–42). A randomized 12‐mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of Aβ(1–42). After four rounds of selection and amplification the peptides...
Tập 4 Số 8 - Trang 748-753 - 2003
Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and ApplicationsAbstract Pichia pastoris is a well‐known platform strain for heterologous protein expression. Over the past five years, different strategies to improve the efficiency of recombinant protein expression by this yeast strain have been developed; these include a patent‐free protein expression kit, construction of the P. pastoris...
Tập 19 Số 1 - Trang 7-21 - 2018
Exosomes and Microvesicles: Identification and Targeting By Particle Size and Lipid Chemical ProbesAbstract Exosomes and microvesicles are two classes of submicroscopic vesicle released by cells into the extracellular space. Collectively referred to as extracellular vesicles, these membrane containers facilitate important cell–cell communication by carrying a diverse array of signaling molecules, including nucleic acids, proteins, and lipids. Recently, the role o... ...
Tập 15 Số 7 - Trang 923-928 - 2014
Rational Design of a Minimal and Highly Enriched CYP102A1 Mutant Library with Improved Regio‐, Stereo‐ and ChemoselectivityAbstract Monooxygenase mutants : A minimal and highly enriched CYP102A1 mutant library was constructed by combining five hydrophobic amino acids in two positions. The library was screened with four different terpene substrates. Eleven variants demonstrated either a strong shift or improved regio‐ or stereoselectivity during oxidation of at leas...
Tập 10 Số 5 - Trang 853-861 - 2009